Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F49 I1 R1 57 62.9 2678826 98.4% 2635964 146.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 4,741,396 C→G intergenic (+526/+227) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,741,3960CG100.0% 111.4 / NA 32intergenic (+526/+227)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/18);  total (14/18)

TGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCT‑CCGCCAACTGCAATTCGTGA‑ACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTT  >  NC_002947_CJ‑RC/4741253‑4741517
                                                                                                                                                  |                                                                                                                         
tgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctc                                                                                                                       >  7:345830/1‑147 (MQ=255)
    gcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctcgacc                                                                                                                   >  8:239597/1‑143 (MQ=255)
    gcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctcgacc                                                                                                                   >  4:168140/1‑143 (MQ=255)
              aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcg                                                                                                         >  7:19869/1‑149 (MQ=255)
              aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgc                                                                                                          <  3:138378/148‑1 (MQ=255)
                  ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGACACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                                                     >  3:165605/1‑149 (MQ=255)
                   gcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                                                                    <  6:147342/149‑1 (MQ=255)
                        aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                                               >  6:277885/1‑149 (MQ=255)
                         ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                                              <  3:168140/149‑1 (MQ=255)
                            gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                           >  4:198166/1‑149 (MQ=255)
                             ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTTCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                                          <  6:259936/149‑1 (MQ=255)
                                tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                                       <  7:212715/149‑1 (MQ=255)
                                    gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTAAGCAGCTACTACGAATTGCAA‑TCGAGA‑ATCCAGGCAAAGGCCCCGGACACGTCCTCCAGCAGCGCCACGACCTTGGCCAGCGGCGCCAAGATCACGGTCACAcc                                                                                   >  2:302778/1‑149 (MQ=255)
                                       ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                                 >  8:163889/1‑148 (MQ=255)
                                            cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                           >  2:219136/1‑149 (MQ=255)
                                             tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                          >  3:72719/1‑149 (MQ=255)
                                               ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCACGGACACGTCCTCCTGCAGCGCCTCGCCCTTGGCCAGCGGCGCCTTGATAACGGTCACACCGCGATAGGCCt                                                                        >  5:195089/1‑149 (MQ=255)
                                                 tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGATTCCATGC‑ACAGGCACCGGAGACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGTTAGGCCtt                                                                       <  1:302778/148‑1 (MQ=255)
                                                 tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg                                                                      <  6:277569/149‑1 (MQ=255)
                                                   taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt                                                                     <  4:21339/148‑1 (MQ=255)
                                                    aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTa                                                                    <  8:345830/148‑1 (MQ=255)
                                                      gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                 <  7:239597/149‑1 (MQ=255)
                                                       tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACTCGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                <  6:33984/149‑1 (MQ=255)
                                                         cAGCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGa                                                              >  6:44618/1‑149 (MQ=255)
                                                           gCTCTGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCGGACACGTCCTCCT‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                           <  2:58298/149‑1 (MQ=255)
                                                            ctctGGTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                           <  8:241477/149‑1 (MQ=255)
                                                                 gTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGTCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                      >  7:51080/1‑149 (MQ=255)
                                                                 gTCACCTTCGTGCTTGAGCAGCT‑TCTGCGACTTGCATTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                      <  7:163889/149‑1 (MQ=255)
                                                                                                 ttgcaTTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGGGGGACACCTTGATCCcgtcctc                       >  8:201821/5‑149 (MQ=255)
                                                                                                   gcaTTCGTGA‑ATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg                      <  3:316388/146‑1 (MQ=255)
                                                                                                                 caggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACtt         <  4:72719/141‑1 (MQ=255)
                                                                                                                 caggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACtt         <  2:283544/141‑1 (MQ=255)
                                                                                                                      acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGt   >  5:212692/3‑149 (MQ=255)
                                                                                                                       caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGtt  <  1:2745/148‑1 (MQ=255)
                                                                                                                       caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATAACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGtt  >  1:317977/2‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                         
TGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCT‑CCGCCAACTGCAATTCGTGA‑ACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTT  >  NC_002947_CJ‑RC/4741253‑4741517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A