Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 53 [0] [0] 53 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)51 (1.340) 41/276 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299351‑1299644
                                                                                                                                                   |                                                                                                                                                  
ttctCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                                   <  2:641537‑M1/149‑2 (MQ=255)
            agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                       <  1:176750‑M1/149‑14 (MQ=255)
            agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                       <  1:460254‑M1/149‑14 (MQ=255)
                         gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAACTGCTAAataataatcaatttttaaattatttt                                                                                                                          >  2:645667‑M1/1‑123 (MQ=255)
                          aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGCCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGAGCTAAataataatcacttattaaattattgtg                                                                                                                         >  2:653281‑M1/1‑122 (MQ=255)
                               cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATACTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGGCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                    >  2:667307‑M1/1‑117 (MQ=255)
                                ttAAATGCTTCTCGTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTGTTAATTCCACTGCCTTTGGTAGATGAAAGTGCTAAataataatcaattgttaaattattttgcatttc                                                                                                                   >  2:680024‑M1/1‑116 (MQ=255)
                                     tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGGAAAGGGCCAAatactaatcaattgttaaattattttgcatttcccgac                                                                                                              >  2:146329‑M1/1‑111 (MQ=255)
                                                 ccATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  <  1:565147‑M1/149‑51 (MQ=255)
                                                  catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttacattattatgcatttcactactggaacgggaatc                                                                                                 >  2:298202‑M1/1‑98 (MQ=255)
                                                   atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                >  1:278426‑M1/1‑97 (MQ=255)
                                                        gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                           <  2:352520‑M1/149‑58 (MQ=255)
                                                          tATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaaga                                                                                         <  1:283694‑M1/149‑60 (MQ=255)
                                                          tATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgaatttcactactggaactgtaatcagaaaaga                                                                                         >  2:51048‑M1/1‑90 (MQ=255)
                                                              ttCGCCACATCATAATCCTGGGCCTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                     <  1:383329‑M1/149‑64 (MQ=255)
                                                                 gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  <  2:531112‑M1/149‑67 (MQ=255)
                                                                 gCCACATCATAATACTGGGCTTGCTGAAGAATAATTGAAATGATATTATTATTTCGACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  <  1:334967‑M1/149‑67 (MQ=255)
                                                                      atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             >  1:252750‑M1/1‑78 (MQ=255)
                                                                        catAATTCTGGTGTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAGTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                           <  2:269349‑M1/149‑74 (MQ=255)
                                                                             tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatccattctcaaattatcctgaaagtcacttctggaactgtaatcagaaaagatagacatgcttagccaatc                                                                      <  1:512284‑M1/149‑79 (MQ=255)
                                                                              ccTGTGCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGCGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactacttgaactgtaatcagaaaagatagacatgcttagccaatct                                                                     <  1:108465‑M1/149‑80 (MQ=255)
                                                                                tGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctc                                                                    >  1:502311‑M1/1‑68 (MQ=255)
                                                                                tGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctc                                                                    <  2:502311‑M1/148‑81 (MQ=255)
                                                                                tGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataaacatgcttagccaatctct                                                                   >  2:548262‑M1/1‑68 (MQ=255)
                                                                                   gCTTGCGGAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGGGCTAAacaataatcaattgttaaatgattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                                <  2:47587‑M1/149‑85 (MQ=255)
                                                                                        cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           <  1:261731‑M1/149‑90 (MQ=255)
                                                                                        cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           <  1:261426‑M1/149‑90 (MQ=255)
                                                                                           aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                        >  1:357231‑M1/1‑57 (MQ=255)
                                                                                           aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                        <  2:357231‑M1/117‑61 (MQ=255)
                                                                                            agaaTAATTAAAATGATATTATTAATTCCAATTCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat                                                       <  1:120596‑M1/149‑94 (MQ=255)
                                                                                                     gAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatggacatgcttatccaatctctatttgattgaattgaaagatg                                              >  1:538566‑M1/1‑47 (MQ=255)
                                                                                                        ctgATATCATTAATTCCATCGTCATTGGTAAAAGAAAGTGCTTAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  1:90979‑M1/148‑106 (MQ=255)
                                                                                                        aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  1:421480‑M1/149‑106 (MQ=255)
                                                                                                        aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  2:314110‑M1/149‑106 (MQ=255)
                                                                                                              ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           >  1:357485‑M1/1‑38 (MQ=255)
                                                                                                              ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           <  2:357485‑M1/127‑90 (MQ=255)
                                                                                                              ttaataATTCCACTTCTTTTGGTAGAGGAAAGTGCGGCataagaattaacggttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                     <  1:144566‑M1/149‑112 (MQ=255)
                                                                                                               tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                           <  2:443023‑M1/78‑42 (MQ=255)
                                                                                                               tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                           >  1:443023‑M1/1‑37 (MQ=255)
                                                                                                                 ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggca                                  >  1:641117‑M1/1‑35 (MQ=255)
                                                                                                                          cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                         <  2:389730‑M1/149‑124 (MQ=255)
                                                                                                                             ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                      >  2:540064‑M1/1‑23 (MQ=255)
                                                                                                                               tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    >  2:187439‑M1/1‑21 (MQ=255)
                                                                                                                                    tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                           <  1:600361‑M1/73‑58 (MQ=255)
                                                                                                                                    tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                           >  2:600361‑M1/1‑16 (MQ=255)
                                                                                                                                       agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                                   >  1:660248‑M1/1‑13 (MQ=255)
                                                                                                                                       agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                                   <  2:660248‑M1/94‑82 (MQ=255)
                                                                                                                                       agGAAAGTGCTAAataataatcaagtgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctacttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctg            >  1:277173‑M1/1‑13 (MQ=255)
                                                                                                                                            aGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg       <  2:252750‑M1/149‑142 (MQ=255)
                                                                                                                                              tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt     <  1:571495‑M1/149‑144 (MQ=255)
                                                                                                                                              tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatggaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt     <  1:483037‑M1/149‑144 (MQ=255)
                                                                                                                                                cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtca   >  2:625998‑M1/1‑4 (MQ=255)
                                                                                                                                                 tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa  <  2:278426‑M1/149‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299351‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.