Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F18 I1 R1
|
59 |
77.7 |
3515218 |
98.5% |
3462489 |
145.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,126,647 |
+C |
intergenic (‑101/+48) |
tdcG‑II ← / ← gcvP‑I |
L‑serine dehydratase/glycine dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,126,645 | 1 | . | C | 95.7%
| 76.6
/ ‑3.0
| 23 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (15/7); total (15/8) |
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
CTGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑A‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947_CJ‑RC/1126497‑1126788
|
cTGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTaa‑a < 8:328900/149‑1 (MQ=255)
gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑A‑c < 6:299096/147‑1 (MQ=255)
gggCCTTTGCCGATCTTTAACAGGTCGAAGACGCTCAGTTACATGTGGACCTCCTGCCCGGTTGTTGGGTGGACATTGAGCCCTGTTGGCGCGGGTTTACCCG‑CTAATGCGGTGTTATAGCCACCATCGCATTCGGGGTTAA‑A‑cccgct < 2:405799/149‑5 (MQ=255)
gTCCAACACGCTCAGTCAATTGTCGACCTCCGGCCTGGTTGTGGGGGGCACTTTGCGCCCTTTCGGGGCGGTTTAACCCG‑CTATTGGGCCGTTATCCCCACCACCGCATCCGCGGTTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCaa < 2:44871/149‑1 (MQ=255)
ttgGGGGGACTTTCCGCCCTGTCGGCGGGGTTTTCCCGG‑CTATGGCGGCGGTATCCCCACCATCCAATGCGGGGTAAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctc < 6:162378/148‑1 (MQ=255)
ggggggACATTCCCCCCTGTCGGCGGGGGTTAACCCG‑CGAATGGGTAGTTATACCCACCATCGCATTCGGGGTAAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcg < 8:31971/149‑1 (MQ=255)
cccTGTCGGCG‑GGGGTTACCCGCCGACTGCGGCGTATTACCCACCACCCCATGCGCGGTAAACC‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGGGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 2:182/149‑1 (MQ=255)
gTTTACCCG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGCCGGGCAGGCGCACACCAGGTTGc > 3:95097/1‑149 (MQ=255)
gTTTACCCG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc > 3:158830/1‑149 (MQ=255)
ccG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 3:20189/108‑1 (MQ=255)
ccG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa > 4:20189/1‑108 (MQ=255)
ccG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCGGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 3:152804/1‑149 (MQ=255)
ccG‑CGAATGCGGAGGTATAGCCACCATCGCATTCCCCGGAAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGCCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 3:146519/1‑149 (MQ=255)
ggAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGtt < 1:385874/131‑1 (MQ=255)
ggAGGTATAGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGtt > 2:385874/1‑131 (MQ=255)
taGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 5:86466/1‑149 (MQ=255)
taGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 3:183224/1‑149 (MQ=255)
taGCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 7:301691/1‑149 (MQ=255)
gCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 3:378902/1‑149 (MQ=255)
gCCACCATCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCGCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 4:371562/1‑149 (MQ=255)
ccaccaTCCCATTCGGGGTTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg < 2:152317/149‑1 (MQ=255)
accaTCGCATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGACGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGcc > 1:154736/1‑149 (MQ=255)
cATTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGCCcggc > 4:58349/1‑149 (MQ=255)
aTTCGCGGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcg > 8:115429/1‑149 (MQ=255)
cgGGTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg < 3:371562/149‑1 (MQ=255)
gTAA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc > 3:348963/1‑149 (MQ=255)
|
CTGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCG‑CGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAA‑A‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947_CJ‑RC/1126497‑1126788
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCC > NC_002947_CJ‑RC/1126538‑1126793
|
agctcacgggggcccccccgccggggtttggggggcaaactccccccgggtcgggggggttcccccgcccacgcggctgtaacccccaccccccactgggggtaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGG < NB501016:282:H2V7GAFX2:2:21306:24409:7452/42‑1 (MQ=60)
gccactgcgccccccccgccgtgttgggggggcccaatcgcccccgggggggggggtttcccccggctttggggtttaaaccccccccccccctggggggtaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGG < NB501016:282:H2V7GAFX2:2:11209:8560:14804/42‑1 (MQ=60)
cgccgggggccccccccgccttgtgggggggggaaccccgcccccgtgggggggggggtcccccggaccgggggctttacccccccccccccctgggggttaaacccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAG < NB501016:282:H2V7GAFX2:4:21409:25097:4136/44‑1 (MQ=60)
ccctccgcccccccccccccgggggggggggcccccccccccccggggcgggggggtcccccccgccccgggccccacaccccccccccccctcgcgccccaaacccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAG < NB501016:282:H2V7GAFX2:4:21602:20735:7854/44‑1 (MQ=60)
caccccctccctgttggtgggggccaattccccccgtggcgggggggtataccccccaatggcgctgtaacccccaccagcccttcgggtttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGG < NB501016:282:H2V7GAFX2:4:21607:24230:9792/54‑1 (MQ=60)
catcgccccccgtgtggcggggggccccccggctccgcgcgcgtacccccccccccccctgcgggggaaacccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACG < NB501016:282:H2V7GAFX2:4:11604:22569:18796/78‑1 (MQ=60)
cccccgtgtcggggggggtcacccggctattgggtcttaacccccccaaccccttgggggttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGG < NB501016:282:H2V7GAFX2:3:21510:2076:12945/84‑1 (MQ=60)
cccctgtcgggggggtttccccggctttgggttttataaccccccccccgattgggggtaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCG < NB501016:282:H2V7GAFX2:4:11403:4404:13378/86‑1 (MQ=60)
ggcgtggtgggttcaccctgtcatggggggtcaaacccacccctctctttgggttaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCAC < NB501016:282:H2V7GAFX2:1:11305:26122:19335/89‑1 (MQ=60)
gcgggggcctccgcgtttgtgtctgtcattccccccgccgcttctgggttcaaacccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAG < NB501016:282:H2V7GAFX2:4:21607:15496:1181/94‑1 (MQ=60)
ggtttccccggttattgcgtctttaacccacccacctattggggtttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGC < NB501016:282:H2V7GAFX2:3:21605:5550:2434/99‑1 (MQ=60)
GGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGCCGGGCAGGCGCACACCAGGTTGCG > NB501016:282:H2V7GAFX2:2:11204:12298:16901/1‑151 (MQ=60)
GGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCG > NB501016:282:H2V7GAFX2:2:11303:15539:1732/1‑151 (MQ=60)
taccccgcattggggttttaaacccccccacccattggggtttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGT < NB501016:282:H2V7GAFX2:2:11312:20026:2571/102‑1 (MQ=60)
CCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAG < NB501016:282:H2V7GAFX2:2:11104:15230:9335/110‑1 (MQ=60)
CCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCGGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCC > NB501016:282:H2V7GAFX2:2:11302:26667:2891/1‑151 (MQ=60)
CCCGCGAATGCGGAGGTATAGCCACCATCGCATTCCCCGGAAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGCCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCC > NB501016:282:H2V7GAFX2:2:11301:1222:2908/1‑151 (MQ=60)
gttgttaaacccacctccctttggggtgtaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGTGGAGGGCTATCAGGCGTCCTTATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTG < NB501016:282:H2V7GAFX2:2:21307:24979:13088/116‑1 (MQ=60)
CGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTG < NB501016:282:H2V7GAFX2:1:21304:26362:5712/133‑1 (MQ=60)
tttaagcaaccatcgtttgcgggttaaaccgCGCTCCTACATAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGA < NB501016:282:H2V7GAFX2:1:11205:22883:17687/119‑1 (MQ=60)
tacccccccatagccaggggtgttaacccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAC < NB501016:282:H2V7GAFX2:4:21410:12244:15923/121‑1 (MQ=60)
aaccccccccccccctggggtttcaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACC < NB501016:282:H2V7GAFX2:2:21203:16258:12025/122‑1 (MQ=60)
ATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCC > NB501016:282:H2V7GAFX2:2:11306:1742:16938/1‑151 (MQ=60)
ATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCC > NB501016:282:H2V7GAFX2:3:11503:2244:7539/1‑151 (MQ=60)
ATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCC > NB501016:282:H2V7GAFX2:4:21412:12904:2508/1‑151 (MQ=60)
AGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGG > NB501016:282:H2V7GAFX2:2:21305:10536:13327/1‑151 (MQ=60)
AACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGACGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCC > NB501016:282:H2V7GAFX2:1:11301:13783:7579/1‑151 (MQ=60)
GCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGT < NB501016:282:H2V7GAFX2:2:21304:19898:9245/151‑1 (MQ=60)
GGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACT > NB501016:282:H2V7GAFX2:2:21212:18842:4528/1‑151 (MQ=60)
AAC‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCC > NB501016:282:H2V7GAFX2:4:11509:15413:17679/1‑151 (MQ=60)
|
CAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCC > NC_002947_CJ‑RC/1126538‑1126793
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
