New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 8228943 (1.090)5 (0.130) 5/266 4.3 10.9% coding (1241/1572 nt) leuA 2‑isopropylmalate synthase
?NC_000913_3_pae_tpiA = 82309 41 (1.100)coding (1221/1572 nt) leuA 2‑isopropylmalate synthase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/82136‑82289
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgccacCGCCGCCGTCAAACTGGCCTGTGGCGAAGAAGTCAAAGCAGAAGCCGCCAACGGTAACGGTCCGGTCGATGCCGTCTATCAGGCAATTAACCGCA  <  NC_000913_3_pae_tpiA/82309‑82215
                                                                                                                                                                                                                                                         
cCCACCTGCCCCAGCGCATCGTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTC                                                                                                      <  2:666269/148‑1
TCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGACAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGCCCCTTACGATTGGCGTCTTCTGCTTTGACGTCTTC                                                                                                      >  1:14191/1‑149
          CCAGCGCATCGTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTGTTCGCCCCCGgcc                                                                                            >  1:680946/1‑146
                                                           TTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACCGCCGCCGTCAAACTGGCCTGTGGC                                                                        <  1:64440/120‑1
                                                           TTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACCGCCGCCGTCAAACTGGCCTGTGGC                                                                        >  2:64440/1‑120
                                                                 GTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACCGCCGCCGTCAAACTGGCCTGTGGCGAAGAAGT                                                                >  1:665268/1‑122
                                                                 GTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACCGCCGCCGTCAAACTGGCCTGTGGCGAAGAAGT                                                                <  2:665268/122‑1
                                                                                                                                                   TCGCCACCGCCGCCGTCAAACTGGCCTGTGGCTAAGAAGTCAAAGCAGAAGCCGCCAATGGTAACGGTCCGGTCGATGCCGTCTATCAGGCAATTAACCGCA  <  1:534073/102‑1
                                                                                                                                                   TCGCCACCGCCGCCGTCAAACTGGCCTGTGGCGAAGAAGTCAAAGCAGAAGCCGCCAACGGTAACGGTCCGGTCGATGCCGTCTATCAGGCAATTAACCGCA  >  2:534073/1‑102
                                                                                                                                                                                                                                                         
TCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/82136‑82289
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgccacCGCCGCCGTCAAACTGGCCTGTGGCGAAGAAGTCAAAGCAGAAGCCGCCAACGGTAACGGTCCGGTCGATGCCGTCTATCAGGCAATTAACCGCA  <  NC_000913_3_pae_tpiA/82309‑82215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.