Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415171 B.subtilis KO7-s Illumina/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A17 F55 I0 R1 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A17 F55 I4 R1 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A18 F53 I0 R2 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A18 F53 I5 R2 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A19 F53 I0 R3 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A19 F53 I19 R3 clonal The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A20 F53 I0 R4 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A20 F53 I6 R4 clonal Supplemented Hydrolysate, TALEd with synthetic M9H.The trace element solution is specific to B.subtilis, provided by Sheila Jensen. Hydrolysate-M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415167 Engineered B.subtilis KO7-s plexWell/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']
A17181920 F51 I0 R1 population The trace element solution is specific to B.subtilis, provided by Sheila Jensen. M9 Glucose(30),Xylose(10),Arabinose(5) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) erythromycin(10) sup, 37 1415172 B.subtilis KO7-s Illumina/ ['/var/data/psij663pht315_arae_xylab-sgc7sg13-promoter.gb', '/var/data/CP006881_PY79ref.gb']