New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 349969NA (NA)6 (0.190)
+CTACAGTTCAGTTCACCCCGTAGGCCTGATAAGTCGCGTCAGCGGCGCATCAGGCATCG
4/166 4.8 NA noncoding (365/365 nt) REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences REP25 (repetitive extragenic palindromic) element; contains 8 REP sequences
?NC_000913_3_pae_tpiA = 349972 NA (NA)intergenic (+400/‑40) prpB/prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
Rejected: Coverage evenness skew score above cutoff.

GTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGT  >  NC_000913_3_pae_tpiA/349830‑350115
                                                                                                                                               |                                                                                                                                               
ttGCACGGAACTGTAGGTCGGACAAGACGCATAGCGTCGCATCCGACAACCGATGACTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc                                                                                                                                           <  1:8494/148‑1 (MQ=255)
gTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc                                                                                                                                           <  2:723614/149‑1 (MQ=38)
  gCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTTGCATCCGACAACAATCTCGa                                                                                                                                         >  2:25282/1‑149 (MQ=255)
   cattggaCTGTAGGGGCGATAAGACGGATGGCCACGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc                                                                                                                                        <  2:32174/143‑1 (MQ=11)
   cACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc                                                                                                                                        >  1:531970/1‑149 (MQ=39)
     cTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAccc                                                                                                                                      <  2:929236/149‑1 (MQ=255)
           cgTAGGCCGGATGAGACGCATAGCGTCGCATCCGACAACCGATGCCGGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACaaa                                                                                                                                <  1:247412/148‑1 (MQ=255)
              agttCGGACAAGACGCCTAGCGTCCCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGa                                                                                                                             <  1:287392/146‑1 (MQ=255)
                 tCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATaa                                                                                                                          <  1:880833/149‑1 (MQ=255)
                     aTAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACCGAACTGTAGGTCGGATAAGACGCATAGCGTTGCATCCGACAACAATCTCGACCCTACAAATGATAACAAt                                                                                                                      <  1:25282/149‑1 (MQ=255)
                       aaGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                                                                                                                    <  1:632729/149‑1 (MQ=255)
                          aCGCATAGCGTCGCATCCGACAACCGATGCCTGATGTGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGAACGCTACTGGCATGTCCGCCAACAATCCCTACCCTACAAATGGTAACAATGACGa                                                                                                                 >  1:685219/1‑149 (MQ=14)
                          aCGCAACGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGa                                                                                                                 <  2:881291/149‑1 (MQ=255)
                            gctatgCGTCTTATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAgg                                                                                                               >  2:678738/6‑149 (MQ=255)
                                ggCGTCGCATCCGACAACCGATGCCTGATGCGCCACTGACGCGACTTATCAGGCCTACGGGGTCAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGacaa                                                                                                           <  1:833519/148‑1 (MQ=255)
                                aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATg                                                                                                                              <  1:526875/130‑1 (MQ=255)
                                aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATg                                                                                                                              >  2:526875/1‑130 (MQ=255)
                                    tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                                                                                                                    <  2:753735/136‑1 (MQ=255)
                                    tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGa                                                                                                                    >  1:753735/1‑136 (MQ=255)
                                       cATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGc                                                                                                    <  1:752638/149‑1 (MQ=255)
                                       cATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGc                                                                                                    >  2:746611/1‑149 (MQ=255)
                                         tCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGa                                                                                                  <  2:602727/149‑1 (MQ=255)
                                          ccgcgaaCCGATGCCTGATGCGCCGCTGACCCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGCTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGac                                                                                                 <  2:808914/144‑1 (MQ=255)
                                           cGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGaca                                                                                                <  1:933519/149‑1 (MQ=255)
                                                  cGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGAt                                                                                         <  1:678738/149‑1 (MQ=255)
                                                         tGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGGTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACaa                                                                                                                       >  1:504580/1‑112 (MQ=255)
                                                         tGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACaa                                                                                                                       <  2:504580/112‑1 (MQ=255)
                                                               gccgcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACAAAACGATCCTGCAAAACAGt                                                                            >  2:568935/1‑149 (MQ=255)
                                                                gcgcTGACGCGACTTATCAGGCGTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTa                                                                           <  2:126027/148‑1 (MQ=255)
                                                                  gcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTAcc                                                                         <  1:931129/149‑1 (MQ=255)
                                                                         cgACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATcc                                                                                       <  2:903548/128‑1 (MQ=255)
                                                                         cgACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATcc                                                                                       >  1:903548/1‑128 (MQ=255)
                                                                           aCTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACGTGGCCGACACAACGATCCTGCAAAACAGTACCCATGTCAtt                                                                <  2:630486/149‑1 (MQ=255)
                                                                             ttATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTaa                                                              >  2:492661/1‑149 (MQ=255)
                                                                               aTCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAAc                                                            <  2:278916/149‑1 (MQ=255)
                                                                                      cTACGGGGTGAACTGAACTGTAGGTCGGCTAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGGGGGCCACACCGATCCTGCAGAAGATTCCCCATGCCATTAAAACCaaaca                                                     >  2:204961/1‑147 (MQ=25)
                                                                                            gctgaactgaacTGTAAGCCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCtgt                                               <  1:447442/147‑1 (MQ=255)
                                                                                              tgaactgaacTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTgg                                             <  2:22688/149‑1 (MQ=255)
                                                                                                       gcgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    <  1:448352/147‑1 (MQ=255)
                                                                                                       ctgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    <  2:491275/149‑1 (MQ=255)
                                                                                                       ctgTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    >  1:491275/1‑149 (MQ=255)
                                                                                                       ctgGAGGGCGGATAAGACGCAGAGCGACGCACCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTg                                    <  2:919231/149‑1 (MQ=255)
                                                                                                         gTAGGTCGGATAAGACGCATCGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGc                                  <  1:303489/149‑1 (MQ=255)
                                                                                                         gTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGc                                  <  2:295763/149‑1 (MQ=255)
                                                                                                           aGGTCGGATAAGACGCATAGCGTCGCAGCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAACAATCTGTGGCACTTTCTGGCGt                                <  2:932142/149‑1 (MQ=255)
                                                                                                                  aTAACACGCATAGCGTCGCATCCGACAACAATCTCGACCCCACAAATGAGAAAAATGACGCGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATCGCATTAAACCGAAAGAATCTGTGACACTTTCTGTCGCTcctacg                         >  2:376583/1‑145 (MQ=255)
                                                                                                                        aCGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCCCTTTCTGGCGTTCCGGCGGGCaa                    >  2:49542/1‑149 (MQ=255)
                                                                                                                        aCGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCaa                    >  1:383137/1‑149 (MQ=255)
                                                                                                                          gCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATa                  <  1:65792/149‑1 (MQ=255)
                                                                                                                            tgaGCGTCGGAGCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACg                <  2:134390/147‑1 (MQ=255)
                                                                                                                               gCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACAATCCTGCAAAACAGAACCCATGTCATTAAACCAAAAAAATCTGTGGCACTTTCTGGCGTTCCCGCGGGCAATACGgcg             >  2:200978/1‑149 (MQ=255)
                                                                                                                                cGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGgcgc            <  2:531970/149‑1 (MQ=255)
                                                                                                                                          gACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCAACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGt  >  2:329326/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGT  >  NC_000913_3_pae_tpiA/349830‑350115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.