New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 349969NA (NA)6 (0.190)
+CTACAGTTCAGTTCACCCCGTAGGCCTGATAAGTCGCGTCAGCGGCGCATCAGGCATCG
4/166 4.8 NA noncoding (365/365 nt) REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences
?NC_000913_3_pae_tpiA = 349972 NA (NA)intergenic (+400/‑40) prpB/prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
Rejected: Coverage evenness skew score above cutoff.

CTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGT  >  NC_000913_3_pae_tpiA/349823‑350115
                                                                                                                                                   |                                                                                                                                                  
cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGacaa                                                                                                                                                  >  1:929236/1‑149 (MQ=32)
cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGacaa                                                                                                                                                  >  1:723614/1‑149 (MQ=32)
       ttGCACGGAACTGTAGGTCGGACAAGACGCATAGCGTCGCATCCGACAACCGATGACTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc                                                                                                                                           <  1:8494/148‑1 (MQ=255)
       gTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc                                                                                                                                           <  2:723614/149‑1 (MQ=38)
         gCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTTGCATCCGACAACAATCTCGa                                                                                                                                         >  2:25282/1‑149 (MQ=255)
          cattggaCTGTAGGGGCGATAAGACGGATGGCCACGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc                                                                                                                                        <  2:32174/143‑1 (MQ=11)
          cACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc                                                                                                                                        >  1:531970/1‑149 (MQ=39)
           aCTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATGCGACGCTATGCGTCTTATCCGaccta                                                                                                                                                 >  2:445926/1‑136 (MQ=11)
            cTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAccc                                                                                                                                      <  2:929236/149‑1 (MQ=255)
                  cgTAGGCCGGATGAGACGCATAGCGTCGCATCCGACAACCGATGCCGGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACaaa                                                                                                                                <  1:247412/148‑1 (MQ=255)
                     agttCGGACAAGACGCCTAGCGTCCCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGa                                                                                                                             <  1:287392/146‑1 (MQ=255)
                        tCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATaa                                                                                                                          <  1:880833/149‑1 (MQ=255)
                            aTAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACCGAACTGTAGGTCGGATAAGACGCATAGCGTTGCATCCGACAACAATCTCGACCCTACAAATGATAACAAt                                                                                                                      <  1:25282/149‑1 (MQ=255)
                              aaGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                                                                                                                    <  1:632729/149‑1 (MQ=255)
                                 aCGCATAGCGTCGCATCCGACAACCGATGCCTGATGTGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGAACGCTACTGGCATGTCCGCCAACAATCCCTACCCTACAAATGGTAACAATGACGa                                                                                                                 >  1:685219/1‑149 (MQ=14)
                                 aCGCAACGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGa                                                                                                                 <  2:881291/149‑1 (MQ=255)
                                   gctatgCGTCTTATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAgg                                                                                                               >  2:678738/6‑149 (MQ=255)
                                       ggCGTCGCATCCGACAACCGATGCCTGATGCGCCACTGACGCGACTTATCAGGCCTACGGGGTCAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGacaa                                                                                                           <  1:833519/148‑1 (MQ=255)
                                       aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATg                                                                                                                              >  2:526875/1‑130 (MQ=255)
                                       aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATg                                                                                                                              <  1:526875/130‑1 (MQ=255)
                                           tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                                                                                                                    <  2:753735/136‑1 (MQ=255)
                                           tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                                                                                                                    >  1:753735/1‑136 (MQ=255)
                                              cATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGc                                                                                                    <  1:752638/149‑1 (MQ=255)
                                              cATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGc                                                                                                    >  2:746611/1‑149 (MQ=255)
                                                tCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGa                                                                                                  <  2:602727/149‑1 (MQ=255)
                                                 ccgcgaaCCGATGCCTGATGCGCCGCTGACCCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGCTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGac                                                                                                 <  2:808914/144‑1 (MQ=255)
                                                  cGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGaca                                                                                                <  1:933519/149‑1 (MQ=255)
                                                         cGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGAt                                                                                         <  1:678738/149‑1 (MQ=255)
                                                                tGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGGTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACaa                                                                                                                       >  1:504580/1‑112 (MQ=255)
                                                                tGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACaa                                                                                                                       <  2:504580/112‑1 (MQ=255)
                                                                      gccgcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACAAAACGATCCTGCAAAACAGt                                                                            >  2:568935/1‑149 (MQ=255)
                                                                       gcgcTGACGCGACTTATCAGGCGTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTa                                                                           <  2:126027/148‑1 (MQ=255)
                                                                         gcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTAcc                                                                         <  1:931129/149‑1 (MQ=255)
                                                                                cgACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATcc                                                                                       <  2:903548/128‑1 (MQ=255)
                                                                                cgACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATcc                                                                                       >  1:903548/1‑128 (MQ=255)
                                                                                  aCTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACGTGGCCGACACAACGATCCTGCAAAACAGTACCCATGTCAtt                                                                <  2:630486/149‑1 (MQ=255)
                                                                                    ttATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTaa                                                              >  2:492661/1‑149 (MQ=255)
                                                                                      aTCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAAc                                                            <  2:278916/149‑1 (MQ=255)
                                                                                             cTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGGGGGCCACACCGATCCTGCAGAAGATTCCCCATGCCATTAAAACCaaaca                                                     >  2:204961/1‑147 (MQ=25)
                                                                                                   gctgaactgaacTGTAAGCCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCtgt                                               <  1:447442/147‑1 (MQ=255)
                                                                                                     tgaactgaacTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTgg                                             <  2:22688/149‑1 (MQ=255)
                                                                                                              gcgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    <  1:448352/147‑1 (MQ=255)
                                                                                                              ctgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    <  2:491275/149‑1 (MQ=255)
                                                                                                              ctgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    >  1:491275/1‑149 (MQ=255)
                                                                                                              ctgGAGGGCGGATAAGACGCAGAGCGACGCACCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    <  2:919231/149‑1 (MQ=255)
                                                                                                                gTAGGTCGGATAAGACGCATCGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGc                                  <  1:303489/149‑1 (MQ=255)
                                                                                                                gTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGc                                  <  2:295763/149‑1 (MQ=255)
                                                                                                                  aGGTCGGATAAGACGCATAGCGTCGCAGCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAACAATCTGTGGCACTTTCTGGCGt                                <  2:932142/149‑1 (MQ=255)
                                                                                                                         aTAACACGCATAGCGTCGCATCCGACAACAATCTCGACCCCACAAATGAGAAAAATGACGCGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATCGCATTAAACCGAAAGAATCTGTGACACTTTCTGTCGCTcctacg                         >  2:376583/1‑145 (MQ=255)
                                                                                                                               aCGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCCCTTTCTGGCGTTCCGGCGGGCaa                    >  2:49542/1‑149 (MQ=255)
                                                                                                                               aCGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCaa                    >  1:383137/1‑149 (MQ=255)
                                                                                                                                 gCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATa                  <  1:65792/149‑1 (MQ=255)
                                                                                                                                   tgaGCGTCGGAGCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACg                <  2:134390/147‑1 (MQ=255)
                                                                                                                                      gCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACAATCCTGCAAAACAGAACCCATGTCATTAAACCAAAAAAATCTGTGGCACTTTCTGGCGTTCCCGCGGGCAATACGgcg             >  2:200978/1‑149 (MQ=255)
                                                                                                                                       cGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGgcgc            <  2:531970/149‑1 (MQ=255)
                                                                                                                                                 gACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCAACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGt  >  2:329326/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGT  >  NC_000913_3_pae_tpiA/349823‑350115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.