Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F14 I0 R1
|
206 |
76.7 |
3255580 |
98.8% |
3216513 |
146.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
4,356,305 |
T→G |
26.1% |
V239G (GTC→GGC) |
modC → |
molybdate ABC transporter ATP‑binding protein ModC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 4,356,305 | 0 | T | G | 26.1%
| 81.4
/ 5.8
| 48 | V239G (GTC→GGC) | modC | molybdate ABC transporter ATP‑binding protein ModC |
| Reads supporting (aligned to +/- strand): ref base T (11/24); new base G (11/2); total (22/26) |
| Fisher's exact test for biased strand distribution p-value = 2.43e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCAGCCACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCC > NC_002947_CJ‑RC/4356164‑4356448
|
tCAGCCACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCgtgt < 7:362041/147‑1 (MQ=255)
ccACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGaa < 5:181861/148‑1 (MQ=255)
cACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGGGGTGttggtag > 5:331467/1‑143 (MQ=255)
cACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGGGGTAGAAg > 8:306453/1‑148 (MQ=255)
cGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGGCGTGGTGGAAGGt > 6:367327/1‑148 (MQ=255)
cAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGGCGGGGTGGTagggaggg > 5:348623/1‑148 (MQ=255)
cAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAggg > 6:4005/1‑148 (MQ=255)
cAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCCGCGGCCTGTGGGAACGGAgggg > 3:270644/1‑148 (MQ=255)
gATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGggtgg < 8:382786/148‑1 (MQ=255)
aTGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGggtggt < 6:281451/148‑1 (MQ=255)
gAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGGGGGGGTAGAAGGTAGGGGGGtttt > 4:93795/1‑147 (MQ=255)
aaGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTg < 5:326464/147‑1 (MQ=255)
gcgcgGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCTGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGa < 2:135316/149‑1 (MQ=255)
cgGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGAcc < 8:98562/148‑1 (MQ=255)
gCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCAt < 1:320350/147‑1 (MQ=255)
gCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGGGGTGGAAGGTTGGGTGGTTGGGCACGACCCGCAtt > 1:321924/1‑148 (MQ=255)
cGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTAc < 6:159200/148‑1 (MQ=255)
tGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACCCCCGCGTCGTGGTCGAAGGTAGGGTGGTTGGGCACCACCCCCAATACGGCctg > 6:285046/1‑146 (MQ=255)
tgtTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGAc < 2:276687/149‑1 (MQ=255)
cTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGGCGGGGTGGGAGGTAGGGTGGTTGGGGCCGCCCCCCCGTTAGGCCGGCTGGACCAgc > 5:204109/1‑148 (MQ=255)
gAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCt < 4:329771/149‑1 (MQ=255)
gAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGGCGGGTTCGAACGTTGGGTGGTTGGGGACGACCCGCATTACGGGCAGCTGGACCTGCGcc > 6:82630/1‑148 (MQ=255)
aGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGccc < 5:259315/148‑1 (MQ=255)
ggCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGGCGTGTTGGAAGGTAGGGGGGTTTGGCACGACCCGCAATACGGCCTGCTGGACCTGCGCCTGCCCg > 8:164157/1‑148 (MQ=255)
gggCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGgcagc < 1:139919/149‑1 (MQ=255)
aTCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCgg < 2:326232/149‑1 (MQ=255)
ccAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGCCCCCGGCGGCGGGTTGGTAGGTAGGGGGGGTGGGGACGCCCCCCAATAACGCCGGGGGGGACAGCGCCTGCCCCGCAGCCGCCGGcc > 6:38762/1‑148 (MQ=255)
aGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCgct < 2:371186/149‑1 (MQ=255)
cccGATCGGCCAAACCCTGGCGCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCtgctgc < 1:364209/149‑1 (MQ=255)
cGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACCCCCGCCGCGGGGTGGAAAGTAGGGTGGGTGGGGACCCCCCCCCAAAAGGCCCGCAGGACCAGCGCCGGCCCCGCCCCACCCGGCCCCAGCAGCGCCTCAacc > 4:248209/1‑149 (MQ=255)
gcgcGCCTGGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCa < 1:197574/146‑1 (MQ=255)
cTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGGCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCa < 8:314120/148‑1 (MQ=255)
gCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg < 5:4005/146‑1 (MQ=255)
ccTTGACCTGTCGCTGGCCCAGGGCGACGACCCCCGCGGCGTGTTAGAAGGTAGGGTGGTTGGGCAAGACCCCCATTACCGCCAGCTGGACCAGCGCCAGCCCCGCAGCCGCGGGCCCCAGCTGCGCATCACCCCCCCCGCGCCGGTgg > 2:137460/1‑148 (MQ=255)
cTTGACGTGTCGCGGGCCCAGGGCGAGGACGGCGGGGGCGTGTTCGATGGTAGGGTGTGTGGGCACGACCGGCTTTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGa < 6:231063/148‑1 (MQ=255)
cTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGa < 5:178255/148‑1 (MQ=255)
cTTGACCTGGCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAAGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGAt < 3:34457/149‑1 (MQ=255)
ttGACCTGTCGCTGGCCCAGGGCGACGCCGCCGGCGTCGTGTTGGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATg > 2:326614/1‑149 (MQ=255)
ttGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTGGTGGTGGAAGGTAGGGGGGTTGGGCCCGACCCCCATTAAGGCCTGCTGGACCTGCGCCAGCCCGGCCGCAGCGGGCCGCAGCTGGGCATAAACCACGCCGCGCCGGGGAt > 8:58394/1‑148 (MQ=255)
ccTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGgca < 3:93795/149‑1 (MQ=255)
ccTGTCGCTGGCCCAGGGCGACGACGCCCGCGTCGTGTTAGAAGGTAGGGTGGTTTGGCACGACCCGCAATAAGGCCAGCTGGACCAGCGCCAGCCCCGCAGCCCCGGGCCCCAGCTGCGCAAAACCCACCCCGCCCACGTGATggg > 8:384959/1‑147 (MQ=255)
cTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCAcc > 8:297987/1‑147 (MQ=255)
cAGGGCGACGACGCCGGCGTCGTGTTGGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCCCGCGGCGCAGGTGATTGGCAGCACCCGGcgcg > 8:404601/1‑148 (MQ=255)
gCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCaa < 5:367327/148‑1 (MQ=255)
gacgacGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAgg < 5:285046/148‑1 (MQ=255)
gacgCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCATGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTg > 8:327093/1‑147 (MQ=255)
acgCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCa < 7:306453/148‑1 (MQ=255)
cGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGccc > 4:223506/1‑149 (MQ=255)
|
TCAGCCACGCCCAGGATGAAGTCGCGCGGCTGGCCGACCACCTGGTGTTGCTGGAACAAGGCCGGGCGATCGCCAGCGGCCCGATCGGCGAAACCCTGGCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCC > NC_002947_CJ‑RC/4356164‑4356448
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
