Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F11 I4 R1	73	908.3	39027734	99.3%	38754539	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	4,740,819	2 bp→CT	intergenic (+56/+696)	gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,740,819	0	T	C	96.6%	1466.9 / ‑2.5	411	intergenic (+56/+697)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base T (0/14); new base C (222/175); total (222/189)
Fisher's exact test for biased strand distribution p-value = 1.44e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	4,740,820	0	G	T	99.5%	1422.2 / ‑0.9	412	intergenic (+57/+696)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base G (0/1); major base T (223/186); minor base . (0/2); total (223/189)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 412 total aligned reads displayed.

AAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC  >  AE015451_phaM1/4740689‑4740949

||

 aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                            >  2:8531471/1‑137 (MQ=255)

 aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGAAATAGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGGGGGCTGACATCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                            >  2:7907866/1‑137 (MQ=255)

     cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                        >  2:9712396/1‑137 (MQ=255)

     cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                        >  1:10045909/1‑137 (MQ=255)

      ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                       >  2:11617288/1‑137 (MQ=255)

        ccGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                     >  2:2879385/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  2:12889342/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  1:8019018/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  2:3988338/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  1:15605673/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  2:9174050/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  2:14172782/1‑137 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAAAGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTctc                                                                                                                   >  2:5739031/1‑135 (MQ=255)

          gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGAAATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                   >  2:16068783/1‑137 (MQ=255)

          gTCCGCGCCAACTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTtgc                                                                                                                   >  2:8216750/1‑137 (MQ=255)

            ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                 <  1:6601023/137‑1 (MQ=255)

            ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                 <  1:3848265/137‑1 (MQ=255)

              gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                               <  2:366296/137‑1 (MQ=255)

                      tggacacCGGCGAGCAAAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAACCAAACGCAAGAAGGCTACCCTCGGGAAGCC‑TTTTTTGTTTGTCCTGTGCCGGCCTCTTCGTGGCTTTGCCcga                                                                                                       <  2:1673285/134‑2 (MQ=255)

                      tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                       <  2:14403239/137‑1 (MQ=255)

                          cacCGGCGAGCAGAATCGCGACATCGTTACCCTGAAAGACCGCAAGGAAGCTGCAGTAGCAGAACGAAAGAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGTCCTCTTCGCGGCCTTGCCCGCGAag                                                                                                   <  2:8074125/137‑1 (MQ=255)

                          cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTGTTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag                                                                                                   <  2:19429357/137‑1 (MQ=255)

                          cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag                                                                                                   <  2:8979523/137‑1 (MQ=255)

                            ccGGCGAGCAGAAGCGCGACATCGTTGCCCGGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                 <  2:11008723/137‑1 (MQ=255)

                             cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACAGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                                                >  1:19344322/1‑137 (MQ=255)

                             cGGCGAGCAGAAGCGCGAAATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCACGAAGAgg                                                                                                >  1:18403495/1‑137 (MQ=255)

                               gCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCTTTGGGCGGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                              <  1:5141022/137‑1 (MQ=255)

                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGTCTTGTCCGCGAAGAGGCCGGc                                                                                           >  1:16563674/1‑137 (MQ=255)

                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgga                                                                                           >  1:11872550/1‑136 (MQ=39)

                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:16425430/1‑137 (MQ=255)

                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:17431662/1‑137 (MQ=255)

                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTCGCGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  1:4055104/1‑137 (MQ=255)

                                  aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCAGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCACGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           >  2:17892308/1‑137 (MQ=255)

                                  aGCAGAAGCGCGACATCGTTGCCCGGAAAGACCCCAAGTAAGCTGCAGTAGCCGAACACAAGAAGGCTGCTCTCGGGCAGTC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGTGGCCTTGCCCGCGAAGAGGCCGGc                                                                                           <  1:13620041/137‑1 (MQ=255)

                                   gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCCCGAAGAGGACGGca                                                                                          >  1:5922726/1‑137 (MQ=255)

                                   gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCCTGCCCGCGAAGAGGCCGGca                                                                                          >  1:4667324/1‑137 (MQ=255)

                                   gCAGAAGCGCGACATCGCTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCATCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca                                                                                          >  2:4514620/1‑137 (MQ=38)

                                    cAGAAGCTCAACATCTTTGCCCTGAAAGACCGCAGGGAAGCTGAAGTAGCAAAACACAAGAAGGCTACCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcac                                                                                         <  1:16505578/137‑1 (MQ=255)

                                    cAGAAGCGCGACATCTTTGCCCTGAAAGACCGCAAGTAAGCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcac                                                                                         <  1:4396220/137‑1 (MQ=255)

                                    cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAGGGCTGCCCTTGGGCAG‑CTTTTTTTATTTGTCCTGTTCCGTCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcac                                                                                         <  1:1580569/137‑1 (MQ=255)

                                     aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTACGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAAGAGACAGGccca                                                                                        >  1:13096894/1‑137 (MQ=255)

                                     aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcaca                                                                                        >  1:3558869/1‑137 (MQ=255)

                                     aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcaca                                                                                        >  2:3200191/1‑137 (MQ=255)

                                          cgcgACATTGTTGCCCGGAAGGACCGAAGGGAAGCTGAGGGAGCAGAACAAAAGAAGGTTGCCTTTGGGAAG‑CTTTTTTTGTTTGTTTTTTGCCGTCCTTTTTGCGGCTTTTCCCGGGAAGAGGCCGGCACAGacac                                                                                   <  1:10641105/137‑1 (MQ=255)

                                            cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGTCTTTCCCGTGAAGAGGCCGGCACAGAcaccc                                                                                 >  1:19177435/1‑136 (MQ=255)

                                            agACATTGTTGCCCGGAAAGACCGAAAGGAATCTGCAGTACCCGAACGAAAGAAGGCTGCCCTCGGGCAG‑CTTTTTTTGTTTTTCTTGTGCCGGCCTCTTTGTGGCTTTGCCCGCGAGGGGGCCGGCACAGAcacca                                                                                 <  1:7547552/136‑1 (MQ=255)

                                               cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAAAGGCCGGCACAGACACCACaa                                                                              >  1:2746723/1‑137 (MQ=38)

                                                 tCGTTTCCCTGAAAGACCGCAGGGAAGCTGCAGTAGTCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTTTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                            <  1:12702647/137‑1 (MQ=38)

                                                     tGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTc                                                                        <  2:7255601/137‑1 (MQ=255)

                                                     tGCCCGGAAAGACCGCAAGGAATCTGCAGGAGCCGAACGAAAGAAGGCTGCCCTTGGGAAG‑CTTTTTTTGTTTGTCTTGTACCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTc                                                                        <  1:200192/137‑1 (MQ=37)

                                                       cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGACCTGCCCGCGAAAAGGCCGGCAAAGAAAACACAAAACCCCaa                                                                      >  1:17404399/1‑136 (MQ=255)

                                                              aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTTGGGCAGCC‑TTTTTTGTTTGTCCTGTTCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                               <  1:8531471/137‑1 (MQ=255)

                                                              aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct                                                               <  2:16586523/137‑1 (MQ=255)

                                                               gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                              >  2:5281023/1‑137 (MQ=39)

                                                               gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                              >  2:6891245/1‑137 (MQ=255)

                                                               gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACAACACAAAAAATCAGTTTTtctc                                                              >  2:1855480/1‑137 (MQ=38)

                                                               gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGGCGGCACAGACACCACAAAACCTCCGTTTTtctc                                                              >  1:9995844/1‑137 (MQ=255)

                                                               gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAAAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                              >  2:14647947/1‑137 (MQ=38)

                                                                aCCGCAAGGAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCTTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGTCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                             <  1:12049609/137‑1 (MQ=255)

                                                                  cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGCCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                           <  1:18778949/137‑1 (MQ=255)

                                                                    cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCCTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTccgccc                                                         >  1:10412546/1‑137 (MQ=255)

                                                                     aaGTAAGCTGCAGTAGCCGAACGCACGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCTTGGCCCTGCCCGGAAAGAGGCCTGCACAAAAACCACCAAATCCCAGTTTTTCTccaccca                                                        >  2:2352523/1‑136 (MQ=255)

                                                                     aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAAACACCACAAAACCTCAGTTTTTCTccgcccg                                                        >  2:13366322/1‑137 (MQ=255)

                                                                     aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAACCTCCGTTTTTCTccgcccg                                                        >  2:6208153/1‑137 (MQ=38)

                                                                     aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGACTTTCCCGCAAAAAGGACAACACCAAAAACACAAAAAATCCACTTTTCTcccccca                                                        >  1:4010090/1‑136 (MQ=255)

                                                                       gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTTTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                      <  2:9438649/137‑1 (MQ=255)

                                                                       gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGAACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                      >  2:1508832/1‑137 (MQ=39)

                                                                       gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                      <  2:8827825/137‑1 (MQ=255)

                                                                          aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                   <  2:12539525/137‑1 (MQ=255)

                                                                           gCTGCAGTAGCCGAACGCAAGAAGGCTGCCTTCGGGCAGCC‑TTTTTTATTTGTCCTTTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                  <  2:6508054/137‑1 (MQ=255)

                                                                                 ttAGCCGAACGCAAGAGGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGTGGCCTTGCCCGTGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                            <  1:11136692/136‑1 (MQ=255)

                                                                                  tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCTCGGGCTTGCCCCCGAAGAGGACAGCACAAACAACAAAAAACCCCCATTTTTCTCCACCCACTCCCGCAAcccc                                           >  1:2383175/1‑136 (MQ=255)

                                                                                  tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCt                                           <  2:7177103/137‑1 (MQ=255)

                                                                                  tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCACTCACACAACCCt                                           >  2:15412002/1‑137 (MQ=255)

                                                                                   aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  1:19455659/137‑1 (MQ=255)

                                                                                   aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  2:10648025/137‑1 (MQ=39)

                                                                                   aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  2:1308542/137‑1 (MQ=255)

                                                                                   aGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  2:1481761/137‑1 (MQ=255)

                                                                                   aGCCGAACGAAAGAAGGCTGCCCTTGGGCAG‑CTTTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  1:3325881/137‑1 (MQ=255)

                                                                                   aGCCGAACACAAGAAGTCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGTGGCTTTTCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                          <  1:17790158/137‑1 (MQ=255)

                                                                                     ccGAACGCAAGAAGGCTGCCCTCTGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGTCCTTTc                                        <  2:18814429/137‑1 (MQ=38)

                                                                                       gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCACGAAGAGGCCAGCACAGACACCCCAAAACCTCAGTTTTTCTCCACCCGCTCACACAGCCCTTTcca                                      >  1:15215663/1‑135 (MQ=255)

                                                                                            cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                 >  2:13700426/1‑137 (MQ=255)

                                                                                            aaagaagGCTTCCCTCGGGCA‑TCTTTTTTTGTTTTTCCTGTGCTGTCCTTTTTGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                 <  1:8247797/136‑1 (MQ=255)

                                                                                                  ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCAGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAAGCAGAACTTTCAGGGTGTTGAACg                           >  1:14128270/1‑137 (MQ=255)

                                                                                                  ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCCTGCCCACGAAGAGGCCGGCACAAACACCACAAAAACCCAGTTTTTCTCCGCCCGCTCACGCAACCCCTTCAGGGTATTGAACg                           >  1:14567275/1‑137 (MQ=255)

                                                                                                         cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCCTTCAGGGTATTGAACGGCGCATc                    >  1:14325182/1‑137 (MQ=39)

                                                                                                          tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCCCGCAGAGGACGGCACCGACACCACAAAACCCCAACTTTTCTCCCCCCTCTCACGCAGCCCTTTCCGGGTATTGAAAAgcgccccc                   >  1:7197583/1‑133 (MQ=255)

                                                                                                          tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCTCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCCGCCCACTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                   >  1:13285759/1‑137 (MQ=255)

                                                                                                          tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                   >  2:10506271/1‑137 (MQ=255)

                                                                                                          tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCACGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCCCAGTTTTTCTCCGCCCGCTCACACAGCCCCTTCAGGGTATTGAACGGCGCATcc                   >  1:7096742/1‑137 (MQ=255)

                                                                                                             ggCAGCC‑TTTTTTGTTTGTCTTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                <  1:4884909/137‑1 (MQ=255)

                                                                                                              gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca               <  2:12250558/137‑1 (MQ=255)

                                                                                                               cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCTCGAAGAGGACGGCACAAACACCACAAAAACCCAATTTTTCTCCACCCCCCCCCGCAACCCTTTCAGGGTATTGAACGGCGCACCcaccac              >  1:8846209/1‑137 (MQ=255)

                                                                                                               cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAAACACCACAAAACCCCCGTTTTTCTCCTCCCGCTCCCGCAACCCCTTCAGGGTATTGAACGGTGTATCcaccac              >  1:5393906/1‑137 (MQ=25)

                                                                                                                    cttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc          <  1:12462533/136‑1 (MQ=255)

                                                                                                                     ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGCTTTTCTCCTCCCGCTCACGCAGCCATTTCAGGGTATTTAACAGCGCATCCACCACTAACt         >  2:12021401/1‑137 (MQ=38)

                                                                                                                     ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCACGAAGAGGCCGGCACAAACACCACAAAAACCCAGTTTTTCTCCGCCCGCTCACGCAGCCCCTTCAGGGTCTTGAACAGCGCATCCACCACGAACt         >  1:18063317/1‑137 (MQ=255)

                                                                                                                      tttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGGAGAAGCCGGCACAGAAACCACAAAACCTCAGTTTTTCTCCGCCCGTTCACGCAGCCCTTTCAGGGTATTGCACGGTTCACCCCCCACGAACtt        >  2:11080812/1‑137 (MQ=255)

                                                                                                                         ttATTTGTCCTGTGCCGTCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt     <  1:13444811/137‑1 (MQ=255)

||

AAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC  >  AE015451_phaM1/4740689‑4740949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A