Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F14 I0 R1
|
206 |
76.7 |
3255580 |
98.8% |
3216513 |
146.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
5,512,711 |
A→C |
25.9% |
Q32P (CAG→CCG) |
urtB → |
urea ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 5,512,711 | 0 | A | C | 25.9%
| 59.1
/ 5.6
| 49 | Q32P (CAG→CCG) | urtB | urea ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (19/14); new base C (8/5); total (27/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGCGGGCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCCC > NC_002947_CJ‑RC/5512567‑5512857
|
gAGCGGGCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCggcc > 4:90162/1‑149 (MQ=255)
gAGCGGGCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCggc > 6:239113/1‑148 (MQ=255)
cGGGCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggccgg < 8:407093/148‑1 (MQ=255)
gCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggccggccgc > 3:28250/1‑147 (MQ=255)
cTCACCCGCGAAGAATCCAACTGCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTGACCCTCCTGCTGCTAATCCCGCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTg < 7:244446/148‑1 (MQ=255)
tCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCCAGCCGGCCGGCCGGCt > 1:391180/1‑149 (MQ=255)
aCCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCt < 8:49832/147‑1 (MQ=255)
aCCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCt < 8:56137/147‑1 (MQ=255)
ccGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGa < 5:231796/148‑1 (MQ=255)
cgcgAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGaa < 8:286700/148‑1 (MQ=255)
cgAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCGCGAAgg > 7:246412/1‑148 (MQ=255)
ataaTCCAACACCGATTTCCTGTATTGCCCGCATGCACAGACTCCTGCTCACCCTCCTGCTCCTAATCCCCCTGCCAACCCACGCCCGCGCGGGCTAATTCTTTCTCCCCCCCAACCCCGCCGAGCTGGCCGGCCTGCTCGAAGGCt < 4:90923/145‑1 (MQ=255)
agaaTCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCt < 2:65517/147‑1 (MQ=255)
ccAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCGGCTCGAAGGCGGGgcag > 8:353370/1‑148 (MQ=255)
cAACACCCATTTCCCGCATTGCCCGCATCCTCCGACTCCTGCTCACCCTCCTGCTGCTAACCCCCCTGGCAACCCACGCCCGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCAGGCCGGCCTGCTCGAAGGCTGGgcagca < 2:360115/149‑1 (MQ=255)
acCTATTTCCTGTATTGCCCGCATGCTCACACTCCGCCTCACCCTCCTGCTGCAAAACCCCCTGCCAACCCACCCCAGCGTGGGCTAATTCTTTCACACCGCCAACCCCGCCGAGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACAAc < 4:60557/149‑1 (MQ=255)
acCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCGCGAAGGCTGGGCAGCACAAc > 1:52472/1‑149 (MQ=255)
cccGCATGATCAGACTACTGCTCAACCTCCTCCTGCTAATCCCCCTGGCAACCAACGCCAGCGAGGGCGAATTCTTTCTCCCCGCCAACCCCGCCCCCCAGGCCCGCCTGGAACAAGGGAGGGCAGACCCACCCCATGCCCCGCGccag > 2:104359/1‑147 (MQ=255)
ccGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggc < 2:250525/102‑1 (MQ=255)
ccGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggc < 2:256072/102‑1 (MQ=255)
ccGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggc > 1:256072/1‑102 (MQ=255)
ccGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggc > 1:250525/1‑102 (MQ=255)
tCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGAAGGCTGGGGAGCACAACCCCAAGCCGCCCGCCGGCCCCGGCTGGaaaa > 1:103883/1‑148 (MQ=255)
cccacccaccTGCTCAAAACCCCCCTCCCACCCCCCGCCGGCGGGGTCTATTTTTTTCACCCCCCAACCCCCGCCGAGCTGGCGGGCCGGCTCGAAGACTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTgc < 4:39375/146‑1 (MQ=255)
cccACCCTCCTGCTCCAAACCCCCCCGGCACCCCCCGCCCGCGCGGGCGAATTCTTTCCCCCCCCCCCCCCCGCCGCGCCGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTg < 1:261468/145‑1 (MQ=255)
cTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCTAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTgc < 8:196838/148‑1 (MQ=255)
tCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCGGCGCCAACGCTGGGCAGCCCAACCCCCAGCCCCGCGCCTGCCGCAGCTGGAAAACCAgcgc > 1:26846/1‑149 (MQ=255)
aCCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGGGCGCAGGCGGGGGCGCCCCACCCCCAGCCCCGCCCCCGCCGCGGCGGGAAAACCAGCGcc > 1:166360/1‑148 (MQ=255)
aCCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCCGCCGGCGCGAAGGCTGGGCAGCACAACCCCAAGCCCCGCGCCAGCCGCGGCTGGAAAACCACCGcc > 4:371043/1‑148 (MQ=255)
aCCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCACGCCCGCCGGCACGAAGGCTGGGCCGCCCAACCCGAATCCCCGCGCCTGCCCCGGCTGGAAAACCATCGcc > 2:8678/1‑148 (MQ=255)
cccTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCCGCCGGCGCGCAGGCTGGGGCGCACCACCCCCTGCCCCCCGCCTGCCGCGGCTGGGAAACCAGCGcc > 5:339398/1‑147 (MQ=255)
cctGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCCGGGCGAAGGCTGGGGAGCCCCACCCCCAGCCGCCCGCCAGCCGCGGCGGGCAAAACAGCGCCAAGGcc > 2:143633/1‑149 (MQ=255)
tgctAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCTGCGCGAAGGCTGGGCCGCACAACCCCAAGCCGCGCGCCTGCCGCTGCTGGAAAACCAGCGCCAAGGCCGCATc > 3:210324/1‑149 (MQ=255)
aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGcca < 2:52472/148‑1 (MQ=255)
ccccTGCCAACCCACGCCAGCGAGGGAGAATTCTTTCTCACCGCCAACCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGccacca < 8:246412/148‑1 (MQ=255)
acccccCGCCAGCGTTGGCTAATTCTTTCTCCCCGCCACGCCCGCCGCGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAg < 5:239113/146‑1 (MQ=255)
ccACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCAGCTGGAAAACCAGCGCCAAGGCCGCCTCGCCCCCAATGACAAgcgc > 2:101430/1‑148 (MQ=255)
cccGCCAGCGTGGGAGAATTCTTTCTCCCCCCCAACCCCGCCGAGCAGCCGGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCa < 7:311196/146‑1 (MQ=255)
gCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCCAAGCCCCGCGCCTGCCGCTGCTGGAAAACCAGCGCCAAGGCCGCATCGCCACCAAAGACAAGCGCAAGGTGAGCCGGaa > 2:209102/1‑148 (MQ=255)
attattTCTCCCCCCCACGCCCGCCGAGCTGGCGGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGaacaac < 2:103883/148‑1 (MQ=255)
ttctCACCGCCAAGCCCGCCGAGCAGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCCATGCCCCGCGCCAGCCGCTGCTGGAAAAACAGCGCCAAGGCCCCATCGCCCCCAATGACAAACGCAAGGTGCGCCTGAACAAcccc > 8:139306/1‑144 (MQ=255)
tctcACCGCCAAGCCCGCCGAGCAGGCCGGCCAGCTCGAAGGCTGGGCCGCACAACCCCAATCCGCGCCCCAGCCGCTGCTGGAAAACCAGCGCCAAAGCCCCATCGCCCCCAAATACAACCGCAAGGTGCGCCTGAACAACCCCCTgc > 2:209507/1‑149 (MQ=255)
cACCGCCAAGCCCGCCGAGCCCGCCGGCCGGCTCGCAAGCTGGGCAGCACAAACCCAAGCCCCCCCCCAGCCCCAGCGGGTAAACCAGCGCCCAAGCCGCAAAGCCCCCAAAGAAAAACCCAAAGTGCGCCTGAACAACccccgacgc > 6:252087/1‑143 (MQ=255)
cccccACGCCCGCCGAGCCGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCgg < 5:252087/145‑1 (MQ=255)
ccGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCgg > 6:163240/1‑148 (MQ=255)
cccGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGCTGCCGCGCCCCTGCCGCTGCTGGAAAACCAGCGCCAAGGCCGCCTCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCAGCGCGGCCTGa > 8:139949/1‑145 (MQ=255)
cccccgAGCCGGCGGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGAc < 2:340708/146‑1 (MQ=255)
tcgAGCAGGCCGGCCTGCTAGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACACCCGCCTGCGCGGCCTGATCGACaa > 6:197264/2‑148 (MQ=255)
gCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGccc > 4:345595/1‑149 (MQ=255)
|
GAGCGGGCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCCC > NC_002947_CJ‑RC/5512567‑5512857
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
