New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3563404 =	29 (0.990)	4 (0.140)	4/268	3.6	11.7%	coding (1392/1506 nt)	glpD	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
	?	NC_000913_3_pae_tpiA	3563422 =	33 (1.190)					coding (1410/1506 nt)	glpD	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGA  >  NC_000913_3_pae_tpiA/3563284‑3563568                                                                                                                                           |                                                                                                                                                   cAGCGAGCTGCTACACGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAg                                                                                                                                          >  2:320936/1‑149 (MQ=255)       gctgctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAtgt                                                                                                                                    <  1:413350/149‑1 (MQ=255)       gctgctgccAGGCAATGGGGTAACGGTAAGCGACCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAtgt                                                                                                                                    <  1:67199/149‑1 (MQ=255)        ctgctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAtgtg                                                                                                                                   >  2:10765/1‑149 (MQ=255)         tgctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTgg                                                                                                                                  >  1:416761/1‑149 (MQ=255)          gctgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGc                                                                                                                                 <  2:510916/149‑1 (MQ=255)             gctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACACCCGCTGGCGTCGCACAAAACAAGGCATGTGGCTac                                                                                                                              >  1:50578/1‑148 (MQ=255)                 ggCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGc                                                                                                                          <  1:498934/149‑1 (MQ=255)                    aaTGAGGGAAAGGGAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGa                                                                                                                       <  1:416131/149‑1 (MQ=255)                     aTGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGAt                                                                                                                      <  1:10765/149‑1 (MQ=255)                      tGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATc                                                                                                                     <  1:467960/149‑1 (MQ=255)                               gggcaGCGAACGCGCGGAAGGTTTCGGTCATGAGGTCCACGAAGCGGAGCTGACAGACCTGGTGGATCACGAATGGGGCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAAtctc                                                                                                            <  1:85560/145‑1 (MQ=255)                                 caaGCGATCTCGGGGGAGATTTCGGGCATGAGCTCTACGAAGCGGAGCTGAACTACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGCGGCGTCGCACAAAACACGGCATGTGGCTAAATGCGGATCAACAATCTCgt                                                                                                          <  2:166846/148‑1 (MQ=255)                                                       cGGTCATGGGTTGTGCGAGGCGGAGCTGAAATCCCTGGTGGCCCACGAATGGGTCCGCCGCGCCGGCGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt                                                                                    <  1:97386/149‑1 (MQ=255)                                                            aTGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGGGGGTCAGTGGCTGGGGGAGCATTCg                                                                               >  1:240388/1‑149 (MQ=255)                                                             tGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgc                                                                              >  1:242401/1‑149 (MQ=255)                                                                 ttCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagca                                                                          >  2:140633/1‑149 (MQ=255)                                                                    tgcGAAGCGGGGCTGCAATGCCTGGGTGATCACGGACGGGTCCGCCGCGCCGAAGACCCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCagag                                                                       <  1:172303/147‑1 (MQ=255)                                                                    tACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCagag                                                                       <  2:307800/149‑1 (MQ=255)                                                                                     aTACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGcgtcgt                                                      <  1:262639/149‑1 (MQ=255)                                                                                       aCCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTaa                                                    >  1:333371/1‑149 (MQ=255)                                                                                         cTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAAt                                                  >  1:134115/1‑149 (MQ=255)                                                                                         cTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAAt                                                  <  1:243616/149‑1 (MQ=255)                                                                                                 tCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGGCTGTCTTTTAGTTGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTaa                                          >  1:503561/1‑149 (MQ=255)                                                                                                     gAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGAGGAGTATACGCAGCAGAGGTTATCGTTGGCGACGTAAATTAACGTAAGGTg                                      >  1:489302/1‑149 (MQ=255)                                                                                                                      cgacgaCGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCaa                     >  2:451272/1‑149 (MQ=255)                                                                                                                           acgCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCCGATCCGATTTCAATCTgg                >  2:467157/1‑149 (MQ=255)                                                                                                                              cccgggggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCGGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCt             <  1:284544/143‑1 (MQ=255)                                                                                                                               ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTg                 >  1:265777/1‑144 (MQ=255)                                                                                                                               ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTg                 <  2:265777/144‑1 (MQ=255)                                                                                                                                ttgtgGGGTCGGACAAGACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGGTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTga           <  2:484564/148‑1 (MQ=255)                                                                                                                                gtggggcggcGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTga           <  1:175918/145‑1 (MQ=255)                                                                                                                                cTGTGGGGTCGCACAAAACAAGGCATGTGGGTAAATGTGGATCAAAAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGTAGGAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTga           <  2:240388/149‑1 (MQ=255)                                                                                                                                cTGCGGCGGCGCACAAAACAAGGCACGTGGCTAAATGCGGAGCAACCATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTTAATAAAGCGAAATTGATCAGGTCAGATTTCAATCTGGCCTga           <  1:254590/149‑1 (MQ=255)                                                                                                                                 tgtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTgag          <  1:451272/149‑1 (MQ=255)                                                                                                                                    ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTg                                                             >  1:328810/1‑95 (MQ=255)                                                                                                                                    ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTg                                                             <  2:328810/95‑1 (MQ=255)                                                                                                                                       gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat    <  2:134115/149‑1 (MQ=255)                                                                                                                                         cccacaAAACAACGCATGGGGATAAATCCGGATCAACAATCTCGTCTGAGTCAGTGCCTGGTGGAGTATACGCAGCAGATGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgatga  <  1:514214/147‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                   CAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGA  >  NC_000913_3_pae_tpiA/3563284‑3563568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.