Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A1 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A1 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A2 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A2 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A3 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A3 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A4 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A4 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A5 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A5 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A6 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A6 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A7 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A7 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A8 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A8 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A9 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A9 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A10 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A10 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A11 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A11 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A12 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A12 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A13 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A13 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A14 F1 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb
A14 F1 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1g/L) KH2PO4(3g/L),Na2HPO4(6.78g/L) MgSO4(1mM) CaCl2(0.1mM) Carbenicillin(50), 37 511145 E. coli K-12 MG1655 with all UAG stop codons replaced with UAA. Illumina/ 150 NC_000913_3.gb