Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 53 [0] [0] 53 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)51 (1.340) 41/276 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843
                                                                                                                                                    |                                                                                                                                                 
ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                   <  2:641537‑M2/1‑1 (MQ=255)
            agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                       <  1:176750‑M2/13‑1 (MQ=255)
            agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                       <  1:460254‑M2/13‑1 (MQ=255)
                         gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaactgctaaATAATAATCAATTTTTaaattatttt                                                                                                                          >  2:645667‑M2/124‑147 (MQ=255)
                          atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctggccttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagagctaaATAATAATCACTTATTAAATTATtgtg                                                                                                                         >  2:653281‑M2/123‑149 (MQ=255)
                               cttaactgcttctcctcaccatcatgttattttcgccacatcatactcctgggcttgctgaagaataattgaaatgatattattaattccactggcttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                    >  2:667307‑M2/118‑149 (MQ=255)
                                ttaaatgcttctcgtcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattgttaattccactgcctttggtagatgaaagtgctaaATAATAATCAATTGTTAAATTATTTTGCATTTc                                                                                                                   >  2:680024‑M2/117‑149 (MQ=255)
                                     tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgctagaggaaagggccaaATACTAATCAATTGTTAAATTATTTTGcatttcccgac                                                                                                              >  2:146329‑M2/112‑144 (MQ=255)
                                                 ccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                  <  1:565147‑M2/50‑1 (MQ=255)
                                                  catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTACATTATTATGCATTTCACTACTGGAACGGGAATc                                                                                                 >  2:298202‑M2/99‑149 (MQ=255)
                                                   atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                                >  1:278426‑M2/98‑149 (MQ=255)
                                                        gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                           <  2:352520‑M2/57‑1 (MQ=255)
                                                          tattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                         <  1:283694‑M2/59‑1 (MQ=255)
                                                          tattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                         >  2:51048‑M2/91‑149 (MQ=255)
                                                              ttcgccacatcataatcctgggcctgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                     <  1:383329‑M2/63‑1 (MQ=255)
                                                                 gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                  <  2:531112‑M2/66‑1 (MQ=255)
                                                                 gccacatcataatactgggcttgctgaagaataattgaaatgatattattatttcgactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                  <  1:334967‑M2/66‑1 (MQ=255)
                                                                      atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                             >  1:252750‑M2/79‑149 (MQ=255)
                                                                        cataattctggtgttgctgaagaataattgaaatgatattattaattccagtgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                           <  2:269349‑M2/73‑1 (MQ=255)
                                                                             tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCCATTCTCAAATTATCCTGAAAGTCACTTCTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                      <  1:512284‑M2/78‑1 (MQ=255)
                                                                              cctgtgcgtgctgaagaataattgaaatgatattattaattccactgcctttggtagcggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTTGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtct                                                                     <  1:108465‑M2/79‑1 (MQ=255)
                                                                                tgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtctc                                                                    >  1:502311‑M2/69‑148 (MQ=255)
                                                                                tgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtctc                                                                    <  2:502311‑M2/80‑1 (MQ=255)
                                                                                tgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAAACATGCTTAGCCAATctct                                                                   >  2:548262‑M2/69‑149 (MQ=255)
                                                                                   gcttgcggaagaataattgaaattatattattaattccactgcctttggtagaggaaagggctaaACAATAATCAATTGTTAAATGATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                                <  2:47587‑M2/84‑1 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:261731‑M2/89‑1 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  1:261426‑M2/89‑1 (MQ=255)
                                                                                           aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                        >  1:357231‑M2/58‑117 (MQ=255)
                                                                                           aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                        <  2:357231‑M2/60‑1 (MQ=255)
                                                                                            agaataattaaaatgatattattaattccaattcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                       <  1:120596‑M2/93‑1 (MQ=255)
                                                                                                     gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATGGACATGCTTATCCAATCTCTATTTGATTGAATTGAAAGAtg                                              >  1:538566‑M2/48‑149 (MQ=255)
                                                                                                        ctgatatcattaattccatcgtcattggtaaaagaaagtgcttaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                           <  1:90979‑M2/105‑1 (MQ=255)
                                                                                                        atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                           <  1:421480‑M2/105‑1 (MQ=255)
                                                                                                        atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                           <  2:314110‑M2/105‑1 (MQ=255)
                                                                                                              ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           >  1:357485‑M2/39‑127 (MQ=255)
                                                                                                              ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  2:357485‑M2/89‑1 (MQ=255)
                                                                                                              ttaataattccacttcttttggtagaggaaagtgcggcATAAGAATTAACGGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                     <  1:144566‑M2/111‑1 (MQ=255)
                                                                                                               tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                           <  2:443023‑M2/41‑1 (MQ=255)
                                                                                                               tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                           >  1:443023‑M2/38‑78 (MQ=255)
                                                                                                                 ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                                  >  1:641117‑M2/36‑149 (MQ=255)
                                                                                                                          ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                         <  2:389730‑M2/123‑1 (MQ=255)
                                                                                                                             cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                      >  2:540064‑M2/24‑149 (MQ=255)
                                                                                                                               tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                    >  2:187439‑M2/22‑149 (MQ=255)
                                                                                                                                    tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                           <  1:600361‑M2/57‑1 (MQ=255)
                                                                                                                                    tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                           >  2:600361‑M2/17‑73 (MQ=255)
                                                                                                                                       aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                   >  1:660248‑M2/14‑94 (MQ=255)
                                                                                                                                       aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                   <  2:660248‑M2/81‑1 (MQ=255)
                                                                                                                                       aggaaagtgctaaATAATAATCAAGTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTACTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTg            >  1:277173‑M2/14‑149 (MQ=255)
                                                                                                                                            agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg       <  2:252750‑M2/141‑1 (MQ=255)
                                                                                                                                              tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt     <  1:571495‑M2/143‑1 (MQ=255)
                                                                                                                                              tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATGGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt     <  1:483037‑M2/143‑1 (MQ=255)
                                                                                                                                                ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa   >  2:625998‑M2/5‑149 (MQ=255)
                                                                                                                                                 taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCaa  <  2:278426‑M2/146‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.