Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F42 I4 R1 76 794.2 35354760 99.3% 35107276 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 4,586,030 2 bp→TC intergenic (+113/+101) PP_4061 CDS (conserved protein of unknown function) → / ← PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,586,0300CT100.0% 658.1 / NA 184intergenic (+113/+102)PP_4061 CDS (conserved protein of unknown function)/PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)–/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/178);  total (6/178)
*AE015451_phaM14,586,0310TC99.5% 716.3 / ‑6.1 184intergenic (+114/+101)PP_4061 CDS (conserved protein of unknown function)/PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)–/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (5/178);  total (6/178)
Fisher's exact test for biased strand distribution p-value = 3.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 184 total aligned reads displayed.

ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GG‑TAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAG  >  AE015451_phaM1/4585902‑4586111
                                                                                                                                ||                                                                                    
aCCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTTAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggta                                                                              <  2:17369401/137‑4 (MQ=255)
    gTGAAGGCTTAAGGGTAGGCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCAGG‑TAAAcc                                                                          <  2:9107788/137‑1 (MQ=255)
      gAAGGCTTAAGGTTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:7701920/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTTAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:4074808/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:16193634/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:461611/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:12468865/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:12341947/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:9444435/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:9326002/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:7920382/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:1932856/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:1932895/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:2175279/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:5313669/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:344773/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:344810/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:5219516/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:4074766/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:13351946/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:13443449/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:14913905/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  1:14825871/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:16406526/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:1707050/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:2336633/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:2336663/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:9005061/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:4938643/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:5240592/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:5735064/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:6276718/137‑1 (MQ=255)
      gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGACGACCGGATGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCg                                                                        <  2:13721810/137‑1 (MQ=255)
        aGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCt                                                                      <  1:16690372/137‑1 (MQ=255)
         ggCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTc                                                                     <  2:4851699/137‑1 (MQ=255)
           cTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCt                                                                   <  2:6311283/137‑1 (MQ=255)
           cTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCt                                                                   <  1:10226798/137‑1 (MQ=255)
           cTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCt                                                                   <  1:10226756/137‑1 (MQ=255)
                gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAg                                                              <  2:7305406/137‑1 (MQ=39)
                gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAg                                                              <  1:3544542/137‑1 (MQ=39)
                gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAg                                                              <  1:14342860/137‑1 (MQ=39)
                gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAg                                                              <  1:17320987/137‑1 (MQ=39)
                 ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAgg                                                             <  1:13720593/137‑1 (MQ=39)
                  gTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAggg                                                            >  2:15980823/1‑137 (MQ=39)
                   tAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑a                                                          <  1:5615701/137‑1 (MQ=39)
                     gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑A‑cc                                                       <  1:5408656/137‑2 (MQ=39)
                     gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑A‑cc                                                       <  2:1222766/137‑2 (MQ=39)
                     gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑A‑cc                                                       <  1:6993812/137‑2 (MQ=39)
                     gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATGGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑A‑cc                                                       <  1:11622341/137‑2 (MQ=38)
                      aCCACGGCACCTGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGG‑A‑ccg                                                      <  1:17612919/137‑3 (MQ=38)
                      aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑A‑ccg                                                      <  1:4298101/137‑3 (MQ=38)
                      aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑A‑ccg                                                      <  1:17612920/137‑3 (MQ=38)
                            gCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCgg                                                 <  2:16691234/137‑1 (MQ=38)
                                  gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGG‑ACCGTGCCGGCCTTGa                                           <  1:9129932/137‑1 (MQ=38)
                                  gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGa                                           <  1:104403/137‑1 (MQ=38)
                                  gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGa                                           <  1:1183988/137‑1 (MQ=38)
                                  gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGa                                           <  2:1269767/137‑1 (MQ=38)
                                   cGTGAACAGCTGATTTGCGCTGCTCCCTTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTTGCGGG‑TAAAACCGTCTCTCCCAAGG‑ACCATGCCGGGCTTgga                                          >  2:13271509/1‑135 (MQ=255)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGG‑ACCGTGCCGGCCTTGAATgcg                                      <  2:35579/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:10646698/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:16896849/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:4578707/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  2:2365762/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:1326047/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:14356160/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  2:14398359/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:10763293/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  2:6163328/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:6396178/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  2:11352480/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  2:10830778/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:17346293/137‑1 (MQ=38)
                                       aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                      <  1:8276060/137‑1 (MQ=38)
                                        aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcgg                                     <  1:11280243/137‑1 (MQ=38)
                                        aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcgg                                     <  1:282385/137‑1 (MQ=38)
                                         cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggc                                    <  2:4127138/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCATGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  1:4801406/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  2:4408124/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  2:8619136/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  1:5629539/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  2:2797895/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  1:5887575/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  1:7108199/137‑1 (MQ=38)
                                          aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcggcg                                   <  1:15980823/137‑1 (MQ=38)
                                            cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCt                                 <  1:7604799/137‑1 (MQ=38)
                                             tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTg                                <  2:2534968/137‑1 (MQ=38)
                                                 cTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGcccc                            <  2:3947011/137‑1 (MQ=38)
                                                 cTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGcccc                            <  1:10447191/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGGTGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  1:1717479/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  1:11740570/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  1:8079579/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  2:6103187/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  2:13092572/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  2:10266975/137‑1 (MQ=38)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑GGATAAACCCGCTCCTACCAGGG‑ACCGTGCCTGCCTTTAATGCGGCGCTGCCCCTGTTa                       <  2:4525905/137‑1 (MQ=37)
                                                      ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGGGAAGATCTTGCATGGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCGACCATGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                       <  1:14450540/137‑1 (MQ=38)
                                                       tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                      <  1:5547242/137‑1 (MQ=38)
                                                       tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                      <  1:15597573/137‑1 (MQ=38)
                                                                 aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCt                                 >  2:5330625/1‑116 (MQ=37)
                                                                           gATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGG‑TGAAACCACTCCCCCCAGGGAA‑CGTTCCGGCCCTTAATGCGGAGCTTCCCCTGGTAGGAGCGGGTTTccccccaaaa  >  2:14078240/1‑131 (MQ=255)
                                                                                                                                ||                                                                                    
ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GG‑TAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAG  >  AE015451_phaM1/4585902‑4586111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCG  >  AE015451_phaM1/4585893‑4586117
                                                                                                                                         |                                                                                          
CCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCC                                                                               <  LH00941:114:22FVMWLT1:2:2203:17909:2361/151‑1 (MQ=60)
CCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCC                                                                               <  LH00941:114:22FVMWLT1:2:2130:33542:11376/151‑1 (MQ=60)
    GAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTC                                                                           <  LH00941:114:22FVMWLT1:2:2213:14525:1464/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGTTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:2144:19425:10624/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGNAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:2261:32599:3690/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:2253:35456:11200/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:2144:19435:10640/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:2207:15136:5596/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:2137:39145:25820/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                      <  LH00941:114:22FVMWLT1:2:1146:21394:21977/151‑1 (MQ=60)
          CCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA                                                                     <  LH00941:114:22FVMWLT1:2:2157:3126:7661/151‑1 (MQ=60)
            GGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTCCCAGG                                                                   >  LH00941:114:22FVMWLT1:2:1238:49314:4395/1‑151 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:2229:12510:2841/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:1147:40069:7469/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:1153:30177:11568/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:2224:33894:21336/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:1232:43730:8238/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:2155:15256:14291/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:1215:48695:3450/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:2118:29391:10816/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:2208:46430:8718/151‑1 (MQ=60)
             GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTCGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                  <  LH00941:114:22FVMWLT1:2:2226:16273:27389/151‑1 (MQ=60)
               GAGGGCTTAAGGGTAGAACACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTTCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGGAGATCTTGCATTGATCGGGCCGGCCTCTGCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2123:40476:20151/151‑1 (MQ=60)
               GAAGGCTTAAGGTTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1253:5576:11328/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTTAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1165:6039:26412/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1132:46735:7645/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1167:24159:13922/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2228:21126:1977/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2248:21635:5484/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2108:4199:26653/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2163:8156:18038/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2228:21145:1977/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2128:27274:12241/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2123:40485:20135/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1241:33626:23306/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2226:32720:19559/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1255:45237:25580/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1256:27099:22793/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2110:8590:24747/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1261:41955:3978/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1158:11179:9375/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:2270:12871:12113/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1123:46985:23386/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1132:46735:7613/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1132:46744:7629/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1132:46754:7613/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1136:13851:3434/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1147:17623:29535/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1156:1435:22281/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1165:6020:26412/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1165:6029:26396/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1168:42279:29423/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1108:7804:17237/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1215:27820:21929/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1106:20840:19767/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1106:20831:19783/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1214:37851:7213/151‑1 (MQ=60)
               GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                               <  LH00941:114:22FVMWLT1:2:1215:27811:21913/151‑1 (MQ=60)
                 AGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑Ccg                                                             <  LH00941:114:22FVMWLT1:2:2255:17345:26332/151‑3 (MQ=60)
                 AGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑Ccg                                                             <  LH00941:114:22FVMWLT1:2:2255:17364:26332/151‑3 (MQ=60)
                    CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGC                                                          <  LH00941:114:22FVMWLT1:2:2123:14451:15908/151‑1 (MQ=60)
                    CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGC                                                          <  LH00941:114:22FVMWLT1:2:2123:14442:15924/151‑1 (MQ=60)
                    CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGC                                                          <  LH00941:114:22FVMWLT1:2:1136:28994:19383/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:1206:31398:28030/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:2139:1425:17205/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:2218:6492:28766/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:1118:40217:13106/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:2234:10337:12657/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:1157:44673:19959/151‑1 (MQ=60)
                         GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCC                                                     <  LH00941:114:22FVMWLT1:2:2265:23937:16933/151‑1 (MQ=60)
                          GGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCT                                                    <  LH00941:114:22FVMWLT1:2:2113:37009:21224/151‑1 (MQ=60)
                          GGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCT                                                    <  LH00941:114:22FVMWLT1:2:2209:19194:1769/151‑1 (MQ=60)
                            TAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTG                                                  <  LH00941:114:22FVMWLT1:2:1220:37675:16196/151‑1 (MQ=60)
                              GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAA                                                <  LH00941:114:22FVMWLT1:2:1242:38091:9903/151‑1 (MQ=60)
                              GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAA                                                <  LH00941:114:22FVMWLT1:2:1217:4439:8046/151‑1 (MQ=60)
                              GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAA                                                <  LH00941:114:22FVMWLT1:2:1217:4430:8030/151‑1 (MQ=60)
                              GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAA                                                <  LH00941:114:22FVMWLT1:2:2127:27755:1416/151‑1 (MQ=60)
                              GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATGGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAA                                                <  LH00941:114:22FVMWLT1:2:2145:35724:27998/151‑1 (MQ=60)
                               ACCACGGCACCTGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAAT                                               <  LH00941:114:22FVMWLT1:2:2270:5586:1576/151‑1 (MQ=60)
                               ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAAT                                               <  LH00941:114:22FVMWLT1:2:2208:30713:19799/151‑1 (MQ=60)
                               ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAAT                                               <  LH00941:114:22FVMWLT1:2:2270:5604:1576/151‑1 (MQ=60)
                               ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAAT                                               <  LH00941:114:22FVMWLT1:2:2102:24566:16869/151‑1 (MQ=60)
                               ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAAT                                               <  LH00941:114:22FVMWLT1:2:1169:25619:11873/151‑1 (MQ=60)
                                 CACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGC                                             <  LH00941:114:22FVMWLT1:2:1257:12519:26300/151‑1 (MQ=60)
                                           GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCC                                   <  LH00941:114:22FVMWLT1:2:2105:7388:20584/151‑1 (MQ=60)
                                           GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCC                                   <  LH00941:114:22FVMWLT1:2:1102:12242:21721/151‑1 (MQ=60)
                                           GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCC                                   <  LH00941:114:22FVMWLT1:2:1120:12852:8334/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2130:24806:9375/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2259:15940:2761/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2266:44618:5580/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2265:31268:28030/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2265:31259:28046/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2219:36843:6172/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2259:15949:2777/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1262:23465:7437/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1203:7980:26637/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2149:3265:2906/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1227:19287:24539/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1232:23216:26829/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1169:25823:26156/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1126:46097:13746/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1122:24251:16484/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2132:42695:5516/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2146:13564:11520/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGACCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:2108:24686:19927/151‑1 (MQ=60)
                                                AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCAGTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                              <  LH00941:114:22FVMWLT1:2:1209:44072:16036/151‑1 (MQ=60)
                                                 ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                             <  LH00941:114:22FVMWLT1:2:2140:41216:15123/151‑1 (MQ=60)
                                                 ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                             <  LH00941:114:22FVMWLT1:2:1105:10245:19223/151‑1 (MQ=60)
                                                  CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAG                            <  LH00941:114:22FVMWLT1:2:1221:32294:19879/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTTCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑CTGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:2144:19074:27565/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1207:23530:15203/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1224:2322:25163/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1220:7693:22521/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1220:7712:22521/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:2244:5909:25323/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1244:16402:3978/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1244:16411:3962/151‑1 (MQ=60)
                                                   AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                           <  LH00941:114:22FVMWLT1:2:1244:16421:3978/151‑1 (MQ=60)
                                                     gagacagGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                         <  LH00941:114:22FVMWLT1:2:1215:32572:29711/144‑1 (MQ=60)
                                                     CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                         <  LH00941:114:22FVMWLT1:2:2214:30390:5307/151‑1 (MQ=60)
                                                     CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                         <  LH00941:114:22FVMWLT1:2:1251:28430:25388/151‑1 (MQ=60)
                                                          CTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGT                    <  LH00941:114:22FVMWLT1:2:2127:39450:3258/151‑1 (MQ=60)
                                                          CTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGT                    <  LH00941:114:22FVMWLT1:2:1127:14664:14066/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGGTGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:1128:10051:23850/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:2147:18205:24203/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:1128:10060:23866/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:1259:5789:6893/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:2220:46347:20488/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:2223:48159:24106/151‑1 (MQ=60)
                                                               CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGGGAAGATCTTGCATGGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCGACCATGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC               <  LH00941:114:22FVMWLT1:2:2220:46365:20488/151‑1 (MQ=60)
                                                                TGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC              <  LH00941:114:22FVMWLT1:2:1219:12371:13554/151‑1 (MQ=60)
                                                                TGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC              <  LH00941:114:22FVMWLT1:2:2238:25250:24907/151‑1 (MQ=60)
                                                                            AGTTTAAGGATGACCGGGTGCTTGGCAAGGGGAAGATCTTGCATTGATTGGGCCGGCCTCTTCGCGGGTAAACCCACTCCTACCAGGGACC‑GTACCGGCCTTGAATGCGGCGCTGCCCCTGGGAGGAGCGGGTTTACCCGCtcctaccagg  <  LH00941:114:22FVMWLT1:2:2262:48769:21416/151‑11 (MQ=60)
                                                                                                                                         |                                                                                          
CCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCG  >  AE015451_phaM1/4585893‑4586117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑