Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal M9 NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) 37 679895 E. coli BW25113 delta-gpmA,gpmM,pfkA,pfkB,zwf,aceBAK pCBB ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']
A1 F1 I1 R1 clonal xylose-limiting condition; To avoid the possibility that pyruvate becomes the limiting nutrient instead of xylose, in the second and fourth experiments, xylose concentrations were lowered in the feed media whenever a significant drop in residual pyruvate was detected in the culture M9 sodium pyruvate(5 g/L),xylose(25 mg/L),carbon dioxide(25%) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) chloramphenicol(34 mg/L), 37 679895 E. coli BW25113 delta-gpmA,gpmM,pfkA,pfkB,zwf,aceBAK pCBB; ancestral strain inoculated contained a ∆mutS mutation ['/var/data/KX077536.1.gb', '/var/data/CP009273.1.gb']