New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	349375 =	55 (0.990)	5 (0.090)	4/270	7.2	8.0%	coding (694/891 nt)	prpB	2‑methylisocitrate lyase
	?	NC_000913_3_pae_tpiA	349410 =	62 (1.180)					coding (729/891 nt)	prpB	2‑methylisocitrate lyase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAA  >  NC_000913_3_pae_tpiA/349227‑349514                                                                                                                                                     |                                                                                                                                            cGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGCCATGTTCCGCTTGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCaa                                                                                                                                             <  1:272551/149‑1 (MQ=255)       gttgttCCCGGAGGCGATTACCGAACCCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGgcgc                                                                                                                                       <  1:94439/149‑1 (MQ=255)           ttCCCGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGAGGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTa                                                                                                                                   >  1:570472/1‑149 (MQ=255)             ctcGGAGGCGATTACCGAGCTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTAcc                                                                                                                                 <  1:71010/147‑1 (MQ=255)               cGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGGAATCGCGGTGATTACAACCGACGAATTAGGCAGCGCCCATGTCGCAGTGGCGCTGtgaccc                                                                                                                               >  1:477438/1‑144 (MQ=255)               cGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCa                                                                                                                               >  2:401714/1‑149 (MQ=255)                      gATTACCGAACGCGCCATGGACCGCTAGTTTGCCGATGCGGTGCAGGTGCCGAGCCTCTCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAg                                                                                                                        <  2:166717/149‑1 (MQ=255)                        ttACCGAACTCACCATATACCGCCCATTTGCCGATGCGGTGCAGGCGCCTATCCTCGCCAACCTCTCCGTCTTTGGGGCCGCACCGCTGTTTACCACCGATGAATTACGCACCGCCCATTTCGCAATGGCGCTGTACCCACTTTCAGCg                                                                                                                      >  2:889947/1‑149 (MQ=255)                                  cGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCa                                                                                                            >  2:185488/1‑149 (MQ=255)                                  cGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCa                                                                                                            <  1:185488/149‑1 (MQ=255)                                     cATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGa                                                                                                         >  1:747712/1‑149 (MQ=255)                                       tGTACCGCCAGTGTGCCGATGCGGTGCAGGTGCCGAACCTCGCCAACATAACCGAATTTGGTGCCACGCCCCTGTTTACCACCGACGAATTACGCGTCGACCATGACGCAATGGCGCTGTACCCACTTTCAGCGTGCCGCACCAGGaca                                                                                                       >  2:502476/1‑147 (MQ=255)                                              ccAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTAc                                                                                                                                  >  1:158628/1‑115 (MQ=255)                                              ccAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTAc                                                                                                                                  <  2:158628/115‑1 (MQ=255)                                                      ccGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACAt                                                                                        <  2:82947/149‑1 (MQ=255)                                                       cGATGCGGTGCAGGTGCCGATCCTCGCCAACATCCCCGCAGCTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCCCTGAACATg                                                                                       >  2:417041/1‑149 (MQ=255)                                                         aTGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTc                                                                                     <  1:425780/149‑1 (MQ=255)                                                             ggTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACa                                                                                 >  1:133567/1‑149 (MQ=255)                                                                  aGGTGCCGATCCTCGCCAACATCACCGAATTGTGTGTCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTc                                                                            <  1:451539/149‑1 (MQ=255)                                                                   ggTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTcc                                                                           <  2:133567/149‑1 (MQ=255)                                                                    gTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTa                                                                                   <  2:85610/140‑1 (MQ=255)                                                                    gTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTa                                                                                   >  1:85610/1‑140 (MQ=255)                                                                      gCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTgc                                                                        >  1:338341/1‑149 (MQ=255)                                                                        cGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATATCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTgcgc                                                                      >  2:488052/1‑149 (MQ=255)                                                                              tCGCCAACATGACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCAGGTCGCCTGGGCGATGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCGGCTGAACATGTCTACAACGTCCTGCGCCAGGaa                                                                <  2:248671/149‑1 (MQ=255)                                                                              tCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGaa                                                                                                        <  1:552972/109‑1 (MQ=255)                                                                              tCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGaa                                                                                                        >  2:552972/1‑109 (MQ=255)                                                                              tCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGag                                                                >  1:78983/1‑148 (MQ=255)                                                                              tCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCGACAACGTCCTGCGCCAGGaa                                                                >  1:133869/1‑149 (MQ=255)                                                                                          cctaaTTTGGTGCCACGCCGCTGGTGACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTAAACATGTCTACAACGTCCTGCGCCGGGAAGGCACGCAGaaa                                                    <  2:279557/146‑1 (MQ=255)                                                                                          ccGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGaaa                                                    >  2:589429/1‑149 (MQ=255)                                                                                          ccGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCCCCAGAAAGGCACGCCGaaa                                                    >  2:255630/1‑149 (MQ=255)                                                                                             aaTTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGc                                                 <  1:274530/149‑1 (MQ=255)                                                                                                      ccACGCCGCTGTTTACCACCGACTAATTACGCAGCGCCCATGGCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTCCGCCCACAAGCCCCGCAGACAAGCGTCATCCac                                        >  2:132174/1‑149 (MQ=255)                                                                                                      ccACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGac                                        <  1:344787/149‑1 (MQ=255)                                                                                                        aCGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGacac                                      <  1:226472/149‑1 (MQ=255)                                                                                                        aCGCCGCTGTTTACAACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTCGAGCGTTCCGCGCCATGAACCGCGCCGCTGCACCTGTCTACCACGTCCTGCGCCAAGAAGGCACGCCGAAACGCGTCATCCacac                                      >  2:204323/1‑149 (MQ=255)                                                                                                              cTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCa                                <  1:401714/149‑1 (MQ=255)                                                                                                               tGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGCAGGCACGCCGAGAAGCGTCATCGACACCcagcag                               >  1:767760/1‑149 (MQ=255)                                                                                                                 tttACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACCACGGCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCCTCGCCACCATGCCGAc                             >  2:95144/1‑149 (MQ=255)                                                                                                                      caccGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACCCCATGCAGACCCGCa                        >  1:880262/1‑149 (MQ=255)                                                                                                                      caccGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCAGCGCCATAAACCGCGCCGCTGAACATGTCTACAAAGTCCTGCGCCAGAAACGCACGCACAAAAGCGTCATCGACACCATGCAGACCCGCa                        >  2:456696/1‑149 (MQ=255)                                                                                                                       accGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCCGGAAGGCACGCAGAAAAGCGTCCTCGACACCATGCAGCCCCGCac                       >  2:280642/1‑148 (MQ=255)                                                                                                                       accGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCaa                       >  2:733819/1‑149 (MQ=255)                                                                                                                       accGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATCCAGACCCGCaa                       >  2:308872/1‑149 (MQ=255)                                                                                                                         cgacgaATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACg                     >  1:610972/1‑149 (MQ=255)                                                                                                                         cgacgaATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACg                     >  1:561973/1‑149 (MQ=255)                                                                                                                         cgacgaATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACg                     >  2:692583/1‑149 (MQ=255)                                                                                                                                ttACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGAGCTGTa              >  1:493122/1‑149 (MQ=255)                                                                                                                                     cAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGAGCTGTACGaaa         <  2:493122/149‑1 (MQ=255)                                                                                                                                        cgcCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGTGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCa      <  2:747712/149‑1 (MQ=255)                                                                                                                                          cgattgtgGCAATGGCGCGGTACCCACTTTCAGCGTTCGGCGCCATGAACCGCGCCGCTGAACATGTCTCCTACGTCCTTTGCGAGGAAGGCACGCAGAAAGGCGTCATCGACACCATGCAGTCTCGCATCGCGCTGTACGAAAGCATc    <  2:14726/145‑1 (MQ=255)                                                                                                                                           ccATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCACCGAGCTGTAAGAACGCATCa   >  1:384735/1‑149 (MQ=255)                                                                                                                                           ccATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCa   >  1:660009/1‑149 (MQ=255)                                                                                                                                            cATGTGGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGGGCTGTACGAAAGCATCaa  <  2:242332/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            CGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCTGTTTACCACCGACGAATTACGCAGCGCCCATGTCGCAATGGCGCTGTACCCACTTTCAGCGTTCCGCGCCATGAACCGCGCCGCTGAACATGTCTACAACGTCCTGCGCCAGGAAGGCACGCAGAAAAGCGTCATCGACACCATGCAGACCCGCAACGAGCTGTACGAAAGCATCAA  >  NC_000913_3_pae_tpiA/349227‑349514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.