Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 59 79.3 3391134 98.6% 3343658 146.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 4,741,341 C→T intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,741,3410CT100.0% 149.3 / NA 45intergenic (+471/+282)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/23);  total (22/23)

TCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTG‑AACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947_CJ‑RC/4741229‑4741471
                                                                                                                |                                                                                                                                    
tCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGcccc                                                                                                 >  2:203351/1‑149 (MQ=255)
  cGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCg                                                                                                >  7:20311/1‑148 (MQ=255)
    cctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCggac                                                                                             <  1:419825/149‑4 (MQ=255)
             tGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGtcctcc                                                                                    >  6:348616/1‑149 (MQ=255)
             tGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGtcctcc                                                                                    <  4:111770/149‑1 (MQ=255)
               gtagTAATGTGCAGCACGGAATCACGCAGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                  >  3:166430/4‑147 (MQ=255)
               gTCGTAATGTGCAGGACGGAATCTCGCGGCGTGACCGGCCATGGCGTATTGAACTGTGTATAGGTCCTGCTCTGGTCACCTTCGTGCTTGATCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGAACAGGCCCCGGACACGtcctcct                                                                                   <  7:431944/148‑2 (MQ=255)
               gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                   <  6:19149/148‑2 (MQ=255)
                   tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGc                                                                              <  2:109952/149‑1 (MQ=255)
                   tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGc                                                                              <  4:87201/149‑1 (MQ=255)
                       gtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcct                                                                          >  1:42785/1‑148 (MQ=255)
                            gcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctcgacc                                                                     >  1:337691/1‑143 (MQ=255)
                             caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctcgacct                                                                    >  4:84363/1‑142 (MQ=255)
                                      aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcg                                                           >  8:360702/1‑149 (MQ=255)
                                       cgcgGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcgg                                                          <  5:207806/149‑1 (MQ=255)
                                          ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                       >  4:65644/1‑149 (MQ=255)
                                            cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                     >  2:40703/1‑149 (MQ=255)
                                             gTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                    <  5:176575/149‑1 (MQ=255)
                                                aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGTCCTTGGCCAGCGGCGCCTTGa                                                  <  4:119758/148‑1 (MQ=255)
                                                aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                 <  7:161744/149‑1 (MQ=255)
                                                    gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             <  1:82458/149‑1 (MQ=255)
                                                      cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                           <  1:203351/149‑1 (MQ=255)
                                                      cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                           >  7:181418/1‑149 (MQ=255)
                                                      cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                           >  4:79790/1‑149 (MQ=255)
                                                            gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                     <  2:114276/149‑1 (MQ=255)
                                                             tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                    <  3:49364/149‑1 (MQ=255)
                                                             tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                    >  4:49364/1‑149 (MQ=255)
                                                                 gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                 <  5:69520/148‑1 (MQ=255)
                                                                   aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              >  3:105419/1‑149 (MQ=255)
                                                                   aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              <  5:64004/149‑1 (MQ=255)
                                                                   aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              >  1:368607/1‑149 (MQ=255)
                                                                   aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                              <  7:17844/149‑1 (MQ=255)
                                                                    cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                             >  2:56700/1‑149 (MQ=255)
                                                                       ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                           <  4:166430/148‑1 (MQ=255)
                                                                        gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGACtt                         >  7:245633/1‑149 (MQ=255)
                                                                           taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                       <  6:160594/148‑1 (MQ=255)
                                                                           taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTa                      <  1:400642/149‑1 (MQ=255)
                                                                             ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACt                    >  1:160538/1‑149 (MQ=255)
                                                                               tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                  <  3:65644/149‑1 (MQ=255)
                                                                               tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                  >  8:249304/1‑149 (MQ=255)
                                                                                ccAGCTCTGGTCACCTTCGAGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAAACCACGC‑ACAGCCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCTGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTgg                 <  6:334440/149‑1 (MQ=255)
                                                                                       tGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCg            <  7:167102/147‑1 (MQ=255)
                                                                                            aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt     >  5:134151/1‑149 (MQ=255)
                                                                                              cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa   >  4:44447/1‑149 (MQ=255)
                                                                                               ttCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTG‑AATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCACCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc  >  6:6089/1‑149 (MQ=255)
                                                                                                                |                                                                                                                                    
TCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTG‑AACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947_CJ‑RC/4741229‑4741471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A