Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 B.subtilis KO7-s Illumina/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A9 F56 I0 R1 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A9 F56 I13 R1 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A10 F54 I0 R2 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A10 F54 I2 R2 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A11 F58 I0 R3 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A11 F58 I1 R3 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A12 F59 I0 R4 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A12 F59 I7 R4 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']
A9101112 F55 I0 R1 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415168 B.subtilis KO7-s Illumina/ ['/var/data/pSIJ663_pHT315.gb', '/var/data/CP006881_PY79ref.gb']