New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 2588975 =31 (0.850)4 (0.110) 4/274 4.8 11.5% coding (1381/3114 nt) acrD aminoglycoside/multidrug efflux system
?NC_000913_3_pae_tpiA 2588990 = 32 (0.910)coding (1396/3114 nt) acrD aminoglycoside/multidrug efflux system
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAG  >  NC_000913_3_pae_tpiA/2588833‑2589122
                                                                                                                                              |                                                                                                                                                   
ggAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGcacca                                                                                                                                               <  1:546127/149‑1 (MQ=255)
          gAACGTATTATGAGGGAGGAAGGACTCACGCCTCGCGGAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCAt                                                                                                                                     <  2:151354/149‑1 (MQ=255)
                 ttatGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTCCGGCGGCACCACCCGTCCCATTCATCGc                                                                                                                              >  2:324787/1‑149 (MQ=255)
                                   tcactcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGATCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                            >  1:423963/1‑149 (MQ=255)
                                   tcactcCTCGCGAAGCCACACGTAAACCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                            <  1:524026/149‑1 (MQ=255)
                                         ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATc                                                                                                                                >  1:527865/1‑123 (MQ=255)
                                         ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATc                                                                                                                                <  2:527865/123‑1 (MQ=255)
                                         ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCTCCAGTTCTCTATTACCATTGTTgcg                                                                                                      >  1:634065/1‑149 (MQ=255)
                                         ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTgcg                                                                                                      >  1:597827/1‑149 (MQ=255)
                                               aaGCCACACGTTATTCGTTGGGGGAGATCCAGGGGGCCCGGGTCGGGATTGCGTTGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATg                                                                                                <  2:316360/149‑1 (MQ=255)
                                                 gCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTACGATGGTTCTTTCGACGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGt                                                                                              >  1:567266/1‑149 (MQ=255)
                                                        gTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGTTGTCa                                                                                       >  2:532676/1‑149 (MQ=255)
                                                            aTCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGTTGCCATCTCTCCCCAGCTCTCTAGTACCATTGTTGCGGCGATGGTGCTGTCAGTAc                                                                                   <  2:521879/149‑1 (MQ=255)
                                                                 tGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCATCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                            <  1:435878/119‑1 (MQ=255)
                                                                 tGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCATCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                            >  2:435878/1‑119 (MQ=255)
                                                                     gCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTTGGCGGCACCACCGGTGCCATCTATCGCCAGTCCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCga                                                                          >  1:492455/1‑149 (MQ=255)
                                                                         aTCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCgatgat                                                                      >  1:456492/1‑149 (MQ=255)
                                                                               ggggCACTGGTCGCGCTCGCGATGGTGCTTTCGGCGGTATTTGTACCAATCGCCTTCTTAGGCGGCACCTCCGGTGCCAGCTAACGCCAGTTCTCTATTACCATTGTAGCGGCGATGGTACTGTCAGTCCTGGTAGCGTAGATCCTctc                                                                >  1:528312/1‑147 (MQ=25)
                                                                                 ggCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGGTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTc                                                              <  1:609648/149‑1 (MQ=255)
                                                                                  gCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTcc                                                             <  2:86489/149‑1 (MQ=255)
                                                                                    aCTGGGCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGTTAGCGGTGATCCTCATTCCgg                                                           >  2:494830/1‑149 (MQ=255)
                                                                                          cGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCtgt                                                     <  1:348130/149‑1 (MQ=255)
                                                                                                   gATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCacac                                            >  1:58481/1‑149 (MQ=255)
                                                                                                           tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCCTCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTgg                                                                                <  2:642294/105‑1 (MQ=255)
                                                                                                           tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTgg                                                                                >  1:642294/1‑105 (MQ=255)
                                                                                                           tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGGTGATCCTCACTCCGGCTCTTTGTGCCACACTCCTTAAg                                    >  1:258639/1‑149 (MQ=255)
                                                                                                                gcggTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACt                               >  2:549665/1‑149 (MQ=255)
                                                                                                                         gTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAgg                      <  1:642770/149‑1 (MQ=255)
                                                                                                                          tACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACATTACTAGAGCCACTGAAAAAAGGt                     >  1:346792/1‑149 (MQ=255)
                                                                                                                             cAATTGCCTTCTTCTGCGGCACCACCGGTGCCATCGATCGCCAGGTCTCTATTCTCATTGTTGCGGCTATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAg                  <  1:150367/149‑1 (MQ=255)
                                                                                                                                             cggcACCACCGGTGCAATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAg  <  1:324787/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAG  >  NC_000913_3_pae_tpiA/2588833‑2589122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.