Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 54 84.9 3616994 98.4% 3559122 146.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 4,740,923 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,740,9230TG100.0% 90.7 / NA 27intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/18);  total (9/18)

TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATC  >  NC_002947_CJ‑RC/4740784‑4741038
                                                                                                                                           |                                                                                                                   
tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCg                                                                                                           >  3:212255/1‑149 (MQ=255)
  cAGCCCGTACTAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCg                                                                                                           <  2:356122/147‑1 (MQ=255)
  cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGc                                                                                                         <  1:374589/149‑1 (MQ=255)
  cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGc                                                                                                         <  4:206088/149‑1 (MQ=255)
   aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGcc                                                                                                        <  6:341482/149‑1 (MQ=255)
       cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCggggcggt                                                                                                    >  3:381950/1‑143 (MQ=255)
         tACAAGATCGGACGTCCGCGACAGCTGTACACCGGCGAGAAGAACCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGATCGCAAGAAGCCTGCCCTCGGGCAGCCTTTATTATTTGTCATGTGCCGGGctcatag                                                                                                  >  6:382649/1‑145 (MQ=255)
           cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                 <  4:12053/148‑1 (MQ=255)
           cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                 <  4:5942/148‑1 (MQ=255)
            aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTGGCgg                                                                                               >  7:385530/1‑149 (MQ=255)
              gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                             <  8:435284/149‑1 (MQ=255)
                      gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGGCGCATAGCGGGCCTGGCcgc                                                                                     >  4:193839/1‑149 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                  <  4:403312/149‑1 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                  <  4:187019/149‑1 (MQ=255)
                         cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                   <  4:212255/148‑1 (MQ=255)
                          gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGaa                                                                                  >  7:13151/1‑148 (MQ=255)
                          gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGaa                                                                                  <  8:13151/148‑1 (MQ=255)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                <  5:157786/149‑1 (MQ=38)
                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                <  2:294464/149‑1 (MQ=255)
                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                               <  6:356009/149‑1 (MQ=38)
                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                               <  5:231670/149‑1 (MQ=255)
                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                               <  2:303570/149‑1 (MQ=255)
                             ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                              <  8:207570/149‑1 (MQ=255)
                                gCTGTACACCGGCTAGCATAAGCGCTACATCGTTGCCCTGAACGACCGCAATTAAGCTGCAGTAGCCGAACGCAACAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTGCGCGGCCTTGCCCGGGAAGAGGCCg                                                                           <  6:81383/149‑1 (MQ=255)
                                 cTGTACACCGGCGAGCAGAAGCGCGACATCGTAGCCCTGATAGACCGCAAGAAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCATCCTTTATTATTTGTCCAGAGCCGGCCACATCCCGGCCTTGAACTCGAAGAGGCAgg                                                                          >  6:317072/1‑149 (MQ=255)
                                  tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGGAGAGGCCGGc                                                                         >  6:208342/1‑149 (MQ=255)
                                                                                                         aagaagGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGTGCCGGCCGCGTCGCGGGCGTGCCCGCGGAGAGGCCGGCCCAGCCCCCCCAAAAACACAGGTTTTATCCCCCCCCGCACGCCGCCCCTTGAGGGTTTTTAAACgcgccta  >  2:40164/1‑146 (MQ=255)
                                                                                                                                           |                                                                                                                   
TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATC  >  NC_002947_CJ‑RC/4740784‑4741038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

N/A