New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 157995 =251 (0.880)9 (0.030) 6/430 23.1 3.6% coding (478/648 nt) TMARI_RS00740 lactate utilization protein
?NC_021214 158204 = 246 (0.900)coding (1857/1872 nt) TMARI_RS00745 DUF4914 domain‑containing protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGACGACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_021214/158241‑157995
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aattcatcggTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGAAAAACCGA  >  NC_021214/158204‑158442
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
TCGACGTCGTATCTCTCGATGACTATCTCAATATCATACCGATGAATTTTTAAAGCGGGTAAAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAAT                                                                                                                                                                                                                                                        <  1:227469/240‑1
TCGACGACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAGGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAAT                                                                                                                                                                                                                                                        <  2:276716/240‑1
  GACGACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTC                                                                                                                                                                                                                                                      >  1:83825/1‑240
     GACGGATCTCTCGATGACTATCTCAAGATCATACCGATGTATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATC                                                                                                                                                                                                                                                   >  1:1171194/1‑240
     GACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATC                                                                                                                                                                                                                                                   >  2:266111/1‑240
     GACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATC                                                                                                                                                                                                                                                   >  2:540875/1‑240
     GACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTATGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATC                                                                                                                                                                                                                                                   >  2:188341/1‑240
       CGGATCTCTCGATGACTATTTCAAGATCAACGCGATGAATGTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGGAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGG                                                                                                                                                                                                                                                 <  1:523927/240‑1
                                        GATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAAT                                                                                                                                                                                                                                                        >  1:1099984/1‑200
                                        GATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAAT                                                                                                                                                                                                                                                        <  2:1099984/200‑1
                                                                   CCCGCTTTTTTAAAGTCCGACGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCT                                                                                                                                                                                     >  2:1142818/1‑240
                                                                   CCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCT                                                                                                                                                                                     >  1:755492/1‑240
                                                                   CCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCT                                                                                                                                                                                     >  2:607708/1‑240
                                                                   CCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCT                                                                                                                                                                                     >  1:255343/1‑240
                                                                     CGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTT                                                                                                                                                                                    >  1:94695/1‑239
                                                                                                                GAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCT                                                                                                                                        <  1:607708/240‑1
                                                                                                                                                CTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTT                                                                                                        <  2:255343/240‑1
                                                                                                                                                                                                           TTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGA                                             <  2:755492/240‑1
                                                                                                                                                                                                             TGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAAT                                           <  2:94695/240‑1
                                                                                                                                                                                                                                              ATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCTCCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGA          <  1:1149118/240‑1
                                                                                                                                                                                                                                              ATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGA          >  1:78538/1‑240
                                                                                                                                                                                                                                              ATTCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGA          <  1:59067/240‑1
                                                                                                                                                                                                                                                TCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGCTCTTCATTTCTTTGAGAGAAA        >  1:6150/1‑240
                                                                                                                                                                                                                                                TCATCGGTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGAAA        >  2:988029/1‑240
                                                                                                                                                                                                                                                      GTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCaatcc                                                          >  2:364331/1‑179
                                                                                                                                                                                                                                                      GTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCaatcc                                                          <  1:364331/184‑6
                                                                                                                                                                                                                                                      GTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGAAAAACCGA  >  1:954414/1‑240
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
TCGACGACGGATCTCTCGATGACTATCTCAAGATCATACCGATGAATTTTTAAAGCGGGGAGAATTCCCCGCTTTTTTAAAGTCCGAAGTCTTCCAGCGTCAGAATCACCTTGAACCTTCCCGGCTGTCTCACCCCAGATTCCACTACCAGAAAGTAGTCACAGATCCTCTGAGGAGAAGAACAGTCTGCACAGAATCCTGTTTTTGTGCACGGTGTTTCCAGATTCAGTCGTTTCGAATTCATCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_021214/158241‑157995
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aattcatcggTATGATCTTGAGATAGTCATCGAGAGATCCGTCGTCGAGACAGCACTGGATGATTTCTCTTCCCACGGGATTCAGGTCTTCGGTCAGGAATTTTTTCAGTTCTCTCTTGAAGAAGTCTTTCAACTCTTGGGCACCTTTTTCATAAGCTTCCTCACCTACTTCGGATTGCAAGTGTGGCTGCAGAAGTTCTTTCGGAATGGTGTTTCCTTCGATCTTCATCTCTTTGAGAGAAAAACCGA  >  NC_021214/158204‑158442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.