Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F4 I5 R1 74 1140.6 72036873 99.2% 71460578 99.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
MC JC AE015451_phaM1 5,393,059 Δ5,661 bp [hsdR CDS (Type I restriction enzyme EcoAI R protein)][hsdS CDS (Type I restriction‑modification system_ S subunit)] [hsdR CDS (Type I restriction enzyme EcoAI R protein)], hsdM CDS (Type I restriction enzyme EcoEI M protein), [hsdS CDS (Type I restriction‑modification system_ S subunit)]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AE015451_phaM1 5393059 5398719 5661 1767 [0] [0] 1780 [hsdR CDS (Type I restriction enzyme EcoAI R protein)]–[hsdS CDS (Type I restriction‑modification system_ S subunit)] [hsdR CDS (Type I restriction enzyme EcoAI R protein)],hsdM CDS (Type I restriction enzyme EcoEI M protein),[hsdS CDS (Type I restriction‑modification system_ S subunit)]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 53930580 (0.000)1763 (1.540) 196/196 -0.0 100% intergenic (‑386/‑1) PP_5710 CDS (exported protein of unknown function)/hsdR CDS (Type I restriction enzyme EcoAI R protein) –/–
?AE015451_phaM1 5398720 = 0 (0.000)intergenic (+1/‑10) hsdS CDS (Type I restriction‑modification system_ S subunit)/PP_5711 CDS (protein of unknown function) –/–

GATK/CNVnator alignment

N/A