Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	32 [0]	[0] 32	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	28 (0.820)	23/266	0.6	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATA  >  NC_000913_3_pae_tpiA/1979135‑1979426                                                                                                                                                 |                                                                                                                                                    atgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATaa                                                                                                                                                 <  1:349372‑M2/5‑1 (MQ=255) atgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacacgcaattacttaacataaatgTATaa                                                                                                                                                 <  2:507742‑M2/5‑1 (MQ=255)  tgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacggacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacacacaattacttaacataaatgTATAAg                                                                                                                                                >  1:625391‑M2/144‑149 (MQ=255)            cacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgcttttccatactgctcgatgcagatcccacaaaacactcaattacttaacataaatgTATAAGTCATACtttt                                                                                                                                      <  2:611466‑M2/16‑1 (MQ=255)                   ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                               <  2:572099‑M2/23‑1 (MQ=255)                   ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                               <  2:55717‑M2/23‑1 (MQ=255)                   ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                               <  1:616095‑M2/23‑1 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                               >  1:220283‑M2/126‑148 (MQ=255)                    taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                               <  2:220283‑M2/23‑1 (MQ=255)                               ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataattgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                                   <  1:58717‑M2/35‑1 (MQ=255)                                    tcgacgcaactgtactcgtcactacacgccaatacaccggagcgtggctgcgaatttcaataatgcgtgatgcagatcacacaaaacactccattacttaacataaatgTATAAGTCATACTCTTGTTTTGGGTGTATTTCAATCTGtt                                                                                                              <  2:294098‑M2/40‑1 (MQ=255)                                                         ctacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgcta                                                                                         >  1:425842‑M2/89‑149 (MQ=255)                                                             acgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                     >  1:599227‑M2/85‑149 (MQ=255)                                                                 acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                  >  1:551625‑M2/81‑132 (MQ=255)                                                                 acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                                                                  <  2:551625‑M2/52‑1 (MQ=255)                                                                      caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATg                                                                            >  2:607955‑M2/76‑149 (MQ=255)                                                                         cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTAAGCTAGAAAGCAAAAATGaaa                                                                         <  2:144972‑M2/77‑1 (MQ=255)                                                                               ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                                   >  1:244940‑M2/67‑149 (MQ=255)                                                                               ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                                   <  1:57901‑M2/83‑1 (MQ=255)                                                                                  gctgcgattttcaataatgcgtgatgcagatcacacaaaacaatcaattttttaacagaaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAG‑TTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                               <  2:480169‑M2/86‑1 (MQ=255)                                                                                   ctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat                                                               >  1:364896‑M2/63‑149 (MQ=255)                                                                                              aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt                                                    >  1:311899‑M2/52‑149 (MQ=255)                                                                                                           gcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATATGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATAGAAAAGGGATGTTGCGCACAGTc                                       >  2:13309‑M2/39‑149 (MQ=255)                                                                                                                tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAAt                                  >  1:373590‑M2/34‑149 (MQ=255)                                                                                                                    acaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTCTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAGAATGAAAAAGAAATAATCGAAATGGGATGTTGTGGACAGTCAAAATAACt                              >  2:408163‑M2/30‑149 (MQ=255)                                                                                                                    acaaaacacacaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAAAAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAAAAACt                              >  2:415588‑M2/30‑149 (MQ=255)                                                                                                                       aaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTcaa                           >  1:635540‑M2/27‑148 (MQ=255)                                                                                                                               aattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGCAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaata                   >  2:142188‑M2/19‑149 (MQ=255)                                                                                                                                       aatataaatgTATAAGTCATACTTTTAATTTGGGGGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGCCAAAATAACTCACCGTAAATAATCTTCTg           <  2:451656‑M2/139‑1 (MQ=255)                                                                                                                                         cataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCt         <  2:599227‑M2/141‑1 (MQ=255)                                                                                                                                          ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta        >  1:409879‑M2/8‑149 (MQ=255)                                                                                                                                                gTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaata  >  1:538907‑M2/2‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                    ATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATA  >  NC_000913_3_pae_tpiA/1979135‑1979426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑