New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3357043	49 (1.680)	3 (0.110)	3/266	4.0	6.3%	coding (2319/4461 nt)	gltB	glutamate synthase, large subunit
	?	NC_000913_3_pae_tpiA	= 3357057	43 (1.560)					coding (2333/4461 nt)	gltB	glutamate synthase, large subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATG  >  NC_000913_3_pae_tpiA/3356910‑3357194                                                                                                                                                    |                                                                                                                                          aaCTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATc                                                                                                                                          <  1:95338/149‑1 (MQ=255) aaCTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCCTTGAAGCCTCCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATc                                                                                                                                          <  2:85788/149‑1 (MQ=255)   cTGTTTGAAGCGGGCGGGCTACACGATGGTGCGGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCGTTGGTGGAGGAAGGTTTGAAGACTTCCAGCAGGATGTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCcagcag                                                                                                                                        <  2:291676/149‑1 (MQ=255)    tGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGc                                                                                                                                       >  1:338981/1‑149 (MQ=255)        tGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAgg                                                                                                                                   <  2:199794/149‑1 (MQ=255)           aGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCg                                                                                                                                <  1:183360/149‑1 (MQ=255)               gtcggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTct                                                                                                                            >  1:451665/1‑149 (MQ=255)                      tACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGCGCAAGCTTTGAAGACTTCCAGCAGGATCTGCGGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGaaa                                                                                                                     >  1:103486/1‑149 (MQ=255)                      tACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGaaa                                                                                                                     <  2:451665/149‑1 (MQ=255)                         acGATGATGGAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATAc                                                                                                                  <  2:113924/149‑1 (MQ=255)                           gatgatGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGt                                                                                                                >  1:268907/1‑149 (MQ=255)                           gatgatGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGt                                                                                                                >  2:481518/1‑149 (MQ=255)                              gatgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCa                                                                                                             >  1:318854/1‑149 (MQ=255)                                   aGTGGGCCTGTGCTTCCAGGGAGCGGTCAGGCGCATTGGTGGAGCAAGGTTTGAAGACTTCCAGCAGGATCTGCTGAATGTGTCGAAACGTGCCTGGCGTGCGCGTAAGCCCATCAGCCAGCGCCAGCTGATGAAATACGTCCAcggcg                                                                                                        >  2:505784/1‑149 (MQ=255)                                        gCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGaa                                                                                                   <  2:460751/149‑1 (MQ=255)                                           tgtgCTTCCAGGGGGCGGTTAGCCGCATTGGTGGAACAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATAc                                                                                                >  2:434121/1‑149 (MQ=255)                                           tgtgCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCCGCGCATAc                                                                                                >  2:455315/1‑149 (MQ=255)                                                  ccAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGGCCATGAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCt                                                                                         >  1:98148/1‑149 (MQ=255)                                                   cAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTa                                                                                        <  1:290316/149‑1 (MQ=255)                                                      ggggCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCCCGGCGGCGAATACCACGCCTACaa                                                                                     >  2:444229/1‑149 (MQ=255)                                                        ggCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAAcc                                                                                   <  1:263573/149‑1 (MQ=255)                                                        ggCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGCCGCATACCCCGCCTACAAcc                                                                                   >  2:84195/1‑149 (MQ=255)                                                                  cGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACgtggt                                                                         >  2:310172/1‑149 (MQ=255)                                                                        ggtggAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGCATACCACGCCTACAACCCGGACGTGGTGCGCAc                                                                   >  2:437110/1‑149 (MQ=255)                                                                               cAAGGTTTGAAGACTTCCAGCAGGAAGTGCTGAGGCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCGGAAATCAGTCCACGGCGGCGAATACCACGCCTACAACCCGAACGTGGAGCGCCCGCTCcaa                                                            >  2:393634/1‑149 (MQ=255)                                                                                aaGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGGCCGCTGcacc                                                           >  2:173343/1‑147 (MQ=255)                                                                                    tttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCAc                                                                                             >  2:58605/1‑111 (MQ=255)                                                                                    tttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCAc                                                                                             <  1:58605/111‑1 (MQ=255)                                                                                    tttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCAGGCTGCAACAAGc                                                       >  1:168749/1‑149 (MQ=255)                                                                                        aaGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTa                                                   <  2:382007/149‑1 (MQ=255)                                                                                             ttCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAg                                              >  2:12134/1‑149 (MQ=255)                                                                                              tCCAACAGGACCTGCATAATCATTAGAAACGTACCTGCCCGTTGCGTAGCCCCATCGGCCAGGTCCGCCTGCCGAAACACCTCCACGGCGGCGAACACCACGCCTACAACCCGGACGTCGTGCGCACGCTGCAACAAGCGGTACAAAgc                                             <  2:54721/149‑1 (MQ=255)                                                                                                 agcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAgcggc                                          <  1:215785/149‑1 (MQ=255)                                                                                                 agcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAgcggc                                          >  2:72261/1‑149 (MQ=255)                                                                                                   cagGATCTGCGGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGa                                        <  2:327487/149‑1 (MQ=255)                                                                                                      gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAgga                                     >  1:82448/1‑147 (MQ=255)                                                                                                      gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTa                                     >  1:158810/1‑149 (MQ=255)                                                                                                      gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGCCGAATACCACGCCTACAACCCGGACGTGGTGCGCACCCTGCAACAACCGGAACAAAGCGGCGAgaa                                     >  2:174604/1‑147 (MQ=255)                                                                                                      gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGAGCGTATCCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTa                                     <  1:481518/149‑1 (MQ=255)                                                                                                            ctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGa                               >  1:131211/1‑149 (MQ=255)                                                                                                                    gTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAgg                       >  2:230499/1‑149 (MQ=255)                                                                                                                              gCCTGGCTGGTGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAAACAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGaa             <  2:217282/149‑1 (MQ=255)                                                                                                                                  ggctggcGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTg         <  2:131211/149‑1 (MQ=255)                                                                                                                                   gctggcGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTgg        <  2:158810/149‑1 (MQ=255)                                                                                                                                        cgcgTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAAt   <  2:264356/149‑1 (MQ=255)                                                                                                                                         gcgTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGACGGAGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATAGGCGAAGCTGGTTAATg  >  2:216700/1‑149 (MQ=255)                                                                                                                                                  cATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCgg                                                     >  2:286946/1‑89 (MQ=255)                                                                                                                                                  cATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCgg                                                     <  1:286946/89‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                          AACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATG  >  NC_000913_3_pae_tpiA/3356910‑3357194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.