Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F11 I1 R1 56 91.5 3907174 98.4% 3844659 146.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 4,741,403 A→C intergenic (+533/+220) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,741,4030AC100.0% 121.7 / NA 35intergenic (+533/+220)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/22);  total (13/22)

TCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCT‑CC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTT  >  NC_002947_CJ‑RC/4741265‑4741518
                                                                                                                                             |                                                                                                                   
tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAg                                                                                                            >  2:33453/1‑149 (MQ=255)
    gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                                                        <  4:214869/149‑1 (MQ=255)
       gcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGgggc                                                                                                      <  6:204154/148‑1 (MQ=255)
        cttCACCTGCCTTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTGGTTCTTGAGCAGCTTCTGCGAGTTGC‑ATTCGTTAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCTGCGCCt                                                                                                    <  7:221261/147‑1 (MQ=255)
         gTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                   <  3:465590/149‑1 (MQ=255)
             ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                                                >  8:292406/1‑148 (MQ=255)
              cGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGTTCa                                                                                              <  4:377795/149‑1 (MQ=255)
              cGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                                               >  5:86437/1‑148 (MQ=255)
                gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                            <  1:270179/149‑1 (MQ=255)
                gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                            <  7:417882/149‑1 (MQ=255)
                 ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                                           <  8:955/149‑1 (MQ=255)
                   aTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                                         <  8:406660/149‑1 (MQ=255)
                    tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACCCGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCATGATCACGGTc                                                                                         >  5:13793/1‑148 (MQ=255)
                        gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                                                    <  1:261970/149‑1 (MQ=255)
                             gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                <  7:117825/148‑1 (MQ=255)
                               aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                              <  5:453350/148‑1 (MQ=255)
                                   ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                           <  1:216251/147‑1 (MQ=255)
                                   ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                           <  2:351932/147‑1 (MQ=255)
                                    gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                                          <  6:350757/147‑1 (MQ=255)
                                     tttaGGTCCAGCTCTGGTCACCTTCGTGCTTGTG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTGCT‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg                                                                       <  2:414878/147‑1 (MQ=255)
                                       taGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTa                                                                     <  2:295198/149‑1 (MQ=255)
                                          gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                  >  2:272411/1‑149 (MQ=255)
                                          gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                  >  8:259210/1‑149 (MQ=255)
                                            ccAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                 <  5:388880/148‑1 (MQ=255)
                                              aGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc                                                              <  1:13353/149‑1 (MQ=255)
                                                ctctGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcc                                                             >  6:208803/1‑148 (MQ=255)
                                                          cTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                                                  >  8:491009/1‑149 (MQ=255)
                                                          cTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                                                  >  2:140002/1‑149 (MQ=255)
                                                                                  gacttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc                           <  7:167073/142‑1 (MQ=255)
                                                                                    cttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct                         >  8:32567/6‑149 (MQ=255)
                                                                                     ttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                        >  1:44780/5‑149 (MQ=255)
                                                                                       gcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                        <  7:259210/145‑1 (MQ=255)
                                                                                       gcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                        <  4:234286/145‑1 (MQ=255)
                                                                                                     aggcacaGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGc        >  7:269708/7‑149 (MQ=255)
                                                                                                           aGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGttt  >  3:268913/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                   
TCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCT‑CC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTT  >  NC_002947_CJ‑RC/4741265‑4741518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A