New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1826225	26 (0.710)	4 (0.110)	4/272	4.8	13.4%	coding (699/969 nt)	astE	succinylglutamate desuccinylase
	?	NC_000913_3_pae_tpiA	= 1826230	27 (0.780)					coding (694/969 nt)	astE	succinylglutamate desuccinylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1826073‑1826225 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgcaATTGCTGCGCTGCTATCTGGTGAGAGTGTCGGTATCGTGAGAACACCGCCGCTCCGTTATCGGGTGGTTTCGCAAATTACTCGCCACTCGCCGTCCTTCGAAATGCATATGGCAAGTGACACGCTGAATTTTATGCCGTTTGAGAAAG  <  NC_000913_3_pae_tpiA/1826230‑1826083                                                                                                                                                                                                                                                                                                               AGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCcg                                                                                                                                                          >  1:370086/1‑147               CTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAATTGCTGCGC                                                                                                                                            >  1:76250/1‑149                                           CTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCA                                                                                                                                                      <  1:523347/111‑1                                           CTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCA                                                                                                                                                      >  2:523347/1‑111                                                    TGCAGTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAATTGCTGCGCTGCTATCTGGTGAGAGTGTCGGTATCGTGAGAACACC                                                                                                       <  2:76250/149‑1                                                         TTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAATTGCTGCGCTGCTATCTGGTGAGAGT                                                                                                                           <  2:538103/124‑1                                                         TTCGAAGGAAGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAATTGCTGCGCTGCTATCTGGTGAGAGT                                                                                                                           >  1:538103/1‑124                                                                                                                                                         AATTGCTGCGCTGCTATCTGGTGAGAGTGTCGGTATCGTGAGAACACCGCCGCTCCGTTATCGGGTGGTTTCGCAAATTACTCGCCACTCGCCGTCCTTCGAACTGCATATGGCAAGTGACACGCTGAATTTTATGCCGTTTGAGAAAG  >  2:413178/1‑149                                                                                                                                                                                                                                                                                                               AGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1826073‑1826225 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgcaATTGCTGCGCTGCTATCTGGTGAGAGTGTCGGTATCGTGAGAACACCGCCGCTCCGTTATCGGGTGGTTTCGCAAATTACTCGCCACTCGCCGTCCTTCGAAATGCATATGGCAAGTGACACGCTGAATTTTATGCCGTTTGAGAAAG  <  NC_000913_3_pae_tpiA/1826230‑1826083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.