New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3357043	49 (1.680)	3 (0.110)	3/266	4.0	6.3%	coding (2319/4461 nt)	gltB	glutamate synthase, large subunit
	?	NC_000913_3_pae_tpiA	= 3357057	43 (1.560)					coding (2333/4461 nt)	gltB	glutamate synthase, large subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGG  >  NC_000913_3_pae_tpiA/3356903‑3357188                                                                                                                                             |                                                                                                                                                  tgctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTaa                                                                                                                                           <  2:277086/149‑1 (MQ=255) tgctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTaa                                                                                                                                           >  1:264356/1‑149 (MQ=255)      gAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCa                                                                                                                                      <  2:294740/149‑1 (MQ=255)        aaCTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATc                                                                                                                                    <  1:95338/149‑1 (MQ=255)        aaCTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCCTTGAAGCCTCCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATc                                                                                                                                    <  2:85788/149‑1 (MQ=255)          cTGTTTGAAGCGGGCGGGCTACACGATGGTGCGGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCGTTGGTGGAGGAAGGTTTGAAGACTTCCAGCAGGATGTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCcagcag                                                                                                                                  <  2:291676/149‑1 (MQ=255)           tGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGc                                                                                                                                 >  1:338981/1‑149 (MQ=255)               tGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAgg                                                                                                                             <  2:199794/149‑1 (MQ=255)                  aGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCg                                                                                                                          <  1:183360/149‑1 (MQ=255)                      gtcggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTct                                                                                                                      >  1:451665/1‑149 (MQ=255)                             tACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGCGCAAGCTTTGAAGACTTCCAGCAGGATCTGCGGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGaaa                                                                                                               >  1:103486/1‑149 (MQ=255)                             tACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGaaa                                                                                                               <  2:451665/149‑1 (MQ=255)                               cacGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTaa                                                                                                                                           <  2:498604/119‑1 (MQ=255)                               cacGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTaa                                                                                                                                           >  1:498604/1‑119 (MQ=255)                                acGATGATGGAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATAc                                                                                                            <  2:113924/149‑1 (MQ=255)                                  gatgatGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGt                                                                                                          >  2:481518/1‑149 (MQ=255)                                  gatgatGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGt                                                                                                          >  1:268907/1‑149 (MQ=255)                                     gatgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCa                                                                                                       >  1:318854/1‑149 (MQ=255)                                          aGTGGGCCTGTGCTTCCAGGGAGCGGTCAGGCGCATTGGTGGAGCAAGGTTTGAAGACTTCCAGCAGGATCTGCTGAATGTGTCGAAACGTGCCTGGCGTGCGCGTAAGCCCATCAGCCAGCGCCAGCTGATGAAATACGTCCAcggcg                                                                                                  >  2:505784/1‑149 (MQ=255)                                               gCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGaa                                                                                             <  2:460751/149‑1 (MQ=255)                                                  tgtgCTTCCAGGGGGCGGTTAGCCGCATTGGTGGAACAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATAc                                                                                          >  2:434121/1‑149 (MQ=255)                                                  tgtgCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCCGCGCATAc                                                                                          >  2:455315/1‑149 (MQ=255)                                                         ccAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGGCCATGAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCt                                                                                   >  1:98148/1‑149 (MQ=255)                                                          cAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTa                                                                                  <  1:290316/149‑1 (MQ=255)                                                             ggggCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCCCGGCGGCGAATACCACGCCTACaa                                                                               >  2:444229/1‑149 (MQ=255)                                                               ggCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAAcc                                                                             <  1:263573/149‑1 (MQ=255)                                                               ggCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGCCGCATACCCCGCCTACAAcc                                                                             >  2:84195/1‑149 (MQ=255)                                                                         cGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACgtggt                                                                   >  2:310172/1‑149 (MQ=255)                                                                               ggtggAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGCATACCACGCCTACAACCCGGACGTGGTGCGCAc                                                             >  2:437110/1‑149 (MQ=255)                                                                                      cAAGGTTTGAAGACTTCCAGCAGGAAGTGCTGAGGCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCGGAAATCAGTCCACGGCGGCGAATACCACGCCTACAACCCGAACGTGGAGCGCCCGCTCcaa                                                      >  2:393634/1‑149 (MQ=255)                                                                                       aaGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGGCCGCTGcacc                                                     >  2:173343/1‑147 (MQ=255)                                                                                           tttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCAc                                                                                       <  1:58605/111‑1 (MQ=255)                                                                                           tttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCAc                                                                                       >  2:58605/1‑111 (MQ=255)                                                                                           tttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCAGGCTGCAACAAGc                                                 >  1:168749/1‑149 (MQ=255)                                                                                               aaGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTa                                             <  2:382007/149‑1 (MQ=255)                                                                                                    ttCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAg                                        >  2:12134/1‑149 (MQ=255)                                                                                                     tCCAACAGGACCTGCATAATCATTAGAAACGTACCTGCCCGTTGCGTAGCCCCATCGGCCAGGTCCGCCTGCCGAAACACCTCCACGGCGGCGAACACCACGCCTACAACCCGGACGTCGTGCGCACGCTGCAACAAGCGGTACAAAgc                                       <  2:54721/149‑1 (MQ=255)                                                                                                        agcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAgcggc                                    <  1:215785/149‑1 (MQ=255)                                                                                                        agcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAgcggc                                    >  2:72261/1‑149 (MQ=255)                                                                                                          cagGATCTGCGGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGa                                  <  2:327487/149‑1 (MQ=255)                                                                                                             gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAgga                               >  1:82448/1‑147 (MQ=255)                                                                                                             gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTa                               >  1:158810/1‑149 (MQ=255)                                                                                                             gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGCCGAATACCACGCCTACAACCCGGACGTGGTGCGCACCCTGCAACAACCGGAACAAAGCGGCGAgaa                               >  2:174604/1‑147 (MQ=255)                                                                                                             gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGAGCGTATCCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTa                               <  1:481518/149‑1 (MQ=255)                                                                                                                   ctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGa                         >  1:131211/1‑149 (MQ=255)                                                                                                                           gTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAgg                 >  2:230499/1‑149 (MQ=255)                                                                                                                                     gCCTGGCTGGTGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAAACAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGaa       <  2:217282/149‑1 (MQ=255)                                                                                                                                         ggctggcGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTg   <  2:131211/149‑1 (MQ=255)                                                                                                                                          gctggcGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTgg  <  2:158810/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  TGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGG  >  NC_000913_3_pae_tpiA/3356903‑3357188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.