New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1826225	26 (0.710)	4 (0.110)	4/272	4.8	13.4%	coding (699/969 nt)	astE	succinylglutamate desuccinylase
	?	NC_000913_3_pae_tpiA	= 1826230	27 (0.780)					coding (694/969 nt)	astE	succinylglutamate desuccinylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTCCTGATGGAACAC  >  NC_000913_3_pae_tpiA/1826083‑1826371                                                                                                                                                    |                                                                                                                                              cTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCAGTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAAtt                                                                                                                                              <  2:413178/149‑1 (MQ=255)        aaaCGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGCCACCCTCACCAGATAGCAGGGCAGCAATTCCGCTgg                                                                                                                                       >  2:86741/1‑149 (MQ=255)        aaaCGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg                                                                                                                                       >  1:522354/1‑149 (MQ=255)          aCGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCa                                                                                                                                     >  2:482499/1‑149 (MQ=255)                                        tatGCATTTCGAAGGACGGCGAGTGGGGAGTAATTTGCTAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGc                                                                                                       <  2:102179/149‑1 (MQ=255)                                         atGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGcc                                                                                                      <  1:506399/149‑1 (MQ=255)                                               ttCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCAGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAgg                                                                                                >  2:406222/1‑149 (MQ=255)                                                  gAAGGACGGCGAATGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAACGGGCa                                                                                             >  1:233542/1‑149 (MQ=255)                                                              gtggcgagtAATTTCCGAAACCACCCGATAACGGAGCGGCGGTGTGCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCaa                                                                                 <  2:372359/149‑1 (MQ=255)                                                                ggcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGt                                                                               <  2:43449/149‑1 (MQ=255)                                                                 gcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGtt                                                                              >  1:97288/1‑149 (MQ=255)                                                                 gcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCAGTGTTCTCACGATACCGCCAACCTCACCATATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGtt                                                                              >  1:605739/1‑149 (MQ=255)                                                                  cgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCGCAAAGGGCAACGCTTTGCCAAGTTc                                                                             <  2:522354/149‑1 (MQ=255)                                                                    agtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGCTAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCa                                                                           >  1:642490/1‑149 (MQ=255)                                                                                    accCGAGACTGGGGCGGCGGTGTTCTCACGATAACGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAgcgc                                                           <  2:115881/149‑1 (MQ=255)                                                                                      cctaTAACCGAGCGGGGGTGTTCTCACTATCCTGACCCTCTGACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAgcgcgc                                                         <  1:601745/146‑1 (MQ=255)                                                                                                aGCGGCCGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCt                                               <  2:14742/149‑1 (MQ=255)                                                                                                   ggcggTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTGTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGgc                                            <  2:280182/149‑1 (MQ=255)                                                                                                                             ctcACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCGAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAg                  <  1:560765/149‑1 (MQ=255)                                                                                                                                aCCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGtt               >  1:419584/1‑149 (MQ=255)                                                                                                                                 ccAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGACAGACTGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTc              >  1:602164/1‑149 (MQ=255)                                                                                                                                        gcagcgtagcAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTCCTGATgg       <  2:370086/149‑1 (MQ=255)                                                                                                                                          agccaagcaATTGCGCTGGCAGTTACTGCAAACTGGAGAAGATCGTTTTGCCCAAAGGACAACGCTTTGCCGAATTCCAACGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATCGTGAAACATATCACCAGGTTCCTCATGGaa     >  2:570426/6‑149 (MQ=255)                                                                                                                                           gcgcagcAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTCCTGATGGAac    >  2:436615/1‑149 (MQ=255)                                                                                                                                             gcaggcaTTGCGCTGGCAGTTACTGCAAACTGGAGAAGATCGGTTTGCCCAGAAGGCAACGCTTTGCCAAGGTCCCGCGTACAGGGCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTGAACGTACCAGCAGGTTCCTGATGGAacac  <  2:376137/143‑1 (MQ=255)                                                                                                                                             gcagcaATTGCGCTGGCATTTACTGTAAACTTGCGAAGATTGTTTTGGCCAAAGGGCAACGTTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAGTGTCTGGCGCGGAAATGGGTAAATGTACCACCAGGTTCCTGATGGAacac  <  2:376139/149‑1 (MQ=255)                                                                                                                                                   aTTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTgg                                             <  1:604510/100‑1 (MQ=255)                                                                                                                                                   aTTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTgg                                             >  2:604510/1‑100 (MQ=255)                                                                                                                                                    |                                                                                                                                              CTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTCCTGATGGAACAC  >  NC_000913_3_pae_tpiA/1826083‑1826371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.