Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A1 F1 I1 R1 clonal Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. RPMI Ca(NO3)2(4H2O)(0.1) Na2HPO4(0.8) MgSO4(0.04884) None ,MMV668399(high dose intermittent exposure) 37 36329 BMGF-Wirth-3D7-11-1-13 Illumina/ /var/data/GCF_000002765.5.gb
A2 F1 I1 R1 clonal Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. RPMI Ca(NO3)2(4H2O)(0.1) Na2HPO4(0.8) MgSO4(0.04884) None ,MMV668399(high dose intermittent exposure) 37 36329 BMGF-Wirth-3D7-11-1-13 Illumina/ /var/data/GCF_000002765.5.gb
A3 F1 I1 R1 clonal Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. RPMI Ca(NO3)2(4H2O)(0.1) Na2HPO4(0.8) MgSO4(0.04884) None ,MMV668399(high dose intermittent exposure) 37 36329 BMGF-Wirth-3D7-11-1-13 Illumina/ /var/data/GCF_000002765.5.gb
A4 F1 I1 R1 clonal Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. RPMI Ca(NO3)2(4H2O)(0.1) Na2HPO4(0.8) MgSO4(0.04884) None ,MMV668399(high dose intermittent exposure) 37 36329 BMGF-Wirth-3D7-11-1-13 Illumina/ /var/data/GCF_000002765.5.gb
A5 F1 I1 R1 clonal Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. RPMI Ca(NO3)2(4H2O)(0.1) Na2HPO4(0.8) MgSO4(0.04884) None ,MMV668399(high dose intermittent exposure) 37 36329 BMGF-Wirth-3D7-11-1-13 Illumina/ /var/data/GCF_000002765.5.gb