Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	57	69.7	2993452	98.5%	2948550	147.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947_CJ‑RC	4,768,005	T→A	intergenic (+112/‑70)	fpvA → / → tpaK	TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947_CJ‑RC	4,768,005	0	T	A	100.0%	281.1 / NA	85	intergenic (+112/‑70)	fpvA/tpaK	TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (36/49); total (36/49)

AACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAG  >  NC_002947_CJ‑RC/4767861‑4768143

aaCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAAt                                                                                                                                        <  4:289759/149‑1 (MQ=255)

  cTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAAtgt                                                                                                                                      >  6:85730/1‑149 (MQ=255)

   ttcaccttcaccGCCTAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAAtgtg                                                                                                                                     <  6:230397/149‑1 (MQ=25)

     cACCATCACCTCGAAGTACAGCTTGTGATCGCCTCACGTTGAAAGCAAAAAACCTCAAACTTGTGGGTTTTTTTGAATTCTCTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATAATCGGCTAGTATAATGTGAt                                                                                                                                   <  5:119689/149‑1 (MQ=17)

     cACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAAtgtg                                                                                                                                     <  8:71998/147‑1 (MQ=255)

      aCCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGAt                                                                                                                                   <  8:249862/148‑1 (MQ=255)

          tCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGAc                                                                                                                              >  8:23299/1‑149 (MQ=255)

            aCCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCAGTAGTAGTCCCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGAt                                                                                                                                   <  2:18390/142‑1 (MQ=25)

            aCCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCACTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGAt                                                                                                                                   >  1:18390/1‑142 (MQ=255)

             ccGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTg                                                                                                                           >  6:353976/1‑149 (MQ=255)

                ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGaa                                                                                                                        >  3:234990/1‑149 (MQ=255)

                        aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGc                                                                                                                >  6:141003/1‑149 (MQ=255)

                        aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGc                                                                                                                >  6:3200/1‑149 (MQ=255)

                        aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGc                                                                                                                <  1:295198/149‑1 (MQ=255)

                           ttCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGa                                                                                                             >  5:359737/1‑149 (MQ=255)

                            tCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATTATGTGATCAGACCTGGAATTGTGAGCGGa                                                                                                             >  7:327997/1‑148 (MQ=255)

                               gATCGCCTGACGTAGAACGCAAAAAACCGCACCCAGGTGCCGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCATGTATGATTACTATTGAAAATTAATCATCGGCTCGTATAATGTGATCAGACATGGAATTGTGAGCCGATaa                                                                                                          >  4:341899/1‑148 (MQ=18)

                                 tCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACaa                                                                                                       <  5:89382/149‑1 (MQ=255)

                                   gCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTATTAGTCTCTATGGAGGTCATGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAAt                                                                                                      <  5:115707/148‑1 (MQ=18)

                                   gCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAAtt                                                                                                     <  7:291704/149‑1 (MQ=255)

                                   gCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAAtt                                                                                                     >  4:220069/1‑149 (MQ=255)

                                       gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCATGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCtt                                                                                                  >  8:249277/1‑148 (MQ=255)

                                        aCGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGATTTGTGAGCGGATAACAATTCTTaa                                                                                                <  2:274849/149‑1 (MQ=255)

                                        aCGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTaa                                                                                                <  3:295871/149‑1 (MQ=255)

                                        aCGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTaa                                                                                                <  5:122303/149‑1 (MQ=255)

                                         cGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTTAATTCACTAGTAGTATCTATGGAGGTCATGTATGAATACTATTGACAATTAATCATCGGCTCGTATAATGTTATCAGACCTGGAATTGTGAGCGGATAACAATTCTTaa                                                                                                <  7:195272/148‑1 (MQ=17)

                                          gTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGa                                                                                              <  6:77576/149‑1 (MQ=255)

                                           ttGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGAt                                                                                             >  2:322330/1‑149 (MQ=255)

                                               aggCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAAc                                                                                         <  3:209144/147‑1 (MQ=255)

                                               aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGa                                                                                                                               >  8:291702/1‑111 (MQ=255)

                                               aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGa                                                                                                                               <  7:291702/111‑1 (MQ=255)

                                               aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTaa                                                                                          <  8:305904/148‑1 (MQ=255)

                                               aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAAc                                                                                         >  6:3287/1‑149 (MQ=255)

                                                  cAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTca                                                                                      >  8:154495/1‑149 (MQ=255)

                                                   aaaaaaCCGCACCTGGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTca                                                                                      >  6:245872/1‑148 (MQ=14)

                                                   aaaaaaCCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCg                                                                                                               >  5:15645/1‑123 (MQ=255)

                                                   aaaaaaCCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCg                                                                                                               <  6:15645/123‑1 (MQ=255)

                                                   aaaaaaCCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTca                                                                                      >  5:316780/1‑148 (MQ=255)

                                                     aaaaCCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTcacac                                                                                   <  7:185014/149‑1 (MQ=255)

                                                       aaCCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAg                                                                                 <  6:255143/149‑1 (MQ=255)

                                                        aCCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAgg                                                                                <  5:90881/149‑1 (MQ=255)

                                                         ccGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGTAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGga                                                                               <  7:287072/149‑1 (MQ=255)

                                                            cACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGgaga                                                                             >  1:135727/1‑148 (MQ=255)

                                                             aCCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGata                                                                           >  2:143003/1‑149 (MQ=255)

                                                               ccAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATc                                                                         <  5:85730/149‑1 (MQ=255)

                                                               ccAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTCTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGatat                                                                          >  4:309853/1‑148 (MQ=255)

                                                                   gTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCatat                                                                     <  5:356751/149‑1 (MQ=255)

                                                                        gTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATGACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCt                                                                <  5:314177/149‑1 (MQ=255)

                                                                                     aCTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATcgcg                                                   <  1:284166/149‑1 (MQ=255)

                                                                                         gtagtCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATGATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATGGCGCGt                                                <  2:162589/148‑1 (MQ=11)

                                                                                         gtagtCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGt                                                >  1:195213/1‑148 (MQ=255)

                                                                                         gtagtCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGt                                                <  7:244002/148‑1 (MQ=255)

                                                                                          tagtCTCTATGGAGGTCAGGGATGATTACTATTGACAATTACTCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGAAATCATATGTCCTTAGCACCATCGCGCGTGa                                              <  6:358953/149‑1 (MQ=14)

                                                                                           agtCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGa                                              <  1:143003/148‑1 (MQ=255)

                                                                                            gtCTCTATGGAGGTCAGGTTTTATTACTATAGTCAAATAATGATCGGGTCGTATATTGGGAGCAGACCTGGAATTGTTAGCGGATAACTATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGaga                                            <  2:362488/149‑3 (MQ=14)

                                                                                            gtCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGac                                             <  2:351259/148‑1 (MQ=255)

                                                                                               tctATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTg                                         >  1:64268/1‑149 (MQ=255)

                                                                                               tctATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTg                                         >  4:252457/1‑149 (MQ=255)

                                                                                               tctATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTg                                         >  2:43718/1‑149 (MQ=255)

                                                                                                  aTGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCg                                      >  8:93913/1‑149 (MQ=255)

                                                                                                   tGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCg                                      <  1:152090/148‑1 (MQ=255)

                                                                                                     gAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGa                                    <  8:53969/148‑1 (MQ=255)

                                                                                                        gTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGAttt                                 >  5:127983/1‑148 (MQ=255)

                                                                                                        gTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGAttt                                 >  2:345754/1‑148 (MQ=255)

                                                                                                        gTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTc                                <  5:3287/149‑1 (MQ=255)

                                                                                                           aGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCAt                              <  6:316780/148‑1 (MQ=255)

                                                                                                           aGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCAt                              <  1:43718/148‑1 (MQ=255)

                                                                                                           aGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATc                             >  2:183993/1‑149 (MQ=255)

                                                                                                              tATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGAt                          <  4:114815/149‑1 (MQ=255)

                                                                                                                  aTTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTcgc                      <  4:334783/149‑1 (MQ=255)

                                                                                                                         ttgaCAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCa                 <  2:366850/144‑1 (MQ=255)

                                                                                                                           gaCAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGt               >  8:309352/2‑147 (MQ=255)

                                                                                                                              aaTTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTTAGAGCGGATAACAATTCTTAAGATAAACTAACACAGGAGATATCATATGTCCTTAGCACCATAGCGCGTGACACTGCCGGATTTCATCGATTCGCGTCAATTGAGTc          >  5:120101/1‑149 (MQ=18)

                                                                                                                                ttAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGa        >  3:121625/1‑149 (MQ=255)

                                                                                                                                  aaTCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGAt       <  5:141003/148‑1 (MQ=255)

                                                                                                                                   atcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATTACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAc     <  4:322832/149‑1 (MQ=255)

                                                                                                                                   atcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATa      <  5:245872/148‑1 (MQ=255)

                                                                                                                                   atcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATa      <  5:353976/148‑1 (MQ=255)

                                                                                                                                   atcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAc     <  2:195213/149‑1 (MQ=255)

                                                                                                                                   atcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAc     <  1:10062/149‑1 (MQ=255)

                                                                                                                                   atcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGGGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAc     <  8:296015/149‑1 (MQ=255)

                                                                                                                                    tcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAcc    <  4:234990/149‑1 (MQ=255)

                                                                                                                                    tcatcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAc     >  4:187343/1‑148 (MQ=255)

                                                                                                                                     catcGGCTCGTAAAATGTGAACAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATAACATATGACCTTAGCACCAACGCGCGTGACGCTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAc     >  6:37415/1‑147 (MQ=21)

                                                                                                                                       tcGGCTCGTATAATGTGGTCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAg  >  7:308688/1‑148 (MQ=255)

AACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAG  >  NC_002947_CJ‑RC/4767861‑4768143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGATC  >  NC_002947_CJ‑RC/4767865‑4768155

ACACCATCACCTCGAAGTACAGCTTGTGATCGCCTCACGTTGAAAGCAAAAAACCTCAAACTTGTGGGTTTTTTTGAATTCTCTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATAATCGGCTAGTATAATGTGATC                                                                                                                                              <  NB501016:282:H2V7GAFX2:3:11511:3089:18270/151‑1 (MQ=0)

       CACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCACTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATC                                                                                                                                              >  NB501016:282:H2V7GAFX2:1:11104:7971:5132/1‑144 (MQ=60)

           GCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAAT                                                                                                                                   >  NB501016:282:H2V7GAFX2:2:21109:23006:14716/1‑151 (MQ=60)

                   CAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCG                                                                                                                           <  NB501016:282:H2V7GAFX2:1:21208:2439:16320/151‑1 (MQ=60)

                      CTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGAT                                                                                                                        >  NB501016:282:H2V7GAFX2:3:21609:3208:13780/1‑151 (MQ=60)

                       TTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATTATGTGATCAGACCTGGAATTGTGAGCGGAT                                                                                                                        >  NB501016:282:H2V7GAFX2:4:21602:21040:5728/1‑150 (MQ=60)

                            ATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAAA                                                                                                                  <  NB501016:282:H2V7GAFX2:3:11506:17780:6167/151‑1 (MQ=60)

                              CGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTATTAGTCTCTATGGAGGTCATGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATT                                                                                                                 <  NB501016:282:H2V7GAFX2:3:11511:9826:3900/150‑1 (MQ=60)

                              CGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTC                                                                                                                <  NB501016:282:H2V7GAFX2:4:21507:5975:9522/151‑1 (MQ=60)

                                   GACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAG                                                                                                           <  NB501016:282:H2V7GAFX2:3:11512:16896:8428/151‑1 (MQ=60)

                                   GACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAG                                                                                                           <  NB501016:282:H2V7GAFX2:2:21211:2584:11142/151‑1 (MQ=60)

                                    ACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTTAATTCACTAGTAGTATCTATGGAGGTCATGTATGAATACTATTGACAATTAATCATCGGCTCGTATAATGTTATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAG                                                                                                           <  NB501016:282:H2V7GAFX2:4:21401:26308:9701/150‑1 (MQ=43)

                                          AAGGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACT                                                                                                    <  NB501016:282:H2V7GAFX2:2:21104:3363:9672/151‑1 (MQ=41)

                                          AACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGAC                                                                                                                                          <  NB501016:282:H2V7GAFX2:4:21507:1805:9517/113‑1 (MQ=52)

                                              CAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCAC                                                                                                 >  NB501016:282:H2V7GAFX2:3:21601:24714:18120/1‑150 (MQ=40)

                                              CAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGG                                                                                                                          >  NB501016:282:H2V7GAFX2:3:11404:20673:1946/1‑125 (MQ=42)

                                                AAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACA                                                                                              <  NB501016:282:H2V7GAFX2:4:11611:2535:10708/151‑1 (MQ=36)

                                                   AACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGA                                                                                           <  NB501016:282:H2V7GAFX2:3:11506:12263:11368/151‑1 (MQ=30)

                                                    ACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGTAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAG                                                                                          <  NB501016:282:H2V7GAFX2:4:21506:5398:12348/151‑1 (MQ=27)

                                                       GCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGAT                                                                                        >  NB501016:282:H2V7GAFX2:1:11301:15193:18066/1‑150 (MQ=22)

                                                          CCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCA                                                                                    <  NB501016:282:H2V7GAFX2:3:11505:13943:12582/151‑1 (MQ=16)

                                                              GGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATG                                                                                <  NB501016:282:H2V7GAFX2:3:21609:12999:2367/151‑1 (MQ=5)

                                                                   GGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATGACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTT                                                                           <  NB501016:282:H2V7GAFX2:3:21601:3988:9439/151‑1 (MQ=5)

                                                                                CACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGC                                                              <  NB501016:282:H2V7GAFX2:1:21206:19848:13430/151‑1 (MQ=5)

                                                                                    AGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTG                                                           <  NB501016:282:H2V7GAFX2:4:21410:11313:10712/150‑1 (MQ=9)

                                                                                    AGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTG                                                           >  NB501016:282:H2V7GAFX2:1:21101:18322:12070/1‑150 (MQ=9)

                                                                                      TAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGAC                                                         <  NB501016:282:H2V7GAFX2:1:11303:8017:6939/150‑1 (MQ=16)

                                                                                          CTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGC                                                    >  NB501016:282:H2V7GAFX2:1:11112:17278:8840/1‑151 (MQ=26)

                                                                                              ATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGG                                                 <  NB501016:282:H2V7GAFX2:1:11305:11209:2166/150‑1 (MQ=32)

                                                                                                   GGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCA                                           <  NB501016:282:H2V7GAFX2:3:11401:2716:13109/151‑1 (MQ=44)

                                                                                                   GGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTC                                            >  NB501016:282:H2V7GAFX2:3:11601:23523:8627/1‑150 (MQ=42)

                                                                                                      CATGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATC                                         <  NB501016:282:H2V7GAFX2:1:11108:23889:13856/150‑1 (MQ=47)

                                                                                                                         CAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTTAGAGCGGATAACAATTCTTAAGATAAACTAACACAGGAGATATCATATGTCCTTAGCACCATAGCGCGTGACACTGCCGGATTTCATCGATTCGCGTCAATTGAGTCG                     >  NB501016:282:H2V7GAFX2:3:11511:5342:19730/1‑151 (MQ=60)

                                                                                                                           ATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGAT                   >  NB501016:282:H2V7GAFX2:2:11212:19033:7702/1‑151 (MQ=60)

                                                                                                                             TAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATA                  <  NB501016:282:H2V7GAFX2:3:11603:20827:13450/150‑1 (MQ=60)

                                                                                                                              AATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACC                <  NB501016:282:H2V7GAFX2:1:11102:21496:15373/151‑1 (MQ=60)

                                                                                                                              AATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAC                 <  NB501016:282:H2V7GAFX2:3:21608:19166:11884/150‑1 (MQ=60)

                                                                                                                              AATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATAC                 <  NB501016:282:H2V7GAFX2:3:21411:5473:19477/150‑1 (MQ=60)

                                                                                                                                  ATCGGCTCGTATAATGTGGTCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGT             >  NB501016:282:H2V7GAFX2:4:21510:24652:12125/1‑150 (MQ=60)

                                                                                                                                         CGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCG       <  NB501016:282:H2V7GAFX2:3:11401:26135:12722/149‑1 (MQ=60)

                                                                                                                                           TATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGA    <  NB501016:282:H2V7GAFX2:1:11311:17029:8094/150‑1 (MQ=60)

                                                                                                                                            ATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGATC  <  NB501016:282:H2V7GAFX2:3:11404:4652:11490/151‑1 (MQ=60)

                                                                                                                                            ATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGAT   <  NB501016:282:H2V7GAFX2:3:21511:7292:3281/150‑1 (MQ=60)

TCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGATC  >  NC_002947_CJ‑RC/4767865‑4768155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`