Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F14 I0 R1 206 76.7 3255580 98.8% 3216513 146.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATC  >  NC_002947_CJ‑RC/1932543‑1932788
                                                                                                  |                                                                                                                                                    
aaaaaaaatccaataacccgggggggcggtttgggccccggggggggcccccccccaacaaaaaatttttggcccccccgttttggcggggggtgccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCC                                                                                                  <  NB501016:282:H2V7GAFX2:3:21603:4968:14613/54‑1 (MQ=60)
             caccccgcgggggccggtggggggcccgcggcggccacccccccacaaaaacagtatcggccccccgggtcggggggggcgcccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGT                                                                                     <  NB501016:282:H2V7GAFX2:3:11611:7518:14130/67‑1 (MQ=60)
                  gggggggccggtttggggcccggggggggcacccccccacaaaaaaacttttcgccccccctttgcggggggggcccccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGC                                                                                <  NB501016:282:H2V7GAFX2:2:21311:17924:11950/72‑1 (MQ=60)
                                 gcgcccggggggggccacccccccccaaaaaatttttcgccgccccttttgggggggggtggccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAG                                                                 <  NB501016:282:H2V7GAFX2:1:21201:11002:10904/87‑1 (MQ=60)
                                             GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTT                                                     >  NB501016:282:H2V7GAFX2:3:21510:5499:3264/1‑151 (MQ=60)
                                              GGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTT                                                    >  NB501016:282:H2V7GAFX2:3:21501:6541:5503/1‑151 (MQ=60)
                                                    CCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCT                                              >  NB501016:282:H2V7GAFX2:2:11304:19227:2959/1‑151 (MQ=60)
                                                    CCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCT                                              >  NB501016:282:H2V7GAFX2:3:21412:14981:12049/1‑151 (MQ=60)
                                                     CCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCACGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTACAGCTC                                             >  NB501016:282:H2V7GAFX2:2:21105:12974:6441/1‑151 (MQ=60)
                                                         accacccgcccacccccccccggcgcggggggccccccccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATA                                         <  NB501016:282:H2V7GAFX2:2:21105:5427:6084/111‑1 (MQ=60)
                                                             ACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGC                                      >  NB501016:282:H2V7GAFX2:4:11409:10991:5169/1‑150 (MQ=60)
                                                               AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACA                                   <  NB501016:282:H2V7GAFX2:2:11111:8712:16017/151‑1 (MQ=60)
                                                                         cgccccgtgtggggggggggccccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGC                         <  NB501016:282:H2V7GAFX2:1:11106:15303:14702/127‑1 (MQ=60)
                                                                                  ggccggggggcgcccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA                <  NB501016:282:H2V7GAFX2:1:11206:11655:6154/136‑1 (MQ=60)
                                                                                           GCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGC       >  NB501016:282:H2V7GAFX2:1:11110:22765:4579/1‑151 (MQ=60)
                                                                                            gtcccCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCG      <  NB501016:282:H2V7GAFX2:4:21508:6183:12774/146‑1 (MQ=60)
                                                                                            CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCG      >  NB501016:282:H2V7GAFX2:2:21108:6090:12357/1‑151 (MQ=60)
                                                                                            CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATAAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATA                                         >  NB501016:282:H2V7GAFX2:1:21203:26018:2845/1‑116 (MQ=60)
                                                                                               C‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATC  >  NB501016:282:H2V7GAFX2:3:11605:2103:9314/1‑151 (MQ=60)
                                                                                                  |                                                                                                                                                    
CACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATC  >  NC_002947_CJ‑RC/1932543‑1932788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑