New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3037834	49 (0.920)	8 (0.160)	8/268	5.4	14.3%	coding (273/1734 nt)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
	?	NC_000913_3_pae_tpiA	= 3037842	50 (1.000)					coding (265/1734 nt)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGT  >  NC_000913_3_pae_tpiA/3037700‑3037987                                                                                                                                               |                                                                                                                                                  aCCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTACCGGGCTTAAGCCGCAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCGGCACGCGTAGAGCCGTGCt                                                                                                                                             >  1:823532/1‑149 (MQ=255)    gTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCCTAAGCCTTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCAATATTACTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTggc                                                                                                                                          >  1:31643/1‑148 (MQ=255)     tGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTc                                                                                                                                         >  1:107196/1‑149 (MQ=255)                           ggCATGGGCCTGACCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATGGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCAc                                                                                                                   <  2:181048/149‑1 (MQ=255)                            gCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCAcc                                                                                                                  >  1:47946/1‑149 (MQ=255)                              aTGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGa                                                                                                                >  1:339983/1‑149 (MQ=255)                               tGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGGCTTCGAAACGGTTTGGTGCCAGGAAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACACTTAGATCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGAc                                                                                                               >  2:601992/1‑149 (MQ=255)                                gggCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGAcc                                                                                                              >  1:106084/1‑149 (MQ=255)                                   ccTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAATTCACCGACcaca                                                                                                           >  1:434924/1‑149 (MQ=255)                                   ccTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACcaca                                                                                                           >  1:480393/1‑149 (MQ=255)                                   ccTGATCGACCACCTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACcaca                                                                                                           >  2:434587/1‑149 (MQ=255)                                         cGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATc                                                                                                     <  1:297286/149‑1 (MQ=255)                                           aCCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCcg                                                                                                   >  1:791945/1‑149 (MQ=255)                                                ctgcGGGCGTAAGCCGTAAGCGCTGCCGAAGCGGTTTGGTACCAGGGAGTGGATATTGCTGCAACCAAGCGTGCGCACCGCCAGCACGTTTAGAGCCCTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTc                                                                                              <  2:179982/146‑1 (MQ=255)                                                   cGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCtt                                                                                           <  1:253478/149‑1 (MQ=255)                                                    gggCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGAATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTc                                                                                          >  1:417763/1‑149 (MQ=255)                                                     ggCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCGACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTcg                                                                                         <  1:888421/149‑1 (MQ=255)                                                      gCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTcgc                                                                                        >  2:136944/1‑149 (MQ=255)                                                                    cGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTaa                                                                          >  1:235181/1‑149 (MQ=255)                                                                    cGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTaa                                                                          >  1:126813/1‑149 (MQ=255)                                                                      tCTTCGAAACGGTTTGGTACCAAGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGGGCCGTGCTGGTCGCGCCGTCGGCGCCGACACCCCGTACGACAATAACCCGACTTCCTTCCCCTACCACGTTGTAAAg                                                                        >  1:730205/1‑149 (MQ=255)                                                                        ttCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAGGCGTTGTGAAGGa                                                                      >  2:351179/1‑149 (MQ=255)                                                                        ttCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGa                                                                      >  2:521625/1‑149 (MQ=255)                                                                         tCGAAACTGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATAGTCCGCGCGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGAt                                                                     <  1:688218/149‑1 (MQ=255)                                                                         gggagaCGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGAt                                                                     <  2:703881/144‑1 (MQ=255)                                                                          cGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGAtc                                                                    <  1:521625/149‑1 (MQ=255)                                                                            aaaCGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGAtctc                                                                  >  2:593992/1‑149 (MQ=255)                                                                            aaaCGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGAtatc                                                                  >  1:526629/1‑149 (MQ=255)                                                                               cGGTTTGGTACCAGGTAGTCGATATTGCGGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAAc                                                               <  1:778026/149‑1 (MQ=255)                                                                               cGGTGTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGGCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAAc                                                               <  2:640055/149‑1 (MQ=255)                                                                                ggTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACg                                                              <  2:47946/149‑1 (MQ=255)                                                                                  tttGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGc                                                            >  2:598622/1‑149 (MQ=255)                                                                                       tACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGGCCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCtttt                                                       >  1:886539/1‑149 (MQ=255)                                                                                       tACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGACAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCtttt                                                       <  1:645749/149‑1 (MQ=255)                                                                                           aGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATc                                                                                                     >  1:599586/1‑99 (MQ=255)                                                                                           aGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATc                                                                                                     <  2:599586/99‑1 (MQ=255)                                                                                            cgTAGTCGATATGGGTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGAcg                                                  <  2:722983/148‑1 (MQ=255)                                                                                             tgagccGACATTGCCGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGAcgc                                                 <  2:498921/144‑1 (MQ=255)                                                                                                   gATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCa                                           <  1:593992/149‑1 (MQ=255)                                                                                                   gATATGGCGGCAACCAAGCGGGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGTCCACAATACTCCGCGTCCCGTGGCTAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCa                                           <  1:884389/149‑1 (MQ=255)                                                                                                     tatTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGt                                         <  2:126813/149‑1 (MQ=255)                                                                                                             cAACCAAGCGAGCGCATGGCCAGCACGCTCAGAGCCGTGCTGGTCGCGCCGTCGTCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCa                                 <  1:806364/149‑1 (MQ=255)                                                                                                                  aaGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTtg                                       >  2:536132/1‑138 (MQ=255)                                                                                                                  aaGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTtg                                       <  1:536132/138‑1 (MQ=255)                                                                                                                   agcgagcgCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGGCGTCGGCGGCGAAATCACCGACCACAATAATCCGCGATCCTCCGCGAAAGGCGTGGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGgcag                           <  2:809782/149‑1 (MQ=255)                                                                                                                   agcgagcgCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCATACGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGgcag                           >  1:456828/1‑149 (MQ=255)                                                                                                                     cgagcgCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCGGTTGCTGCCAGGgcagca                         >  1:602877/1‑149 (MQ=255)                                                                                                                              cGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTaa                <  1:434587/149‑1 (MQ=255)                                                                                                                                 cAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCGGCGTTCCTTCCCGAAAATCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTACCAGGGCACCCTACCTTAAacac             >  1:367265/1‑149 (MQ=255)                                                                                                                                   gCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTg           <  2:791945/149‑1 (MQ=255)                                                                                                                                         ttAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCGGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACCCTGCGTTcc     >  1:757173/1‑149 (MQ=255)                                                                                                                                            gagCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGt  >  2:650371/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                  ACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGT  >  NC_000913_3_pae_tpiA/3037700‑3037987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.