Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F16 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F17 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F22 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F23 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F25 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F30 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F41 I0 R1 population The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb
A1 F66 I1 R1 clonal The medium is mixing a ratio of HDA and MOPS-EZ with 400 mM of MeOH, HDA:MOPS = 100% other MeOH(400) None None None None 37 679895 BW25113 ∆rpiA::FRT ∆rpiB ∆nupG::PLlacO1::medh/tkt(Mb)/tal(Kp)/hps(Mb)/phi SS3(intergenic site)::PLlacO1::medh/hps(Bm)/phi, and then ∆pfkA ∆gapA::gapC with pFC139 Illumina/ /var/data/CP009273_1.gb