Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F81 I1 R1 61 77.8 3507706 98.1% 3441059 138.7

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCCCTGGCCAGCC  >  NC_002947_CJ‑RC/5512578‑5512866
                                                                                                                                              |                                                                                                                                                  
CCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCGGGCCGGCCGGCTCGC                                                                                                                                              >  NB501016:282:H2V7GAFX2:2:11211:22887:18902/1‑149 (MQ=60)
                CACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGGAGGCCGGCCTGCGCGAAGGCTGGGGAGCACAACC                                                                                                                            >  NB501016:282:H2V7GAFX2:3:11412:13153:8044/1‑151 (MQ=60)
                 ccctttttcctgtattccccccatcctcatactcctgccccccctcctgctcctaacccccctcccaccccccccccgcgtgggctAATTCTTTCTCCCCCCCAAGCCCGCCGAGCAGGCGGGCCTGCGCGAAGGCTGGGCAGCACAACCC                                                                                                                           <  NB501016:282:H2V7GAFX2:2:21202:16609:7211/65‑1 (MQ=60)
                                TGCCCGCATGCTCAGACTCCTGACCCCCCTCCTGCTGCTAACCCCCCCCCCAACCCACGCCTGCGAGGGCTAATTCTTTCACACCCCCCCGCCCGCCGCGCCGGGCGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCT                                                                                                             <  NB501016:282:H2V7GAFX2:1:21305:14856:12933/150‑1 (MQ=60)
                                          ccccgcaatccttcttttctgaatgggcctgttgttccgtggtgggctcttcctttctgctaattctttcacccccccacGCCCGCCGCGCCGGCGGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGG                                                                                                  <  NB501016:282:H2V7GAFX2:4:21505:3062:7429/71‑1 (MQ=60)
                                          CTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCACGCCCGCCGGCGCGAAGGCGGGGCCGCACAACCCcaagccccccgccagccgcagcggg                                                                                                  >  NB501016:282:H2V7GAFX2:4:21506:21858:9984/1‑126 (MQ=60)
                                            ccgcctcctccccccccccctgccccacagccccccgcccccccccccccgcgtgctcttattccctcccccccccccgcccgccgcgccgggcccCCTGCCCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGG                                                                                                  <  NB501016:282:H2V7GAFX2:1:11304:20046:11093/53‑1 (MQ=60)
                                             aatgtgactggagttcagccgtgtgctcatcccatcccacCCCACGCCAGCGAGGGCGAATTCTTTCCCCCCCCCCCCCCCGCCGCGCCGGCCGGCCGGCCCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAA                                                                                               <  NB501016:282:H2V7GAFX2:2:11305:14236:1878/111‑1 (MQ=60)
                                                        CACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGGCGGCCGGCGCGAAGGCTGGGGCGCACAACCCcctgacgcgcgccggccccggctggaaaaaaagcccca                                                                                     >  NB501016:282:H2V7GAFX2:1:11110:8069:17675/1‑112 (MQ=60)
                                                          ccctcctgctccaaccccccctgccaccccccccccgcgcgggctatttctttcccacccccaccccccccgcgccGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGG                                                                                  <  NB501016:282:H2V7GAFX2:3:11507:22628:3463/75‑1 (MQ=60)
                                                                       aacccccctcccacCCCACGCCAGCGCGGGCTAATTCTTTCCCCCCCCCCCGCCCGCCGACCAGGCCGCCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACC                                                                     <  NB501016:282:H2V7GAFX2:1:21103:5984:10359/137‑1 (MQ=60)
                                                                          CCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGAcggcgggggggcccaccccccagccgcgcgccggccgcggcggagataggaagagcacacggcagaactccactc                                                                   >  NB501016:282:H2V7GAFX2:4:11510:4010:6186/1‑75 (MQ=60)
                                                                           cccctcccacccccCGCCAGCGAGGGCTAATTCTTTCACACCGCCCAGCCCGCCGAGCCGGCCGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATG                                                                 <  NB501016:282:H2V7GAFX2:1:11311:13775:18230/137‑1 (MQ=60)
                                                                                      cctcgcccgcgagttagaattcttaccccccccccaccccgccgccccGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAG                                                      <  NB501016:282:H2V7GAFX2:1:21310:7377:2300/103‑1 (MQ=60)
                                                                                       CACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGGCGGCCGGCCGGCTCGCAGGCTGGGgggcccaacccccagccgcgcgcccgagatcggaagaagcacacctcggaactccagtcacaataagaaaaagaggttggg                                                     >  NB501016:282:H2V7GAFX2:1:11208:20580:15014/1‑70 (MQ=60)
                                                                                       CACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCGGCTCGAAGGCgggggggcacaacccccagccccgcgccggccgcggcggggaaaccagcgcccacaccgcagcgccaccaaagacaaccgccagg                                                     >  NB501016:282:H2V7GAFX2:4:11601:18440:12022/1‑66 (MQ=60)
                                                                                           CCAGCGAGGGCTAATTCTTTCTCACCGCCACGCCCGCCGAGCAGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCG                                                 <  NB501016:282:H2V7GAFX2:4:11406:10565:7860/151‑1 (MQ=60)
                                                                                                 acgtatatttctaccacacctcaaacccccctcAGCAGGCCTGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAA                                           <  NB501016:282:H2V7GAFX2:2:21306:21540:7439/118‑1 (MQ=60)
                                                                                                  GGGCGAATTCTCTCTCCCCGCCACCCCCGCCGAGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAA                                           <  NB501016:282:H2V7GAFX2:4:21412:3928:15521/150‑1 (MQ=60)
                                                                                                        ATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGAAGGCTGGGCAGCCCAACCCCATGCCGCGCGCCGGCCGCGGCGGGAAAACCTGCGCCAAGGCCGCCACGCCACCCAAGACAAGCCCAAGGTGCGCCTGAACAACCCC                                    >  NB501016:282:H2V7GAFX2:3:11502:9451:17938/1‑151 (MQ=60)
                                                                                                           cgagctccccgccccgcccgccccgccGGCCGGCCGGCCCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTG                                 <  NB501016:282:H2V7GAFX2:2:21312:22550:5936/124‑1 (MQ=60)
                                                                                                           CTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCGCGAAGGCGGGGGCGCCCCACCCCAAGCCGCCCGCCGGCCGCGGCGGGAAAACCAGCGCCCACGCCCCAGCGCCACCAAAAACAACCGCAAGGTGCGCCGGAACAACCCCC                                   >  NB501016:282:H2V7GAFX2:1:21105:14231:14634/1‑149 (MQ=13)
                                                                                                                   ccgccccgccggcggcGCAGGCCGGCCTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCT                         <  NB501016:282:H2V7GAFX2:4:21601:21783:19625/135‑1 (MQ=60)
                                                                                                                      CCACGCCCCCCGAGCCGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGAT                      <  NB501016:282:H2V7GAFX2:3:21604:19274:1278/151‑1 (MQ=60)
                                                                                                                       cccgcccgccgccCAGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATC                     <  NB501016:282:H2V7GAFX2:3:11411:12206:16304/138‑1 (MQ=60)
                                                                                                                           CCCGCCGACCCGGCCGCCCTCCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACA                 <  NB501016:282:H2V7GAFX2:3:11611:6551:11042/151‑1 (MQ=60)
                                                                                                                             CGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCAGCGCCAAGGCCCCAACGCCACCAATGACAACCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAAC               >  NB501016:282:H2V7GAFX2:4:21409:22244:14881/1‑151 (MQ=60)
                                                                                                                                 GAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACCACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCCTCGCCACCAATGACAAGCGCAAGGTGCGCCTGCACAACCGCCTGCGCGGCCTGATCGACAACGCCC           >  NB501016:282:H2V7GAFX2:2:11207:1306:1425/1‑151 (MQ=60)
                                                                                                                                  AGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCCCT          >  NB501016:282:H2V7GAFX2:4:21405:25573:9709/1‑151 (MQ=60)
                                                                                                                                          GGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCCCTGGCCAGCC  <  NB501016:282:H2V7GAFX2:1:11110:25360:10966/151‑1 (MQ=60)
                                                                                                                                              |                                                                                                                                                  
CCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAACGCCCTGGCCAGCC  >  NC_002947_CJ‑RC/5512578‑5512866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑