Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F29 I1 R1
|
57 |
65.8 |
2790598 |
98.6% |
2751529 |
146.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,499,498 |
1 bp→CG |
intergenic (+75/‑89) |
trpS → / → zapE |
tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,499,497 | 1 | . | C | 92.9%
| 38.9
/ ‑3.2
| 14 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (9/4); total (9/5) |
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_002947_CJ‑RC | 1,499,498 | 0 | T | G | 92.9%
| 40.2
/ NA
| 14 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (9/4); total (9/5) |
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TC‑‑GGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGC > NC_002947_CJ‑RC/1499358‑1499645
||
aaCGCTGCAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCTTTGCCGGTGTTCGC‑‑GGGCAc < 3:237969/149‑1 (MQ=37)
aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCTTTGCCGGTGTTCGC‑‑GGGCAc < 3:241470/149‑1 (MQ=37)
aaCGAAGCGTTGCCACCACAGCTGGTCGTCGGCTGCAAAAGGGTGGGCGCGGGGGTGCCCGCTAAACCGGCGCGGCGGGGAATGCCACCGGCTTTGCGGGTGTTCGC‑‑GGCCACG‑CGCGCT‑CCACAGACTGATTGCCATTAAGCGGGc < 4:247414/147‑1 (MQ=255)
gcgggcggGCCCGCTAAACCGGCCCCGGGGCGCATGG‑CACCGGGTTGGCGGTGGT‑TCGGGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:203460/149‑1 (MQ=255)
cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:100926/1‑148 (MQ=37)
gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 8:181628/1‑148 (MQ=37)
aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 2:51093/1‑149 (MQ=37)
ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 8:50793/1‑148 (MQ=37)
ggTGCATGGCACCGGCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGGTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 5:245414/1‑144 (MQ=37)
cGGCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc > 3:230088/1‑149 (MQ=39)
gCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 3:123343/90‑1 (MQ=25)
gCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg > 4:123343/1‑90 (MQ=25)
gCTTTGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa > 3:191055/1‑149 (MQ=39)
tttGCCGGTGTTCGC‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 1:54684/1‑149 (MQ=39)
gc‑‑GGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgc > 5:213633/2‑149 (MQ=255)
||
AACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TC‑‑GGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGC > NC_002947_CJ‑RC/1499358‑1499645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
