New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	1758437 =	16 (0.570)	5 (0.180)	5/272	3.0	23.6%	coding (289/1005 nt)	ldtE	murein L,D‑transpeptidase
	?	NC_000913_3_pae_tpiA	1758456 =	17 (0.620)					coding (270/1005 nt)	ldtE	murein L,D‑transpeptidase
Rejected: Coverage evenness skew score above cutoff.

GGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCC  >  NC_000913_3_pae_tpiA/1758299‑1758585                                                                                                                                           |                                                                                                                                                     ggATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGt                                                                                                                                            <  1:457931/149‑1 (MQ=255)     tttCTGCCCAACACGCGGTTCCATCACCGGTGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATCTTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCAt                                                                                                                                        <  1:208506/149‑1 (MQ=255)                cacGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAAGAAc                                                                                                                             >  1:358719/1‑149 (MQ=255)                    cgTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATAACTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGtt                                                                                                                         <  2:310072/149‑1 (MQ=255)                     gTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTtg                                                                                                                        <  2:277382/149‑1 (MQ=255)                         ccaccaCCGGCGTTTCCAGCCCCTGCAATCCAATACCTTTTGGATAAACCTGCACAATATTTTCGCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAACTAATCGGTTATCTCCTGACGCGGTGCATCAGGTAATAACAGTTGTGaa                                                                                                                    <  2:457916/149‑1 (MQ=255)                                            ccccTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCCGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTAc                                                                                                 >  2:259451/1‑149 (MQ=255)                                                    actcAAGACCTATTGGATAAACCTGCACAATATTTTCCGCCGGCGGATAATAATAAAGGCGCAGCGCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATACCAGTTGTGAAGGAATAGTTATCGTCGTACCAGGtttt                                                                                         <  2:293714/146‑1 (MQ=255)                                                            ccTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCgg                                                                                 <  1:236684/149‑1 (MQ=255)                                                              tATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGCTTTGGCACCgggg                                                                               >  2:240459/1‑149 (MQ=255)                                                                     tAAACCTGCACAATATTTGCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAgt                                                                        <  1:80712/149‑1 (MQ=255)                                                                         ccTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGttt                                                                    >  2:204346/1‑148 (MQ=255)                                                                                        ctcCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATc                                                     <  1:156592/149‑1 (MQ=255)                                                                                                            aGGCGCAGCGCTGCAAGGTTAACGATACTCCCCTGACGCGGTGCATCAGGTAATCACAGTTGTGAAGGAATAGTTATCGGCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCaaa                                 <  1:430051/149‑1 (MQ=255)                                                                                                                                aaCGATAATCCCCTGACGCGGTGCATCAGGTAATAATAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGaa             <  1:226649/149‑1 (MQ=255)                                                                                                                                   gATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCTGGCAATAGCCTGAAGGt          >  2:120393/1‑149 (MQ=255)                                                                                                                                           ccTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGCTTTTTATcc  >  2:310826/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                     GGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCC  >  NC_000913_3_pae_tpiA/1758299‑1758585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.