New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 82289	43 (1.090)	5 (0.130)	5/266	4.3	10.9%	coding (1241/1572 nt)	leuA	2‑isopropylmalate synthase
	?	NC_000913_3_pae_tpiA	= 82309	41 (1.100)					coding (1221/1572 nt)	leuA	2‑isopropylmalate synthase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTT  >  NC_000913_3_pae_tpiA/82143‑82430                                                                                                                                                   |                                                                                                                                              ggctcAGCGCATCTTGACCCTTGCCTTTTGCGGGCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAg                                                                                                                                             <  2:19323/145‑1 (MQ=255) gACCCAGCGCATCTTTCCCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAg                                                                                                                                             <  1:537235/149‑1 (MQ=255)    ccAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGcc                                                                                                                                          >  1:108506/1‑149 (MQ=255)    ccAGCGCATCGTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTGTTCGCCCCCGGcc                                                                                                                                          >  1:680946/1‑149 (MQ=255)                tACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATAATCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGAcagag                                                                                                                              >  1:274383/1‑145 (MQ=255)                    gTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGAcggcggcag                                                                                                                          >  1:540238/1‑147 (MQ=255)                              gCGGTGCGGCTGTATTTCACCAGTTCGACGGTATATTCAGGGATGCGGTTAATTGCCTGATAGACGGCAGCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATc                                                                                                                <  2:297754/149‑1 (MQ=255)                              gCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATc                                                                                                                <  2:108506/149‑1 (MQ=255)                               cGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCg                                                                                                               >  2:248170/1‑149 (MQ=255)                                 gttgggCTGTATTTCACCAGTTAGACGTTATATTCAGTGATGCGGTGAATTGCGTGACAGACGGCATCGACCGGACCGTTACCGTTGGCGGCGTCTGCTTGGACTTCTTCGCCACTTGCCAGTTTGACGGTGGCGGTGGCGATAACGtt                                                                                                             <  2:26438/145‑1 (MQ=255)                                      gCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGc                                                                                                        <  1:151063/149‑1 (MQ=255)                                       ttGTATTTTACCTGTTCGGAGTTCGATGCACTGATGCGTTTAATTGCCTGATAGACGGTAGCGAACGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTATTCGCCACAGGCCAG‑TTGACGGCGGCGGTGGCGATATCGTTAGAGCCa                                                                                                      <  2:433344/148‑1 (MQ=255)                                       cTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTagagac                                                                                                       >  2:639571/1‑147 (MQ=255)                                        tGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCa                                                                                                      >  1:505865/1‑149 (MQ=255)                                          tATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGa                                                                                                    <  2:236939/149‑1 (MQ=255)                                            tttCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGGCACAGTCAAGTTTGAAGGCGGCGGTGGGGATATCGTTAGAGCCAGACt                                                                                                  >  1:93443/1‑149 (MQ=255)                                                  cAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGc                                                                                            >  2:244474/1‑149 (MQ=255)                                                   aGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCCGTTTGACGGCGGCGGTGGCGCTATCGTGTGAGCCAGAATGCCCGCt                                                                                           >  1:498214/1‑149 (MQ=255)                                                      tCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGaa                                                                                        <  1:48971/149‑1 (MQ=255)                                                            ttATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCATGCTGCCCGCTTAAGTAATc                                                                                  >  1:320071/1‑149 (MQ=255)                                                              atatTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCa                                                                                <  2:546339/149‑1 (MQ=255)                                                              atatTCAGTGATGCGGTTAATTGCCAGATAGACGTCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGCCGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCa                                                                                <  1:602292/149‑1 (MQ=255)                                                                         tGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGa                                                                                                                    <  2:534073/102‑1 (MQ=255)                                                                         tGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCATTGGCGGCTTCTGCTTTGACTTCTTAGCCACAGGCCAGTTTGACGGCGGCGGTGGCGa                                                                                                                    >  1:534073/1‑102 (MQ=255)                                                                             gTTAATTGCCTGATCGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCCGTGACTTCTTCGCCCCAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGCACCAGACTGCACGGCGAAGTAAACCAGCCGGACCTGCTCCg                                                                 >  2:338592/1‑149 (MQ=255)                                                                                   tGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTGCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCtctt                                                           <  2:51973/149‑1 (MQ=255)                                                                                    gCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCtcttc                                                          >  1:675617/1‑149 (MQ=255)                                                                                              cGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGTTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTa                                                >  2:150846/1‑149 (MQ=255)                                                                                                  aTCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGa                                            <  1:102953/149‑1 (MQ=255)                                                                                                   tCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGCCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGAt                                           >  1:114344/1‑149 (MQ=255)                                                                                                   tCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGAt                                           <  1:568220/149‑1 (MQ=255)                                                                                                          gaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAAt                                                                       >  1:476589/1‑114 (MQ=255)                                                                                                          gaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAAt                                                                       <  2:476589/114‑1 (MQ=255)                                                                                                             cgttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCGGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAgc                                 >  2:202936/1‑149 (MQ=255)                                                                                                                gaccgttGGCGGCGTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCacggta                              <  2:274383/148‑6 (MQ=255)                                                                                                                  ccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTc                            <  1:343247/149‑1 (MQ=255)                                                                                                                   cgttTGCCGCTTCTGCGTTGCCTTCTTCGCCACAGGCCAGGTTGAACGAGGGGCTGGCAAGATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCGCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTcc                           <  1:132309/149‑1 (MQ=255)                                                                                                                        gcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATc                      >  1:354259/1‑149 (MQ=255)                                                                                                                          ggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGt                    <  2:354259/149‑1 (MQ=255)                                                                                                                           gcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTAAAGATGGCGATCTGCGCCTCCAGTTCGTa                   <  2:505865/149‑1 (MQ=255)                                                                                                                                 gCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTCATCaaa             >  2:319920/1‑149 (MQ=255)                                                                                                                                   tttGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGGTGAAGGCCAGCGCCTCCAGCTCATAATCAAgca           >  2:108534/1‑149 (MQ=255)                                                                                                                                         ttcttcGACACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGAc     <  1:150846/149‑1 (MQ=255)                                                                                                                                            ttcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGCAGGCCAGCGCCTCCAGATCGTACTCAAACAcccaaccat  >  1:71417/1‑142 (MQ=255)                                                                                                                                                   |                                                                                                                                              GACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTT  >  NC_000913_3_pae_tpiA/82143‑82430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.