Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
A3 F43 I1 R1
|
57 |
79.1 |
3360134 |
98.7% |
3316452 |
147.0 |
Breseq alignment
BRESEQ :: Evidence
|
evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
RA |
NC_002947_CJ‑RC |
4,740,926 |
2 bp→CT |
intergenic (+56/+696) |
gltA → / ← yeiW |
citrate synthase/putative oxidoreductase |
|
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
* | NC_002947_CJ‑RC | 4,740,926 | 0 | T | C | 100.0%
| 26.5
/ NA
| 9 | intergenic (+56/+697) | gltA/yeiW | citrate synthase/putative oxidoreductase |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/4); total (5/4) |
* | NC_002947_CJ‑RC | 4,740,927 | 0 | G | T | 100.0%
| 23.4
/ NA
| 9 | intergenic (+57/+696) | gltA/yeiW | citrate synthase/putative oxidoreductase |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (5/4); total (5/4) |
CCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGC‑GGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAC > NC_002947_CJ‑RC/4740789‑4741048
||
cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgggccc > 7:41259/1‑145 (MQ=255)
ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCggggggc > 1:247037/1‑144 (MQ=255)
gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGC‑GGCCTTGcc < 6:25997/149‑1 (MQ=255)
gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGC‑GGCCTTGCCcgc < 7:257006/149‑1 (MQ=255)
cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGC‑GGCCTTGCCCGCGaa < 3:119270/149‑1 (MQ=255)
cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGC‑GGCCTTGCCCGCGAaga < 3:156608/149‑1 (MQ=255)
ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCGGGCGTTGCCC‑CGGCGAGGGCCGCACAGACACCCCCAAACCACAGGTTTTTTgcgccc > 2:35864/1‑149 (MQ=255)
gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTGGCGGGCCGTGCCCCCGATCCGGCCGCCAC‑GCCACCCCAAAAACCCACGTTTTTTacgccccg > 8:240240/1‑146 (MQ=255)
cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTGGC‑GGCCTTGGCCGCGAATAGGGCGGCGCAGAAACCACAAAAACTCAGTTTTTATACGCCCGCGCACGCACCCCTTTAAGGGTTTTGAACGGCGCTGCCACCACGAAc > 6:236713/1‑149 (MQ=255)
||
CCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGC‑GGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAC > NC_002947_CJ‑RC/4740789‑4741048
|
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A