New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3563404 =	29 (0.990)	4 (0.140)	4/268	3.6	11.7%	coding (1392/1506 nt)	glpD	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
	?	NC_000913_3_pae_tpiA	3563422 =	33 (1.190)					coding (1410/1506 nt)	glpD	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAA  >  NC_000913_3_pae_tpiA/3563266‑3563549                                                                                                                                           |                                                                                                                                                  tCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCtgt                                                                                                                                         >  1:307800/1‑149 (MQ=255)  cGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCtgtg                                                                                                                                        <  2:264856/149‑1 (MQ=255)    cACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGc                                                                                                                                      <  1:44646/149‑1 (MQ=255)                   cAGCGAGCTGCTACACGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAg                                                                                                                       >  2:320936/1‑149 (MQ=255)                         gctgctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAtgt                                                                                                                 <  1:413350/149‑1 (MQ=255)                         gctgctgccAGGCAATGGGGTAACGGTAAGCGACCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAtgt                                                                                                                 <  1:67199/149‑1 (MQ=255)                          ctgctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAtgtg                                                                                                                >  2:10765/1‑149 (MQ=255)                           tgctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTgg                                                                                                               >  1:416761/1‑149 (MQ=255)                            gctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGc                                                                                                              <  2:510916/149‑1 (MQ=255)                               gctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACACCCGCTGGCGTCGCACAAAACAAGGCATGTGGCTac                                                                                                           >  1:50578/1‑148 (MQ=255)                                   ggCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGc                                                                                                       <  1:498934/149‑1 (MQ=255)                                      aaTGAGGGAAAGGGAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGa                                                                                                    <  1:416131/149‑1 (MQ=255)                                       aTGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGAt                                                                                                   <  1:10765/149‑1 (MQ=255)                                        tGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATc                                                                                                  <  1:467960/149‑1 (MQ=255)                                                 gggcaGCGAACGCGCGGAAGGTTTCGGTCATGAGGTCCACGAAGCGGAGCTGACAGACCTGGTGGATCACGAATGGGGCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAAtctc                                                                                         <  1:85560/145‑1 (MQ=255)                                                   caaGCGATCTCGGGGGAGATTTCGGGCATGAGCTCTACGAAGCGGAGCTGAACTACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGCGGCGTCGCACAAAACACGGCATGTGGCTAAATGCGGATCAACAATCTCgt                                                                                       <  2:166846/148‑1 (MQ=255)                                                                         cGGTCATGGGTTGTGCGAGGCGGAGCTGAAATCCCTGGTGGCCCACGAATGGGTCCGCCGCGCCGGCGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt                                                                 <  1:97386/149‑1 (MQ=255)                                                                              aTGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGGGGGTCAGTGGCTGGGGGAGCATTCg                                                            >  1:240388/1‑149 (MQ=255)                                                                               tGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgc                                                           >  1:242401/1‑149 (MQ=255)                                                                                   ttCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagca                                                       >  2:140633/1‑149 (MQ=255)                                                                                      tgcGAAGCGGGGCTGCAATGCCTGGGTGATCACGGACGGGTCCGCCGCGCCGAAGACCCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCagag                                                    <  1:172303/147‑1 (MQ=255)                                                                                      tACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCagag                                                    <  2:307800/149‑1 (MQ=255)                                                                                                       aTACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGcgtcgt                                   <  1:262639/149‑1 (MQ=255)                                                                                                         aCCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTaa                                 >  1:333371/1‑149 (MQ=255)                                                                                                           cTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAAt                               >  1:134115/1‑149 (MQ=255)                                                                                                           cTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAAt                               <  1:243616/149‑1 (MQ=255)                                                                                                                   tCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGGCTGTCTTTTAGTTGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTaa                       >  1:503561/1‑149 (MQ=255)                                                                                                                       gAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGAGGAGTATACGCAGCAGAGGTTATCGTTGGCGACGTAAATTAACGTAAGGTg                   >  1:489302/1‑149 (MQ=255)                                                                                                                                        cgacgaCGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCaa  >  2:451272/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                  TCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAA  >  NC_000913_3_pae_tpiA/3563266‑3563549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.