Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 59 79.3 3391134 98.6% 3343658 146.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGC  >  NC_002947_CJ‑RC/3447153‑3447425
                                                                                                                                          |                                                                                                                                      
CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGG                                                                                                                            >  NB501016:282:H2V7GAFX2:3:21611:2542:15789/1‑151 (MQ=60)
     ACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCCGC                                                                                                                       >  NB501016:282:H2V7GAFX2:3:11408:16671:16725/1‑151 (MQ=60)
                   gcttttgcttgcggtcgtcgtgtggtcttaccctctctaccccggcgctggcgaggtggggctgggcgggctggggggaccggcctggtggggggggcggtgcggggGGCCGCCTGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGG                                                                                                         <  NB501016:282:H2V7GAFX2:2:11101:17764:10918/44‑1 (MQ=60)
                    ggggcgcccctcgggctggccgcgcgccacgccctctacacccgcgcgctcgcggggctgcagggcgcggTTGGGCCACCCGCCGGGGCGCCGGGGCGCGGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGG                                                                                                         <  NB501016:282:H2V7GAFX2:1:21210:17451:7350/80‑1 (MQ=51)
                    ggggccagcaccggtcgggcgccgcccccggccctctacccgcgcgcgctcgcggggctgcagtgcgcgcgtgggccccccgccggggcgggggGGCGCTGCGGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGG                                                                                                         <  NB501016:282:H2V7GAFX2:1:11108:23929:4607/56‑1 (MQ=42)
                       GGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA                                                                                                      >  NB501016:282:H2V7GAFX2:2:11208:8220:12495/1‑150 (MQ=60)
                       GGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCCGGCCGCCGGGGTGTGGCAGGCCA                                                                                                      >  NB501016:282:H2V7GAFX2:4:21603:22667:11598/1‑150 (MQ=60)
                          AGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACCC                                                                                                  >  NB501016:282:H2V7GAFX2:2:21307:26179:5023/1‑151 (MQ=60)
                                gttctggagtctcgcacccccctctacccgcgcgggcgggaggggctgccgtgcgcgggggggccccccgcgggggcggCGTGGCGGTGCTGCGGGCCGCGAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAA                                                                                            <  NB501016:282:H2V7GAFX2:3:11407:10136:14705/72‑1 (MQ=60)
                                     ggagcaccgcacccccctctacccgcgcgcgcgggtggtgctgctgtgtgcgcttgccccccccgcctgggcgggggGGCGCTGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGG                                                                                          <  NB501016:282:H2V7GAFX2:2:21207:11335:16523/71‑1 (MQ=60)
                                     GGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCAGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCAACCCCGATAAGGCGC                                                                                       >  NB501016:282:H2V7GAFX2:4:21605:17948:10507/1‑151 (MQ=60)
                                                AGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCAACGCCTATAATGCGCAGCTCGCTGAT                                                                            >  NB501016:282:H2V7GAFX2:2:21307:17130:1682/1‑151 (MQ=60)
                                                   cacaCTACATGCGAGCGCTCGATGTGCTGCCGTGCGCGCTTGGGCCACGGGGCTGGTCGCCGTGGCGCTGCGGCTGGCCGCCAGCGCCGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAG                                                                         <  NB501016:282:H2V7GAFX2:2:21302:26276:3984/147‑1 (MQ=60)
                                                    CCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACCCCGATAAGGCGCAGCGCGCTGATCAGG                                                                        >  NB501016:282:H2V7GAFX2:2:11102:8161:7719/1‑151 (MQ=60)
                                                       caccccgcgcgggcgcggggggctgctgtgcgggggtgggccccccgggggggcgggggggcgcggcggcgGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCG                                                                     <  NB501016:282:H2V7GAFX2:2:11105:12191:18001/80‑1 (MQ=60)
                                                       CTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCCGGCACCCGGGGGGTGGCAGGCCAACGCCGAAAAGGCGCAGCTCGCTGATCAGcccc                                                                     >  NB501016:282:H2V7GAFX2:3:11412:10990:16308/1‑147 (MQ=60)
                                                                AGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCGATAATGCGCAGCTCGCTGATCAGGCCGATGACTTCG                                                            >  NB501016:282:H2V7GAFX2:3:21508:7790:4500/1‑151 (MQ=60)
                                                                 gcggtcgtggggttggagggtgggggtggcccaccggcggggtcggggggggGCTGCTGCTGGGCGCCAGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGG                                                           <  NB501016:282:H2V7GAFX2:3:21505:2227:5408/99‑1 (MQ=60)
                                                                      ggggggGCTGCAGTGCGCGCGGGGGCCCCCCGCCGGGGCGCCGGGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGC                                                      <  NB501016:282:H2V7GAFX2:3:11405:5080:14917/145‑1 (MQ=60)
                                                                             CTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGG                                               >  NB501016:282:H2V7GAFX2:2:21311:10981:5754/1‑151 (MQ=60)
                                                                              TGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCACGCAGTTGGCG                                              >  NB501016:282:H2V7GAFX2:1:11205:5566:6347/1‑151 (MQ=60)
                                                                                   ggtgcgctgggccccCCCGCCTGGGCGCGGTGGCGGTGCGGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAA                                         <  NB501016:282:H2V7GAFX2:2:11101:3995:7700/136‑1 (MQ=60)
                                                                                   gctctgcggGGGCCCCCCGCCTGGGCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAA                                         <  NB501016:282:H2V7GAFX2:3:11410:14869:8445/142‑1 (MQ=60)
                                                                                     cgcgcgtggcccccccgcgtgggcggggtggggcggctgcggcCCGCCTGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGG                                       <  NB501016:282:H2V7GAFX2:4:11605:17864:20289/108‑1 (MQ=60)
                                                                                     AGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCGGGCACCCTCGGTGTGGCAGGCCCACGCCAAAAAAACGCAGCTCGCAGATCAGGCCCGAGACAACTGCAACCAGTTGGCGGAGAAGG                                       >  NB501016:282:H2V7GAFX2:3:21403:7096:11013/1‑151 (MQ=60)
                                                                                            ggcccccccggcggggcgggggggcgctgggGCTGGCCGCCTGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGC                                <  NB501016:282:H2V7GAFX2:4:11611:23469:5126/120‑1 (MQ=60)
                                                                                             GGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTCGCGGATCAGGCCGATGGCTTCGGCAAGCAGTTGGGGGAGAAGGATCGCGCG                               >  NB501016:282:H2V7GAFX2:3:21611:17256:6712/1‑151 (MQ=60)
                                                                                                  CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCCACGCCAATAAGGCGCAGCTCGCGGATCAGGCCGATGACTTCGGGACGCAGTTGGCGGAGAAGGATCGCGCGCCCGG                          >  NB501016:282:H2V7GAFX2:3:21609:1626:14102/1‑151 (MQ=60)
                                                                                                     gggtgggggggggggggcgggggcgGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCT                       <  NB501016:282:H2V7GAFX2:4:21606:18334:6872/126‑1 (MQ=60)
                                                                                                      ggtggggggggggggGCTGCTGCGGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTG                      <  NB501016:282:H2V7GAFX2:3:11608:11976:13615/136‑1 (MQ=60)
                                                                                                            ggggggGGCGCTGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG                <  NB501016:282:H2V7GAFX2:3:11511:10355:5495/145‑1 (MQ=60)
                                                                                                               ggtggcgcggcggcggcCCGCCAGGGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAG             <  NB501016:282:H2V7GAFX2:2:11309:23418:16381/134‑1 (MQ=60)
                                                                                                                GTGGCGGGGCTGCGGGCCGCCTGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGA            <  NB501016:282:H2V7GAFX2:4:11608:19579:8377/151‑1 (MQ=60)
                                                                                                                  GGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGCAGGCCAACGCCGATAAGCCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGGGGAGAAGGCTCGCGCGCACGGggtggataagatgtgg          >  NB501016:282:H2V7GAFX2:4:21410:23359:14844/1‑135 (MQ=60)
                                                                                                                        GCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTTGCGGAGAAGGATCGCGCGCCCGGTCTGGAGCGGGAGAAGGCTGC     >  NB501016:282:H2V7GAFX2:4:11511:11658:5751/1‑150 (MQ=60)
                                                                                                                          TGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGC  >  NB501016:282:H2V7GAFX2:1:21107:8802:13001/1‑151 (MQ=60)
                                                                                                                                    CAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAG          <  NB501016:282:H2V7GAFX2:3:21612:11748:19175/133‑1 (MQ=60)
                                                                                                                                          |                                                                                                                                      
CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGC  >  NC_002947_CJ‑RC/3447153‑3447425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑