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Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F11 I4 R1	74	758.2	33768536	99.3%	33532156	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451_phaM1	4,372,651	Δ54,766 bp		PP_3849 CDS (putative calcium‑binding protein_ hemolysin‑type)–[PP_3920 CDS (putative Phage integrase)]	78 genes PP_3849 CDS (putative calcium‑binding protein_ hemolysin‑type), PP_3850 CDS (conserved protein of unknown function), PP_5637 CDS (conserved protein of unknown function), PP_3851 CDS (conserved protein of unknown function), PP_3852 CDS (conserved protein of unknown function with BNR domain), PP_5638 CDS (conserved protein of unknown function), PP_3854 CDS (Lysozyme), PP_5639 CDS (conserved exported protein of unknown function), PP_3855 CDS (conserved protein of unknown function), PP_3856 CDS (conserved protein of unknown function), PP_3857 CDS (conserved protein of unknown function), PP_3858 CDS (putative Tail fiber protein), PP_3859 CDS (conserved protein of unknown function), PP_3860 CDS (Phage FluMu protein gp47), PP_3861 CDS (Phage FluMu protein gp46), PP_3862 CDS (putative Phage assembly protein), PP_3863 CDS (putative Phage tail protein), PP_3864 CDS (putative Phage FluMu DNA circulation protein), PP_3865 CDS (putative Phage tail protein), PP_5640 CDS (conserved protein of unknown function), PP_3866 CDS (conserved protein of unknown function), PP_3867 CDS (putative Phage tail tube protein), PP_3868 CDS (Group II intron‑encoding maturase), PP_3869 CDS (putative Phage sheath protein), PP_3870 CDS (Phage FluMu protein gp38), PP_3871 CDS (conserved protein of unknown function), PP_3873 CDS (conserved protein of unknown function), PP_3874 CDS (conserved protein of unknown function), PP_3875 CDS (conserved protein of unknown function), PP_3876 CDS (conserved protein of unknown function), PP_3877 CDS (conserved protein of unknown function), PP_3878 CDS (putative Phage minor capsid protein C), PP_3879 CDS (Phage portal protein_ HK97 family), PP_3880 CDS (conserved exported protein of unknown function), PP_3881 CDS (putative Phage terminase_ large subunit), PP_3882 CDS (putative Phage terminase_ small subunit), PP_3883 CDS (putative Phage holin), PP_3884 CDS (Phage holin_ lambda family), PP_5641 CDS (conserved protein of unknown function), PP_3886 CDS (conserved protein of unknown function), PP_3887 CDS (conserved protein of unknown function), PP_3888 CDS (conserved protein of unknown function), PP_3889 CDS (putative Phage integrase), PP_3890 CDS (conserved protein of unknown function), ybcO CDS (conserved protein of unknown function), PP_3892 CDS (conserved protein of unknown function), PP_3893 CDS (putative Phage DNA helicase), PP_3894 CDS (putative Phage replication protein O), PP_3895 CDS (putative Regulatory protein Cro), PP_3896 CDS (Transcriptional regulator_ Cro_CI family), PP_3897 CDS (conserved protein of unknown function), PP_3898 CDS (conserved protein of unknown function), PP_5643 CDS (conserved protein of unknown function), PP_5644 CDS (conserved membrane protein of unknown function), PP_3899 CDS (conserved protein of unknown function_ hicB family), PP_3900 CDS (hypothetical protein), PP_3901 CDS (conserved protein of unknown function), PP_3902 CDS (conserved protein of unknown function), PP_3903 CDS (conserved protein of unknown function), PP_3904 CDS (conserved protein of unknown function), PP_3905 CDS (Transcriptional regulator_ LuxR family), PP_3906 CDS (conserved protein of unknown function), PP_3907 CDS (conserved protein of unknown function), PP_3908 CDS (conserved protein of unknown function), PP_3909 CDS (conserved protein of unknown function), PP_3910 CDS (conserved protein of unknown function), PP_3911 CDS (conserved protein of unknown function), PP_3912 CDS (DNA‑cytosine methyltransferase), PP_3913 CDS (protein of unknown function), PP_5645 CDS (conserved protein of unknown function), PP_3914 CDS (conserved protein of unknown function), PP_5646 CDS (protein of unknown function), PP_3915 CDS (conserved protein of unknown function), PP_3916 CDS (conserved protein of unknown function), PP_5647 CDS (conserved protein of unknown function), PP_3917 CDS (conserved protein of unknown function), PP_3919 CDS (conserved protein of unknown function), [PP_3920 CDS (putative Phage integrase)]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451_phaM1	4372651	4427416	54766	793 [0]	[2] 875	PP_3849 CDS (putative calcium‑binding protein_ hemolysin‑type)–[PP_3920 CDS (putative Phage integrase)]	PP_3849 CDS (putative calcium‑binding protein_ hemolysin‑type),PP_3850 CDS (conserved protein of unknown function),PP_5637 CDS (conserved protein of unknown function),PP_3851 CDS (conserved protein of unknown function),PP_3852 CDS (conserved protein of unknown function with BNR domain),PP_5638 CDS (conserved protein of unknown function),PP_3854 CDS (Lysozyme),PP_5639 CDS (conserved exported protein of unknown function),PP_3855 CDS (conserved protein of unknown function),PP_3856 CDS (conserved protein of unknown function),PP_3857 CDS (conserved protein of unknown function),PP_3858 CDS (putative Tail fiber protein),PP_3859 CDS (conserved protein of unknown function),PP_3860 CDS (Phage FluMu protein gp47),PP_3861 CDS (Phage FluMu protein gp46),PP_3862 CDS (putative Phage assembly protein),PP_3863 CDS (putative Phage tail protein),PP_3864 CDS (putative Phage FluMu DNA circulation protein),PP_3865 CDS (putative Phage tail protein),PP_5640 CDS (conserved protein of unknown function),PP_3866 CDS (conserved protein of unknown function),PP_3867 CDS (putative Phage tail tube protein),PP_3868 CDS (Group II intron‑encoding maturase),PP_3869 CDS (putative Phage sheath protein),PP_3870 CDS (Phage FluMu protein gp38),PP_3871 CDS (conserved protein of unknown function),PP_3873 CDS (conserved protein of unknown function),PP_3874 CDS (conserved protein of unknown function),PP_3875 CDS (conserved protein of unknown function),PP_3876 CDS (conserved protein of unknown function),PP_3877 CDS (conserved protein of unknown function),PP_3878 CDS (putative Phage minor capsid protein C),PP_3879 CDS (Phage portal protein_ HK97 family),PP_3880 CDS (conserved exported protein of unknown function),PP_3881 CDS (putative Phage terminase_ large subunit),PP_3882 CDS (putative Phage terminase_ small subunit),PP_3883 CDS (putative Phage holin),PP_3884 CDS (Phage holin_ lambda family),PP_5641 CDS (conserved protein of unknown function),PP_3886 CDS (conserved protein of unknown function),PP_3887 CDS (conserved protein of unknown function),PP_3888 CDS (conserved protein of unknown function),PP_3889 CDS (putative Phage integrase),PP_3890 CDS (conserved protein of unknown function),ybcO CDS (conserved protein of unknown function),PP_3892 CDS (conserved protein of unknown function),PP_3893 CDS (putative Phage DNA helicase),PP_3894 CDS (putative Phage replication protein O),PP_3895 CDS (putative Regulatory protein Cro),PP_3896 CDS (Transcriptional regulator_ Cro_CI family),PP_3897 CDS (conserved protein of unknown function),PP_3898 CDS (conserved protein of unknown function),PP_5643 CDS (conserved protein of unknown function),PP_5644 CDS (conserved membrane protein of unknown function),PP_3899 CDS (conserved protein of unknown function_ hicB family),PP_3900 CDS (hypothetical protein),PP_3901 CDS (conserved protein of unknown function),PP_3902 CDS (conserved protein of unknown function),PP_3903 CDS (conserved protein of unknown function),PP_3904 CDS (conserved protein of unknown function),PP_3905 CDS (Transcriptional regulator_ LuxR family),PP_3906 CDS (conserved protein of unknown function),PP_3907 CDS (conserved protein of unknown function),PP_3908 CDS (conserved protein of unknown function),PP_3909 CDS (conserved protein of unknown function),PP_3910 CDS (conserved protein of unknown function),PP_3911 CDS (conserved protein of unknown function),PP_3912 CDS (DNA‑cytosine methyltransferase),PP_3913 CDS (protein of unknown function),PP_5645 CDS (conserved protein of unknown function),PP_3914 CDS (conserved protein of unknown function),PP_5646 CDS (protein of unknown function),PP_3915 CDS (conserved protein of unknown function),PP_3916 CDS (conserved protein of unknown function),PP_5647 CDS (conserved protein of unknown function),PP_3917 CDS (conserved protein of unknown function),PP_3919 CDS (conserved protein of unknown function),[PP_3920 CDS (putative Phage integrase)]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451_phaM1	= 4372650	0 (0.000)	753 (1.020)	117/264	0.3	99.9%	intergenic (‑1235/‑337)	PP_5636 CDS (protein of unknown function)/PP_3849 CDS (putative calcium‑binding protein_ hemolysin‑type)	–/–
*	?	AE015451_phaM1	4427417 =	1 (0.000)	753 (1.020)	117/264	0.3	99.9%	intergenic (+1/+101)	PP_3920 CDS (putative Phage integrase)/PP_t61 tRNA (tRNA‑Cys)	–/–

GATK/CNVnator alignment

N/A