Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	32 [0]	[0] 32	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	28 (0.820)	23/266	0.6	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

ATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_pae_tpiA/1978359‑1978650                                                                                                                                                |                                                                                                                                                     aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                                 <  1:349372‑M1/149‑6 (MQ=255) aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACGCAATTACTTAACATAAATGtataa                                                                                                                                                 <  2:507742‑M1/149‑6 (MQ=255)  tGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGGACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACACAATTACTTAACATAAATGtataag                                                                                                                                                >  1:625391‑M1/1‑143 (MQ=255)            cacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGCTTTTCCATACTGCTCGATGCAGATCCCACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                                      <  2:611466‑M1/149‑17 (MQ=255)                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                               <  2:572099‑M1/149‑24 (MQ=255)                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                               <  2:55717‑M1/149‑24 (MQ=255)                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                               <  1:616095‑M1/149‑24 (MQ=255)                    tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                               >  1:220283‑M1/1‑125 (MQ=255)                    tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                               <  2:220283‑M1/148‑24 (MQ=255)                               cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAATTGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                   <  1:58717‑M1/149‑36 (MQ=255)                                    tCGACGCAACTGTACTCGTCACTACACGCCAATACACCGGAGCGTGGCTGCGAATTTCAATAATGCGTGATGCAGATCACACAAAACACTCCATTACTTAACATAAATGtataagtcatactcttgttttgggtgtatttcaatctgtt                                                                                                              <  2:294098‑M1/149‑41 (MQ=255)                                                         cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                         >  1:425842‑M1/1‑88 (MQ=255)                                                             acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                                     >  1:599227‑M1/1‑84 (MQ=255)                                                                 acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                                  >  1:551625‑M1/1‑80 (MQ=255)                                                                 acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                                  <  2:551625‑M1/132‑53 (MQ=255)                                                                      cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatg                                                                            >  2:607955‑M1/1‑75 (MQ=255)                                                                         cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctaagctagaaagcaaaaatgaaa                                                                         <  2:144972‑M1/149‑78 (MQ=255)                                                                               ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaa                                                                   >  1:244940‑M1/1‑66 (MQ=255)                                                                               ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaa                                                                   <  1:57901‑M1/149‑84 (MQ=255)                                                                                  gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACAATCAATTTTTTAACAGAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                                <  2:480169‑M1/149‑87 (MQ=255)                                                                                   cTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                               >  1:364896‑M1/1‑62 (MQ=255)                                                                                              aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                                    >  1:311899‑M1/1‑51 (MQ=255)                                                                                                           gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatatgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatagaaaagggatgttgcgcacagtc                                       >  2:13309‑M1/1‑38 (MQ=255)                                                                                                                tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaat                                  >  1:373590‑M1/1‑33 (MQ=255)                                                                                                                    acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtctatttcaatctgttaaaaagtttttcgctacgctagcaagcaagaatgaaaaagaaataatcgaaatgggatgttgtggacagtcaaaataact                              >  2:408163‑M1/1‑29 (MQ=255)                                                                                                                    acaAAACACACAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaaaggaataatcgaaatgggatgttgcgcacagtcaaaaaaact                              >  2:415588‑M1/1‑29 (MQ=255)                                                                                                                       aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaa                           >  1:635540‑M1/1‑26 (MQ=255)                                                                                                                               aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgcaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaata                   >  2:142188‑M1/1‑18 (MQ=255)                                                                                                                                       aatataaATGtataagtcatacttttaatttgggggtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagccaaaataactcaccgtaaataatcttctg           <  2:451656‑M1/146‑140 (MQ=255)                                                                                                                                         cATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgct         <  2:599227‑M1/149‑142 (MQ=255)                                                                                                                                          aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta        >  1:409879‑M1/1‑7 (MQ=255)                                                                                                                                                gtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaata  >  1:538907‑M1/1‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     ATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_pae_tpiA/1978359‑1978650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑