New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 2810870 =34 (0.930)5 (0.140)
+C
5/284 4.4 13.2% coding (101/531 nt) mprA transcriptional repressor of microcin B17 synthesis and multidrug efflux
?NC_000913_3_pae_tpiA 2810877 = 32 (0.880)coding (108/531 nt) mprA transcriptional repressor of microcin B17 synthesis and multidrug efflux
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCC  >  NC_000913_3_pae_tpiA/2810729‑2811021
                                                                                                                                                     |                                                                                                                                                
gTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                                                                                                  <  1:6679/149‑1 (MQ=255)
   gTTACTATATCGGCTGAAATTAATGAGGTCATCAAATTTGACATGGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATACACATGCaa                                                                                                                                               >  1:154293/1‑149 (MQ=255)
    ttACTATATCGGCTGAAATTAATGAGGTCATACACAACTGGATAGTTCGTTTACGTCCATTGAACAAATGCTAAAATTTCGCGCCAGCCTCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaaa                                                                                                                                              <  1:56065/149‑1 (MQ=255)
                     aTTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                                                                             <  1:201790/149‑1 (MQ=255)
                      ttAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                                                                                 >  2:394382/1‑144 (MQ=255)
                      ttAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                                                                                 <  1:394382/144‑1 (MQ=255)
                                taTACTCAAATGTATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCATACACGAAGATTTTTCTTATCAGGAGATCCTTCTGATTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAAt                                                                                                                  <  1:411473/148‑1 (MQ=255)
                                 ctACCCAAATTGATAGTTGGTTTACGCCCATTGAATAAATGCTAAAATTTCGCGCCAGCCGCCACTAAGATTTTCCTTATCAGGAGATCCTTCTGACACGTCTTTGCATGCACATGCATAGCAAGCTGCTGGAGAACCGCAATAAAATg                                                                                                                 <  2:469610/148‑1 (MQ=255)
                                 aTACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATg                                                                                                                 >  1:475863/1‑149 (MQ=255)
                                      cAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGaa                                                                                                            <  1:356157/149‑1 (MQ=255)
                                               tAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgg                                                                                                    <  2:475863/149‑1 (MQ=255)
                                                  ttCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAGTGCTCAGGGGa                                                                                                 >  1:41384/1‑149 (MQ=255)
                                                       ttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc                                                                                            <  1:130939/149‑1 (MQ=255)
                                                         aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAATGCTCAGGGGATTAACga                                                                                          <  2:76336/149‑1 (MQ=255)
                                                          cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag                                                                                         >  2:83085/1‑149 (MQ=255)
                                                           gCCCATTGAACAAATGCTAAAATTTCGCGTCAGCCGCCACGAAGAGTTTGCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGGACATGCAAAGCAAGGTGCTGGAGAACCGCAATAAAATGCTGAAGACTCAGGGGATTAACgaga                                                                                        >  2:583200/1‑149 (MQ=255)
                                                           gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga                                                                                        <  1:43869/149‑1 (MQ=255)
                                                                ttGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGACGGCTCAAGGGATTAAAGAGACggtg                                                                                   >  2:67381/1‑149 (MQ=255)
                                                                           cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCATTCTTACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                                                                        >  1:607979/1‑149 (MQ=255)
                                                                           cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTGATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATATAACGCTGAAGGCTCAGGGGATCAACGAGACGTTGTTTATGGCGtt                                                                        <  1:510536/149‑1 (MQ=255)
                                                                            tAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATggagcag                                                                       >  2:482839/1‑144 (MQ=255)
                                                                                     ccgcCTGCCGTCACTAATATTTTCCTTATCATGTGTTTCTTCTGATTTGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg                                                              <  1:24068/148‑1 (MQ=255)
                                                                                             cgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGTGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa                                                      <  1:580211/149‑1 (MQ=255)
                                                                                                               tttgagaagaTCCTGCGGACTCGTTTTAGCATGAACAGGCAAAGCAAGCTGTTGGAGAACCGCTATAAACTGTCGCTGGCCATGGGGATTAATCAGATGTTGTTCATGGCGTTGATTACGCTGGAGTCTCAGGAAAAACCCAGTATTCa                                    <  2:8328/142‑1 (MQ=255)
                                                                                                                aTCAGGAGATTCTTCTGACGCGTCTTTGCATGCACATGCAAAGCAAGCTGGTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGTGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAg                                   <  2:89473/149‑1 (MQ=255)
                                                                                                                  cAGGAGATCCTCCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGcc                                 <  2:633262/149‑1 (MQ=255)
                                                                                                                    ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCtt                               >  1:127407/1‑149 (MQ=255)
                                                                                                                                   ttgTCTTTGCATGGCCAGGCAAAGCAAGGTGCTGGAGAACCGCAATAAAATGCTGAAGGCTTAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTtgtg                <  2:47136/147‑1 (MQ=255)
                                                                                                                                   tCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGATGAAGGCTCAGGGGATTAACGCGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGACTTAAGTtgtg                >  1:195604/1‑149 (MQ=255)
                                                                                                                                    ttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcaca                                           >  2:120256/4‑121 (MQ=255)
                                                                                                                                    ttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcaca                                           <  1:120256/118‑1 (MQ=255)
                                                                                                                                         ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAAGGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTg          <  1:116819/149‑1 (MQ=255)
                                                                                                                                                cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCGCAGTATTCAGCCTTCTGAAGTAAGTTGTGCTCTTGGatcatc   >  1:318889/1‑149 (MQ=255)
                                                                                                                                                 acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTGTTGCTCTTGGATCATcc  >  2:203474/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCC  >  NC_000913_3_pae_tpiA/2810729‑2811021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.