Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F54 I1 R1
|
60 |
79.7 |
3402854 |
98.4% |
3348408 |
145.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
4,809,883 |
2 bp→CG |
intergenic (‑289/‑265) |
PP_4234 ← / → dsbD‑II |
bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 4,809,883 | 0 | A | C | 76.9%
| 22.6
/ 4.0
| 13 | intergenic (‑289/‑266) | PP_4234/dsbD‑II | bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (2/8); total (4/9) |
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_002947_CJ‑RC | 4,809,884 | 0 | A | G | 76.9%
| 18.3
/ 2.0
| 13 | intergenic (‑290/‑265) | PP_4234/dsbD‑II | bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (2/8); total (4/9) |
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 |
ATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACC > NC_002947_CJ‑RC/4809741‑4810031
||
aTGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgct < 1:138636/149‑2 (MQ=255)
aTGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgct < 1:252950/149‑2 (MQ=255)
gCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGgcgcttcgc < 8:287848/149‑6 (MQ=255)
cGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg < 3:206040/149‑1 (MQ=255)
cGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCttcgcg < 3:264688/149‑1 (MQ=255)
ggAATTCGCGCTTTGGCGGGTTGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGGGTTCGCCGGGGGATTTTCAGCGCCTGGTAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg < 1:120155/149‑1 (MQ=37)
ggAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg < 5:423249/149‑1 (MQ=255)
aTTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgggt > 3:211121/1‑147 (MQ=255)
ggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg < 7:31676/84‑1 (MQ=18)
ggggATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCgcgg > 8:31676/1‑84 (MQ=18)
gtttacccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaa > 5:154862/6‑149 (MQ=255)
ccgcGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaacaaca > 7:222622/1‑148 (MQ=255)
gcgAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAg < 8:222622/149‑1 (MQ=255)
aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGAcc < 6:135825/148‑1 (MQ=255)
||
ATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACC > NC_002947_CJ‑RC/4809741‑4810031
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
