Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,756,121	Δ557 bp	coding (424‑980/1413 nt)	pykF →	pyruvate kinase I

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1756121	1756677	557	84 [0]	[0] 86	pykF	pyruvate kinase I

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1756120	0 (0.000)	84 (1.010)	68/284	0.2	100%	coding (423/1413 nt)	pykF	pyruvate kinase I
	?	NC_000913_3_pae_tpiA	1756678 =	0 (0.000)					coding (981/1413 nt)	pykF	pyruvate kinase I

TTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGC  >  NC_000913_3_pae_tpiA/1755973‑1756266                                                                                                                                                    |                                                                                                                                                   tttCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGCCAACACCGTACTGGTTGACGCTGGTCTGATCGGTATGGAAGTTACAGCCACTCAAGGTAACAAAc                                                                                                                                                   >  2:1427440‑M1/1‑148 (MQ=255)  ttCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAca                                                                                                                                                  <  2:1208583‑M1/149‑3 (MQ=255)   tCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGTTATGGAAGTTACCGCCATTGAAGGTAACAAAcat                                                                                                                                                 <  2:933375‑M1/149‑4 (MQ=255)      ccacgcataaatcTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGGCTGTGGGCAACACCGTACTTGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggc                                                                                                                                              <  1:56317‑M1/143‑7 (MQ=255)      ccacTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCGGATCGGTCTGGAAGTTACCGCCATTGAAGGTAACAAAcatggc                                                                                                                                              >  1:425639‑M1/1‑143 (MQ=255)        acTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcga                                                                                                                                            <  2:1402820‑M1/149‑9 (MQ=255)        acTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcga                                                                                                                                            <  1:215998‑M1/149‑9 (MQ=255)        acTAATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcga                                                                                                                                            <  2:33534‑M1/149‑9 (MQ=255)           gATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAACcatggcgacca                                                                                                                                         >  1:147350‑M1/1‑138 (MQ=255)           gATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgacca                                                                                                                                         >  2:578016‑M1/1‑138 (MQ=255)            aTAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccat                                                                                                                                        >  1:1390379‑M1/1‑137 (MQ=255)               aaTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctg                                                                                                                                     <  1:605587‑M1/149‑16 (MQ=255)               aaTCCGTTAACGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctg                                                                                                                                     <  1:1032793‑M1/149‑16 (MQ=255)                 tCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcg                                                                                                                                   >  1:965389‑M1/1‑132 (MQ=255)                 tCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcg                                                                                                                                   >  1:493950‑M1/1‑132 (MQ=255)                   tGTTATCGGCAACAGCGAAAGGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaa                                                                                                                                 <  1:659678‑M1/149‑20 (MQ=255)                    gTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGACATTGAAGGTAACAAAcatggcgaccatctgcgaac                                                                                                                                <  2:90495‑M1/149‑21 (MQ=255)                        tCGGCATCAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtac                                                                                                                            <  1:809069‑M1/149‑25 (MQ=255)                        tCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgcccatctgcgaacgtac                                                                                                                            >  1:236784‑M1/1‑125 (MQ=255)                        tCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtac                                                                                                                            >  1:1397678‑M1/1‑125 (MQ=255)                         cGGGAAAAGCGGAATGGTTGGGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTGGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtacc                                                                                                                           <  2:147350‑M1/149‑26 (MQ=255)                           gCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccga                                                                                                                         >  1:1358596‑M1/1‑122 (MQ=255)                                 gCGAAATGGTTGCGGTAACGTATGAAGGTTTCAATACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgt                                                                                                                   <  1:891449‑M1/149‑34 (MQ=255)                                   gAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtga                                                                                                                 <  1:936049‑M1/149‑36 (MQ=255)                                   gAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtga                                                                                                                 >  1:502650‑M1/1‑114 (MQ=255)                                      aTGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatga                                                                                                              <  1:845581‑M1/149‑39 (MQ=255)                                       gggtGGCGGTTACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgacgatctgcgaacgtaccgaccgcgtgatgaa                                                                                                             <  2:218632‑M1/148‑40 (MQ=255)                                                tAACGTATGAAGGTTTCACTAGTGACCTGTCTGTTGGCACCACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtct                                                                                                    <  2:1289211‑M1/149‑49 (MQ=255)                                                 aaCGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctc                                                                                                   <  2:502650‑M1/149‑50 (MQ=255)                                                     tATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagt                                                                                               >  2:40123‑M1/1‑96 (MQ=255)                                                       tGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCCAACTTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttc                                                                                             <  1:927497‑M1/149‑56 (MQ=255)                                                        gAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttca                                                                                            >  1:745067‑M1/1‑93 (MQ=255)                                                        gAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttca                                                                                            >  1:1164259‑M1/1‑93 (MQ=255)                                                        gAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttca                                                                                            >  1:1164319‑M1/1‑93 (MQ=255)                                                           gttttcaCTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaaca                                                                                         <  2:499936‑M1/147‑60 (MQ=255)                                                              ttCACTACTGACCTGTCTGTTGGGAAAACCGTTCTGGTTGGCGATGGTATGATTGTTATTGAAGTTACCGCCAGTGAGGGGAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatg                                                                                      <  2:491274‑M1/149‑63 (MQ=255)                                                               tCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatgtcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttgaacaattc                                                                                     >  2:369705‑M1/1‑86 (MQ=255)                                                                 acgactgacCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgaca                                                                                   <  2:231921‑M1/146‑66 (MQ=255)                                                                   tactGACCTGTCTGTTGGCAACACCGTACTGGTTGACGCTGGTCTGATCGGTATGGAAGTTACCGCCATCGAAGGTAACAAAcatggcgaccatctgcgaaggtaccgaccgcgttatgaacagccgtctcgagctcaacaatgacacc                                                                                 >  2:626439‑M1/1‑82 (MQ=255)                                                                   tactGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaac                                                                                 >  1:108461‑M1/1‑82 (MQ=255)                                                                          ctgtctgtTGGCAACACCGTACTGGTTGAGGATGGTCTAATCGGTATGGACGTTACCGCCATTGAAGGTAACAAAcatggcgaccagctgcgaacgtaccgactgcgtgatgaacagccgtctcgagttcaacaatgacaaccgcaaac                                                                          >  1:1225159‑M1/1‑75 (MQ=255)                                                                          ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatg                                                                                                                                                <  1:1207774‑M1/79‑5 (MQ=255)                                                                          ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatg                                                                                                                                                >  2:1207774‑M1/1‑75 (MQ=255)                                                                               tgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgacagcgtgatgaacagccgtctggggttcaacgatgacaaccgtccactgccc                                                                     >  1:1415650‑M1/1‑70 (MQ=255)                                                                                 ttGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcat                                                                   >  1:513261‑M1/1‑68 (MQ=255)                                                                                    gCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgtgttttacaatgacaaacgtatagtgcgcgttac                                                                >  2:412619‑M1/1‑65 (MQ=255)                                                                                      aaCACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgta                                                                                                                             >  1:1401405‑M1/1‑63 (MQ=255)                                                                                      aaCACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgta                                                                                                                             <  2:1401405‑M1/86‑24 (MQ=255)                                                                                         acCGTACTGGTTGACGATGGTCTGATCGGTATGTAAATTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgacagcgtgatgaacagccgtctcgagttcaacaatgacaaccgataatcacgcattaccgaaa                                                           >  1:350427‑M1/1‑60 (MQ=255)                                                                                            gTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaacctgcgcattaccgaagcgg                                                        <  1:578016‑M1/149‑93 (MQ=255)                                                                                                 ggTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgc                                                   >  1:1172813‑M1/1‑52 (MQ=255)                                                                                                  gTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtctgcc                                                  >  2:743167‑M1/1‑51 (MQ=255)                                                                                                      aCGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaacctgcgcattaccgtagcggtatcccgtgg                                              >  2:227316‑M1/1‑47 (MQ=255)                                                                                                      aCGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgcgg                                              >  1:907169‑M1/1‑47 (MQ=255)                                                                                                        gATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaactgcggtctcgaggtcaacaatgacaaccgtaaactgcgcattaccgacgcggtatgccgtggtg                                            >  1:701027‑M1/1‑45 (MQ=255)                                                                                                        gATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtg                                            >  1:1349878‑M1/1‑45 (MQ=255)                                                                                                          ttgtcTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatcccaaaggttgagtgcgcattaccgaagcggtatgccgtggtgcc                                          <  1:1431743‑M1/147‑107 (MQ=255)                                                                                                           ggTCTGATCGGTATGGAAGTTACCGCTATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagcggtctcgagttcaacgatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccg                                         <  1:1274358‑M1/149‑108 (MQ=255)                                                                                                            ggctgATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgt                                        <  1:968659‑M1/147‑109 (MQ=255)                                                                                                             tCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaacagcccattacagaagcggtatcccgaggtgccgtt                                       >  2:332503‑M1/1‑40 (MQ=255)                                                                                                              cTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttc                                                                                             >  2:982778‑M1/1‑39 (MQ=255)                                                                                                              cTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttc                                                                                             <  1:982778‑M1/94‑56 (MQ=255)                                                                                                               tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttga                                     >  1:69292‑M1/1‑38 (MQ=255)                                                                                                                   cGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgccttaccgaagcggtatgccgtggtgccgttgaaact                                 >  1:119061‑M1/1‑34 (MQ=255)                                                                                                                     gTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcgttaccgaagcggtataccgtggtgccgttgaaactgc                               >  2:782496‑M1/1‑32 (MQ=255)                                                                                                                     gTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggagaccctgttcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgc                               <  1:269344‑M1/149‑118 (MQ=255)                                                                                                                      tacggaagTTACCGCTATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgct                              <  2:216797‑M1/146‑119 (MQ=255)                                                                                                                        tGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctga                            >  2:559772‑M1/1‑29 (MQ=255)                                                                                                                         ggAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatga                                                                                                              >  2:1305228‑M1/1‑28 (MQ=255)                                                                                                                         ggAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatga                                                                                                              <  1:1305228‑M1/66‑39 (MQ=255)                                                                                                                                  cGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgaggtcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaagactggat                  >  1:542598‑M1/1‑19 (MQ=255)                                                                                                                                    ccATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgcaaaacttgatgc                >  1:168349‑M1/1‑17 (MQ=255)                                                                                                                                    ccATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggt                                             <  1:956119‑M1/120‑104 (MQ=255)                                                                                                                                    ccATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggt                                             >  2:956119‑M1/1‑17 (MQ=255)                                                                                                                                      aTTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgacaacctggatgctc              >  2:653941‑M1/1‑15 (MQ=255)                                                                                                                                          aaGGTAACAAAcatggcgaccatctgcgaacgtaccgactgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgtcgacactgcttaaaaactggatgctccgct          >  1:1345883‑M1/1‑11 (MQ=255)                                                                                                                                           aGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctg         >  1:967612‑M1/1‑10 (MQ=255)                                                                                                                                             gTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgat       >  1:369013‑M1/1‑8 (MQ=255)                                                                                                                                              tAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcatttccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatc      <  1:1361197‑M1/149‑143 (MQ=255)                                                                                                                                               aacaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcg     >  2:1160729‑M1/1‑6 (MQ=255)                                                                                                                                                acaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgt    <  1:595135‑M1/149‑145 (MQ=255)                                                                                                                                                acaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactacgcattaacgaagcggtatgccggggttccgttgaacctgctgaagaaatagatgctcctctggtcgt    >  2:271543‑M1/1‑5 (MQ=255)                                                                                                                                                  aaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgtgg  >  1:1183713‑M1/1‑3 (MQ=255)                                                                                                                                                  aaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgtgg  >  1:702921‑M1/1‑3 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGC  >  NC_000913_3_pae_tpiA/1755973‑1756266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑