Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F11 I4 R1 73 908.3 39027734 99.3% 38754539 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,701,209 C→A intergenic (+1955/‑2050) PP_5002 CDS (conserved protein of unknown function) → / → PP_5009 CDS (conserved protein of unknown function) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,701,2090CA100.0% 2677.2 / NA 738intergenic (+1955/‑2050)PP_5002 CDS (conserved protein of unknown function)/PP_5009 CDS (conserved protein of unknown function)–/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (387/351);  total (387/351)

Only 100 of 752 total aligned reads displayed.

TCGCCGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGCGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAGCCGATCGC  >  AE015451_phaM1/5701076‑5701342
                                                                                                                                     |                                                                                                                                      
 cGCCGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGgagcgc                                                                                                                                    <  1:4265173/137‑1 (MQ=255)
 cGCCGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGgagcgc                                                                                                                                    <  2:12030479/137‑1 (MQ=255)
    cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCTTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCa                                                                                                                                 <  2:18131996/137‑1 (MQ=255)
    cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCa                                                                                                                                 >  1:6940062/1‑137 (MQ=255)
      tGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                               <  2:3630655/137‑1 (MQ=255)
       gCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACg                                                                                                                              <  1:5099362/137‑1 (MQ=255)
       gCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACg                                                                                                                              <  1:14585425/137‑1 (MQ=255)
        ctctCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGa                                                                                                                             <  2:12597676/137‑1 (MQ=255)
         tctcGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAg                                                                                                                            <  1:10658085/137‑1 (MQ=255)
             ggCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGa                                                                                                                        >  2:14705994/1‑137 (MQ=255)
              gCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGAt                                                                                                                       <  1:12594303/137‑1 (MQ=255)
              gCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGAt                                                                                                                       <  1:15034471/137‑1 (MQ=255)
                cTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCt                                                                                                                     >  2:17543326/1‑137 (MQ=255)
                   tGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                                  <  2:14299548/137‑1 (MQ=255)
                   tGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                                  <  1:6243932/137‑1 (MQ=255)
                   tGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCAGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                                  <  1:18696015/137‑1 (MQ=255)
                   tGCCGGTCTGCGCTCGGCGGCCCTCAACGCCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                                  <  1:5314441/137‑1 (MQ=255)
                     ccGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACgag                                                                                                                >  1:15631079/1‑137 (MQ=255)
                     ccGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACgag                                                                                                                >  1:11899581/1‑137 (MQ=255)
                      cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACgaga                                                                                                               >  2:10610445/1‑137 (MQ=255)
                      cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACgaga                                                                                                               >  1:17946097/1‑137 (MQ=255)
                      cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACgaga                                                                                                               >  2:9533308/1‑137 (MQ=255)
                       ggTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGAc                                                                                                              <  2:14509281/137‑1 (MQ=255)
                        gTCTGCGCTCTGCGGCCCCCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTGCGAGACg                                                                                                             <  2:12262862/137‑1 (MQ=255)
                        gTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                             <  1:11017095/137‑1 (MQ=255)
                        gTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                             <  2:15564570/137‑1 (MQ=255)
                        gTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                             <  2:7654979/137‑1 (MQ=255)
                        gTCTGCGCTAGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                             <  2:10396039/137‑1 (MQ=255)
                           tGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTg                                                                                                          >  1:14061224/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGATCGGATCTACGAGACGGTGa                                                                                                         >  1:9692936/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         <  1:3318472/137‑1 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  2:6629425/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  1:1684516/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  1:16297855/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         <  2:13332946/137‑1 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  1:2518897/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  2:17830293/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  2:8724711/1‑137 (MQ=255)
                            gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                         >  1:7687629/1‑137 (MQ=255)
                             cgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGaa                                                                                                        >  2:12961310/1‑137 (MQ=255)
                              gcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                       >  2:527500/1‑137 (MQ=255)
                              gcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                       <  2:5061033/137‑1 (MQ=255)
                                tCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTc                                                                                                     >  1:10263753/1‑137 (MQ=255)
                                tCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTc                                                                                                     >  2:11631428/1‑137 (MQ=255)
                                tCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTc                                                                                                     >  1:15338770/1‑137 (MQ=255)
                                tCGGCGGCCCTCAACGCCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTc                                                                                                     >  1:17768375/1‑137 (MQ=255)
                                 cggcggCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGATGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTcc                                                                                                    <  1:1141146/137‑1 (MQ=255)
                                     ggcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAgg                                                                                                >  2:1524691/1‑137 (MQ=255)
                                      gcCCTCAACTTCTACGTCAACACCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGt                                                                                               <  2:3984563/137‑1 (MQ=255)
                                      gcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGt                                                                                               <  1:3707241/137‑1 (MQ=255)
                                      gcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGt                                                                                               <  2:9150320/137‑1 (MQ=255)
                                       cccTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTa                                                                                              <  1:8761326/137‑1 (MQ=255)
                                       cccTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTa                                                                                              <  1:15951480/137‑1 (MQ=255)
                                         cTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTaaa                                                                                            >  2:3805217/1‑137 (MQ=255)
                                         cTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTaaa                                                                                            <  2:15779457/137‑1 (MQ=255)
                                          tCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAAc                                                                                           >  1:2819451/1‑137 (MQ=255)
                                          tCAACGTCTACGTCAAAGCCAAGGACCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGGAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAGAc                                                                                           >  2:16641284/1‑137 (MQ=255)
                                             aCGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGa                                                                                        >  2:3233308/1‑137 (MQ=255)
                                                tCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCt                                                                                    <  1:19503980/137‑1 (MQ=255)
                                                    agTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGACGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAggg                                                                                >  2:17248266/2‑137 (MQ=255)
                                                     gTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGa                                                                               >  2:19162223/1‑137 (MQ=255)
                                                       cAACGCCAAGGGCCTCGACGACCGCGCCTTCGACGAGGAGCGGCTGAAGGAGCTGGAAGGCGTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGCCGGTGAAGTCCCAGGTAGACTGC‑CCTAGGGCtt                                                                             >  2:13853353/1‑137 (MQ=255)
                                                         aCGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGAtttt                                                                           >  2:1626160/1‑137 (MQ=255)
                                                         aCGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGAtttt                                                                           <  1:13147466/137‑1 (MQ=255)
                                                            ccAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAAt                                                                        >  1:4628408/1‑137 (MQ=255)
                                                             cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAAtt                                                                       >  1:17318973/1‑137 (MQ=255)
                                                             cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAAtt                                                                       >  1:8502874/1‑137 (MQ=255)
                                                             cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAAtt                                                                       >  2:19332304/1‑137 (MQ=255)
                                                             cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑ACTAGGGATTTTAAtt                                                                       >  2:10103279/1‑137 (MQ=255)
                                                             cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGAAGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAAtt                                                                       >  1:17457922/1‑137 (MQ=255)
                                                              aaGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCCTAGGGATTTTAAtt                                                                       <  2:11337542/137‑1 (MQ=255)
                                                                gggCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCAt                                                                    <  1:5785329/137‑1 (MQ=255)
                                                                  gCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAAGTAAACTGA‑CCTAGGGATTTTAATTCATTa                                                                  >  2:1093968/1‑137 (MQ=255)
                                                                   ccTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAg                                                                 <  2:586540/137‑1 (MQ=255)
                                                                   ccTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAg                                                                 <  1:9965049/137‑1 (MQ=255)
                                                                     tCGACGACCGCGCCTGCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCa                                                               <  2:18984778/137‑1 (MQ=255)
                                                                       gacgacCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAgg                                                             >  1:12179735/1‑137 (MQ=255)
                                                                        acgacCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGa                                                            >  1:16622769/1‑137 (MQ=255)
                                                                         cgacCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAg                                                           >  1:9024693/1‑137 (MQ=255)
                                                                             cgcgCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTGTAATTCATTAGCAGGAGTCtt                                                       <  2:2392688/137‑1 (MQ=255)
                                                                              gcgcCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCttt                                                      <  2:8466406/137‑1 (MQ=255)
                                                                              gcgcCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCttt                                                      <  2:12536515/137‑1 (MQ=255)
                                                                               cgcCTTCGCCGAGGAGCGGCTGAAGAAGCTGGAAGGACTACTGGCCGAGGCGAGAGCGCTCAACGAGCGAATCTACGAGACGGTAAAGTCCAAGGTAAAACGA‑CATAGGGATTTCAATTCATTAGCAGGAGTCTTTg                                                     >  2:7777737/1‑137 (MQ=255)
                                                                                gcCTTCGCCGAGGAGTGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGt                                                    <  2:7016740/137‑1 (MQ=255)
                                                                                  cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTAt                                                  <  1:10046029/137‑1 (MQ=255)
                                                                                    tCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGTTGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGGGAAGGCCAAGGTAAACTGA‑CCTAGGGATGTTAATTCATTAGCAGGAGTCGGTGTATgt                                                <  2:8316431/137‑2 (MQ=255)
                                                                                      gCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGccc                                              <  2:7408628/137‑1 (MQ=255)
                                                                                       ccGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCt                                             >  2:2050337/1‑137 (MQ=255)
                                                                                             agCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGata                                       >  1:3115534/1‑137 (MQ=255)
                                                                                                  cTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCgag                                  >  1:3981238/1‑137 (MQ=255)
                                                                                                             ggAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCcgcgc                       <  2:4499186/137‑1 (MQ=255)
                                                                                                             ggAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCcgcgc                       <  2:7181439/137‑1 (MQ=255)
                                                                                                             ggAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCcgcgc                       <  1:19162223/137‑1 (MQ=255)
                                                                                                             ggAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCcgcgc                       <  1:11734128/137‑1 (MQ=255)
                                                                                                             ggAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGGGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCcgcgc                       <  2:2973782/137‑1 (MQ=255)
                                                                                                               aaGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCgcgg                     >  2:18317837/1‑137 (MQ=255)
                                                                                                                      gctgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCt              >  1:7263876/1‑137 (MQ=255)
                                                                                                                        tgGCCGAGGCGGGAGCGCTCAAAGAGCGGATCTAAGAGACGGTGAAGTCCAAGGTAAACTGA‑CCCAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGGTCGCCTGCGCGGCGACCCTGa            >  1:13593506/1‑137 (MQ=255)
                                                                                                                         ggCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                                <  1:7875449/100‑1 (MQ=255)
                                                                                                                         ggCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGaa           >  2:8083481/1‑137 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCGCCGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGCGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGA‑CCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAGCCGATCGC  >  AE015451_phaM1/5701076‑5701342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A