New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	2588975 =	31 (0.850)	4 (0.110)	4/274	4.8	11.5%	coding (1381/3114 nt)	acrD	aminoglycoside/multidrug efflux system
	?	NC_000913_3_pae_tpiA	2588990 =	32 (0.910)					coding (1396/3114 nt)	acrD	aminoglycoside/multidrug efflux system
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTT  >  NC_000913_3_pae_tpiA/2588843‑2589130                                                                                                                                                    |                                                                                                                                             gAACGTATTATGAGGGAGGAAGGACTCACGCCTCGCGGAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCAt                                                                                                                                             <  2:151354/149‑1 (MQ=255)        ttatGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTCCGGCGGCACCACCCGTCCCATTCATCGc                                                                                                                                      >  2:324787/1‑149 (MQ=255)                          tcactcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGATCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                                    >  1:423963/1‑149 (MQ=255)                          tcactcCTCGCGAAGCCACACGTAAACCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                                    <  1:524026/149‑1 (MQ=255)                                ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATc                                                                                                                                        <  2:527865/123‑1 (MQ=255)                                ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATc                                                                                                                                        >  1:527865/1‑123 (MQ=255)                                ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCTCCAGTTCTCTATTACCATTGTTgcg                                                                                                              >  1:634065/1‑149 (MQ=255)                                ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTgcg                                                                                                              >  1:597827/1‑149 (MQ=255)                                      aaGCCACACGTTATTCGTTGGGGGAGATCCAGGGGGCCCGGGTCGGGATTGCGTTGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATg                                                                                                        <  2:316360/149‑1 (MQ=255)                                        gCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTACGATGGTTCTTTCGACGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGt                                                                                                      >  1:567266/1‑149 (MQ=255)                                               gTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGTTGTCa                                                                                               >  2:532676/1‑149 (MQ=255)                                                   aTCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGTTGCCATCTCTCCCCAGCTCTCTAGTACCATTGTTGCGGCGATGGTGCTGTCAGTAc                                                                                           <  2:521879/149‑1 (MQ=255)                                                        tGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCATCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                                    <  1:435878/119‑1 (MQ=255)                                                        tGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCATCACCGGTGCCATCTATCGCCAGTTCTCTATTACCAtt                                                                                                                    >  2:435878/1‑119 (MQ=255)                                                            gCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTTGGCGGCACCACCGGTGCCATCTATCGCCAGTCCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCga                                                                                  >  1:492455/1‑149 (MQ=255)                                                                aTCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCgatgat                                                                              >  1:456492/1‑149 (MQ=255)                                                                      ggggCACTGGTCGCGCTCGCGATGGTGCTTTCGGCGGTATTTGTACCAATCGCCTTCTTAGGCGGCACCTCCGGTGCCAGCTAACGCCAGTTCTCTATTACCATTGTAGCGGCGATGGTACTGTCAGTCCTGGTAGCGTAGATCCTctc                                                                        >  1:528312/1‑147 (MQ=25)                                                                        ggCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGGTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTc                                                                      <  1:609648/149‑1 (MQ=255)                                                                         gCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTcc                                                                     <  2:86489/149‑1 (MQ=255)                                                                           aCTGGGCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGTTAGCGGTGATCCTCATTCCgg                                                                   >  2:494830/1‑149 (MQ=255)                                                                                 cGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCtgt                                                             <  1:348130/149‑1 (MQ=255)                                                                                          gATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCacac                                                    >  1:58481/1‑149 (MQ=255)                                                                                                  tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCCTCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTgg                                                                                        <  2:642294/105‑1 (MQ=255)                                                                                                  tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTgg                                                                                        >  1:642294/1‑105 (MQ=255)                                                                                                  tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGGTGATCCTCACTCCGGCTCTTTGTGCCACACTCCTTAAg                                            >  1:258639/1‑149 (MQ=255)                                                                                                       gcggTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACt                                       >  2:549665/1‑149 (MQ=255)                                                                                                                gTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAgg                              <  1:642770/149‑1 (MQ=255)                                                                                                                 tACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACATTACTAGAGCCACTGAAAAAAGGt                             >  1:346792/1‑149 (MQ=255)                                                                                                                    cAATTGCCTTCTTCTGCGGCACCACCGGTGCCATCGATCGCCAGGTCTCTATTCTCATTGTTGCGGCTATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAg                          <  1:150367/149‑1 (MQ=255)                                                                                                                                    cggcACCACCGGTGCAATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAg          <  1:324787/149‑1 (MQ=255)                                                                                                                                          caccGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttt    >  1:30852/1‑149 (MQ=255)                                                                                                                                            ccGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttttt  <  2:58481/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             GAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTT  >  NC_000913_3_pae_tpiA/2588843‑2589130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.