Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F14 I0 R1
|
248 |
61.6 |
2601574 |
98.7% |
2567753 |
146.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
4,946,950 |
G→A |
30.9% |
intergenic (+607/+191) |
PP_5673 → / ← flhB |
D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 4,946,950 | 0 | G | A | 30.9%
| 56.5
/ 29.3
| 42 | intergenic (+607/+191) | PP_5673/flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Reads supporting (aligned to +/- strand): ref base G (12/17); new base A (3/10); total (15/27) |
| Fisher's exact test for biased strand distribution p-value = 3.14e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01 |
CGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCC > NC_002947_CJ‑RC/4946806‑4947089
|
cGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTa < 1:67886/148‑1 (MQ=255)
gCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGAGCCGCCCGCGCGGCGCATCGAGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTAtta > 7:186537/1‑148 (MQ=255)
gCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGc < 5:14639/149‑1 (MQ=255)
ttGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCgg > 1:30202/1‑148 (MQ=255)
gAAGCTTTCCAACTTTGTGGTCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTa < 8:305250/148‑1 (MQ=255)
aggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTgcgc < 4:295723‑M2/53‑1 (MQ=255)
gtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTgcgcgc < 1:237146‑M2/55‑1 (MQ=255)
gttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGa > 2:246236‑M2/93‑149 (MQ=255)
ttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGAc > 2:13009‑M2/92‑149 (MQ=255)
ccAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGAcc < 1:232281/149‑1 (MQ=255)
ggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCcttg < 2:85289‑M2/65‑2 (MQ=255)
ggggattttcagcgcctgggaggtgtgcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCcttg < 5:137023‑M2/65‑2 (MQ=255)
gggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGccttgg > 5:100091‑M2/84‑147 (MQ=255)
ggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCgccttggg < 5:223532‑M2/67‑4 (MQ=255)
tgATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTcgc < 8:277661/148‑1 (MQ=255)
tgATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTcgc < 5:121981/148‑1 (MQ=255)
tgATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTcgc < 5:117085/148‑1 (MQ=255)
ttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCgaccgccttggggcat < 1:13009‑M2/71‑8 (MQ=255)
ttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCgaccgccttggggcat < 6:100091‑M2/71‑8 (MQ=255)
ttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCgaccgccttggggcat < 2:271339‑M2/71‑8 (MQ=255)
gctgTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACAcc > 2:180185/1‑149 (MQ=255)
ctgTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTa < 1:87682/144‑1 (MQ=255)
aaTCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGata < 7:79175/149‑1 (MQ=255)
gggTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTgcg < 6:207129/149‑1 (MQ=255)
ttCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCg < 5:119904/102‑1 (MQ=255)
ttCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCg > 6:119904/1‑102 (MQ=255)
cgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTac < 4:146378‑M2/91‑1 (MQ=255)
cgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcggGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTac < 2:153807‑M2/91‑1 (MQ=255)
tttgttttttGTCGCCTGTGATATGGTGCGCCGCCGGCGCGGCGCTTCGCGAGCAAGCTCGCTCCAACTTTTGTTTCGGGCCAGTTATTGCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTacaaacaa < 4:6779/144‑1 (MQ=25)
ggTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAgg < 2:235394/148‑1 (MQ=255)
gTTTTTGTCGCCTGTGAGATCGAGCGCGGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGTGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCg < 7:310290/149‑1 (MQ=255)
tttttGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCgg < 1:180185/149‑1 (MQ=255)
tttGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTc > 1:175576/1‑149 (MQ=255)
gTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTcgc < 4:290394/148‑1 (MQ=255)
tcgctcCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGgagcgagc > 2:61004/1‑149 (MQ=255)
ctcCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTg > 1:195018/1‑149 (MQ=255)
ccTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCt > 6:162826/1‑149 (MQ=255)
cTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTacaaacaa < 7:163650/85‑1 (MQ=255)
cTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTacaaacaa > 8:163650/1‑85 (MQ=255)
cTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTc > 7:60836/1‑149 (MQ=255)
aTTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGa > 8:329096/1‑149 (MQ=255)
ttCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGcgcc > 8:250331/1‑149 (MQ=255)
|
CGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCC > NC_002947_CJ‑RC/4946806‑4947089
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
