Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F11 I4 R1 74 758.2 33768536 99.3% 33532156 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 4,586,057 +C intergenic (+140/+75) PP_4061 CDS (conserved protein of unknown function) → / ← PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,586,0561.C99.0% 396.8 / ‑3.1 103intergenic (+139/+76)PP_4061 CDS (conserved protein of unknown function)/PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (15/87);  total (15/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACT  >  AE015451_phaM1/4585923‑4586187
                                                                                                                                        |                                                                                                                                    
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCATGGA‑cc                                                                                                                                    <  2:3049154/137‑2 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                    <  1:16052116/137‑2 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                    <  2:14328740/137‑2 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                    <  1:5436404/137‑2 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                    <  2:6426239/137‑2 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑cc                                                                                                                                    <  2:3468347/137‑2 (MQ=39)
 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGTAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCATGGA‑ccg                                                                                                                                   <  2:12258082/137‑3 (MQ=38)
 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                   <  1:2118092/137‑3 (MQ=38)
 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                   <  1:378889/137‑3 (MQ=38)
 aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                   <  2:2153982/137‑3 (MQ=38)
 aCCACGGCACCGGCGTGAACAGATGATCTGCACTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                   <  1:2366770/137‑3 (MQ=38)
  ccACGGCACCGGCGTGAACAGCGGATTTGCACGGCTCCATTGAAGGGTGGAGGATGA‑CTGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccgtg                                                                                                                                 <  1:14997911/137‑5 (MQ=25)
   cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccgtg                                                                                                                                 <  1:10175231/137‑5 (MQ=38)
      ggCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCCTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCg                                                                                                                              <  2:11453755/137‑1 (MQ=38)
      ggCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGACGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCg                                                                                                                              <  2:11453721/137‑1 (MQ=38)
                  aaCAGTTGATCTGCGCGGCTCCAGTGAAAGTTTTAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:5353033/137‑1 (MQ=37)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGTATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:3479831/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:8792608/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:5468486/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:2901678/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:5726968/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:3479867/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:12384353/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:14720375/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:6897914/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:9148184/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:12030057/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:10816443/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:866389/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:11201592/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:8490875/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:7484436/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:9938772/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:1415154/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:9104124/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:16581300/137‑1 (MQ=38)
                  aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTCCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  1:13667774/137‑1 (MQ=38)
                  aaCAGCTGAGCTGCGCTGATCCATTGAAAGGTCGAGGATAACCGGGTGCTTGGCAAGGTGAAAACCTTGCATGGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:5206304/137‑1 (MQ=25)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTTGCAAGGTGAAGATCTTGCGTTGATCGGGCCGGTCGCGTCGCG‑GGTAAACCCGCTTCTACCAGGGA‑CTGTGCCGGCCTTGAATgcggcg                                                                                                               <  2:2149135/137‑1 (MQ=255)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:16547880/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:13016728/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:8257398/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:8030727/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:11275872/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:16057466/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:10951385/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:948703/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:16547920/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:10387600/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:8516368/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:11173003/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:13350844/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:14277771/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:10271670/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:7319464/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:1786245/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:1786277/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:2140111/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:5129658/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:7423039/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:5417129/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:6024659/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  1:6024697/137‑1 (MQ=38)
                     aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGAACGACCGGGTGCTTGGCAAGTTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCTGGTAAACCCGCTCCTACCATGGACC‑GTGCCGGCCTTGAATgcggcg                                                                                                               <  2:9365169/137‑1 (MQ=255)
                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  1:8283085/137‑1 (MQ=38)
                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  2:7482454/137‑1 (MQ=38)
                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  2:7080326/137‑1 (MQ=38)
                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  2:4186018/137‑1 (MQ=38)
                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  1:98543/137‑1 (MQ=38)
                       cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCt                                                                                                             <  1:10246407/137‑1 (MQ=38)
                        tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTg                                                                                                            <  1:8524261/137‑1 (MQ=38)
                        tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTg                                                                                                            <  2:3230527/137‑1 (MQ=38)
                        tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTg                                                                                                            <  1:5870022/137‑1 (MQ=38)
                         gATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGc                                                                                                           <  1:917887/137‑1 (MQ=38)
                         gATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGc                                                                                                           <  1:11062010/137‑1 (MQ=38)
                         gATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGc                                                                                                           <  1:16004065/137‑1 (MQ=38)
                         gATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGc                                                                                                           <  1:16004095/137‑1 (MQ=38)
                         gATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGc                                                                                                           <  1:8442098/137‑1 (MQ=38)
                             tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCt                                                                                                       <  1:819811/137‑1 (MQ=38)
                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  1:5165294/137‑1 (MQ=38)
                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  1:4994798/137‑1 (MQ=38)
                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  1:4927534/137‑1 (MQ=38)
                                 ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                                                                                                   <  1:1715444/137‑1 (MQ=38)
                                  tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  <  2:4824816/121‑1 (MQ=37)
                                  tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATgcg                                                                                                                  >  1:4824816/1‑121 (MQ=37)
                                  tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                                                                                                  <  1:11132982/137‑1 (MQ=38)
                                       cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                   >  1:15962527/1‑97 (MQ=37)
                                       cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGA‑ccg                                                                                                                                   <  2:15962527/99‑3 (MQ=37)
                                                                                                                              cTACCAGGGACC‑GTGCCGGCCTTGACTGCGGCGCTGCCCCTGGTAGGAGCGGGTTTCCCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACACGCAGATGCAGCCGCAATCCCCGCCACACTGATCTCACGCATGCg       >  2:5436462/1‑137 (MQ=255)
                                                                                                                              cTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCg       >  2:5436508/1‑137 (MQ=255)
                                                                                                                              cTACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCg       >  1:5926199/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAANCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:13650778/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:5306362/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  1:11354621/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:4377200/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:14547497/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACACATGCgg      >  1:3166063/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCAGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg      >  2:14547529/1‑137 (MQ=255)
                                                                                                                               tACCAGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCATGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTAATCTCACGCATGCgg      >  2:16011147/1‑137 (MQ=255)
                                                                                                                                   aGGGACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACt  >  1:4590263/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                    
GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACT  >  AE015451_phaM1/4585923‑4586187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGT  >  AE015451_phaM1/4585908‑4586203
                                                                                                                                                       |                                                                                                                                                       
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2245:22402:18310/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1238:39829:27645/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1251:24797:16596/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2202:11382:5660/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2230:38174:22377/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2230:46596:14259/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2233:27774:23354/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2202:11364:5660/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1124:22005:6316/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1153:31508:25371/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2258:16790:27934/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1167:40864:20824/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2263:5410:6268/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1205:34023:29311/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1246:50609:12113/151‑1 (MQ=60)
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2147:32895:12689/151‑1 (MQ=60)
 AAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑CC                                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2161:14978:4106/151‑1 (MQ=60)
 AAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑CC                                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2161:14969:4090/151‑1 (MQ=60)
 AAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑CC                                                                                                                                                        <  LH00941:114:22FVMWLT1:2:1203:2738:22393/151‑1 (MQ=60)
     CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGC                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1103:15847:17141/151‑1 (MQ=60)
     CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGC                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2161:21542:24635/151‑1 (MQ=60)
          GGTTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2146:5327:22457/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2244:19111:6364/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2146:5336:22473/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1113:8451:20920/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1257:5844:20279/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2229:17955:27197/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1214:24806:10784/151‑1 (MQ=60)
          GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGCGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2244:19092:6364/151‑1 (MQ=60)
               GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2257:41391:11873/151‑1 (MQ=60)
               GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                                           <  LH00941:114:22FVMWLT1:2:1221:49176:17605/151‑1 (MQ=60)
                ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                                          <  LH00941:114:22FVMWLT1:2:2145:13213:2681/151‑1 (MQ=60)
                ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                                          <  LH00941:114:22FVMWLT1:2:2259:43176:6252/151‑1 (MQ=60)
                ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1107:48353:11120/151‑1 (MQ=60)
                ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1136:11317:11088/151‑1 (MQ=60)
                ACCACGGCACCGGCGTGAACAGATGATCTGCACTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1140:27265:15203/151‑1 (MQ=60)
                 CCACGGCACCGGCGTGAACAGCGGATTTGCACGGCTCCATTGAAGGGTGGAGGATGA‑CTGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGC                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2240:16023:2681/151‑1 (MQ=60)
                  CACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGC                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2130:17872:7229/151‑1 (MQ=60)
                       CACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCTGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGC                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1216:3311:19255/151‑1 (MQ=60)
                           GGCGTGAACAGATGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2227:48482:22649/151‑1 (MQ=60)
                            GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                                              <  LH00941:114:22FVMWLT1:2:1249:49518:23418/151‑1 (MQ=60)
                            GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                                              <  LH00941:114:22FVMWLT1:2:2212:29197:6220/151‑1 (MQ=60)
                            GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                                              <  LH00941:114:22FVMWLT1:2:2216:32461:2745/151‑1 (MQ=60)
                                 AACAGTTGATCTGCGCGGCTCCAGTGAAAGTTTTAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1220:4633:7037/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGTATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1159:45986:3082/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1245:18076:21480/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2126:27811:8526/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2147:46790:11841/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2113:20553:2393/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1159:45995:3098/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2266:41438:2505/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2113:20535:2393/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2112:9681:9823/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2107:32858:4235/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2140:19490:28446/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1226:50396:21320/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1149:20932:12849/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1130:6482:24779/151‑1 (MQ=60)
                                 AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTCCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:2218:14109:6188/151‑1 (MQ=60)
                                 AACAGCTGATCTGCACTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                         <  LH00941:114:22FVMWLT1:2:1224:32119:10640/151‑1 (MQ=60)
                                  ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                                                                                                                        <  LH00941:114:22FVMWLT1:2:1251:1897:4058/151‑1 (MQ=60)
                                   CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTct                                                                                                                       >  LH00941:114:22FVMWLT1:2:1211:20267:14611/1‑149 (MQ=60)
                                    AGCTGGTCTGCGCTGCTCCATTGAAAGGTTGAGGATGGTCGGGTGCTTGGCAAGGGGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATGCTGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1254:32294:11809/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2265:32239:15492/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1268:29456:12433/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2212:30880:28958/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1221:50396:8574/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2133:26128:22521/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2146:5604:26877/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1116:32655:26332/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1130:16763:24843/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2131:29031:24603/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1268:14461:6092/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1264:40291:13938/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2265:32230:15508/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1136:36871:21464/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1254:32313:11809/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1130:16772:24827/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1231:38248:9407/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:2228:38257:6861/151‑1 (MQ=60)
                                    AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1231:38239:9391/151‑1 (MQ=60)
                                    AGCTGATCGGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCGCCTGCAATGGACCGTGCCGGCCTTGAATGCGGCGCGGCCCCTGGTAGG                                                                                                                      <  LH00941:114:22FVMWLT1:2:1254:32304:11793/151‑1 (MQ=60)
                                     ccccccccccccccccttttccaaaacaaaacccccccccctagatgctactatctgtctcgtgggctcggagatgtgtataagagacagGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGA                                                                                                                     <  LH00941:114:22FVMWLT1:2:2165:37277:19799/61‑1 (MQ=60)
                                      aaagccacccccctagatgctactatctgtctcgtgggctcggagatgtgtataagagacagTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                                                    <  LH00941:114:22FVMWLT1:2:1213:9311:24491/89‑1 (MQ=53)
                                      CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                                                    <  LH00941:114:22FVMWLT1:2:1268:5780:18278/151‑1 (MQ=60)
                                      CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                                                    <  LH00941:114:22FVMWLT1:2:1102:4670:19815/151‑1 (MQ=60)
                                      CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                                                    <  LH00941:114:22FVMWLT1:2:2131:22901:12673/151‑1 (MQ=60)
                                      CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                                                    <  LH00941:114:22FVMWLT1:2:2126:37832:17205/151‑1 (MQ=60)
                                      CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                                                    <  LH00941:114:22FVMWLT1:2:1214:9284:23770/151‑1 (MQ=60)
                                       TGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:2102:46513:18822/151‑1 (MQ=60)
                                       TGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGC                                                                                                                   <  LH00941:114:22FVMWLT1:2:1228:23327:22121/151‑1 (MQ=60)
                                        GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATctgtctcttatacacatctccgag                                                                                                                  >  LH00941:114:22FVMWLT1:2:2255:47207:27037/1‑127 (MQ=60)
                                        GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG                                                                                                                  <  LH00941:114:22FVMWLT1:2:1116:41373:11584/151‑1 (MQ=60)
                                        GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG                                                                                                                  <  LH00941:114:22FVMWLT1:2:2145:3746:3034/151‑1 (MQ=60)
                                        GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG                                                                                                                  <  LH00941:114:22FVMWLT1:2:2256:5909:17894/151‑1 (MQ=60)
                                        GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG                                                                                                                  <  LH00941:114:22FVMWLT1:2:2256:5918:17878/151‑1 (MQ=60)
                                        GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG                                                                                                                  <  LH00941:114:22FVMWLT1:2:2101:8479:7966/151‑1 (MQ=60)
                                            TGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTT                                                                                                              <  LH00941:114:22FVMWLT1:2:1247:21376:28478/151‑1 (MQ=60)
                                            TGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTT                                                                                                              <  LH00941:114:22FVMWLT1:2:1114:22744:21945/151‑1 (MQ=60)
                                                CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                                          <  LH00941:114:22FVMWLT1:2:1103:32137:15187/151‑1 (MQ=60)
                                                CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                                          <  LH00941:114:22FVMWLT1:2:1213:38599:6172/151‑1 (MQ=60)
                                                CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                                          <  LH00941:114:22FVMWLT1:2:1217:9163:4315/151‑1 (MQ=60)
                                                CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                                          <  LH00941:114:22FVMWLT1:2:1144:7176:2281/151‑1 (MQ=60)
                                                CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                                          <  LH00941:114:22FVMWLT1:2:1214:4495:9391/151‑1 (MQ=60)
                                                CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                                          <  LH00941:114:22FVMWLT1:2:1129:48806:19751/151‑1 (MQ=60)
                                                 TGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC                                                                                                         <  LH00941:114:22FVMWLT1:2:2146:38840:7934/151‑1 (MQ=60)
                                                 TGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC                                                                                                         <  LH00941:114:22FVMWLT1:2:2163:50165:21272/151‑1 (MQ=60)
                                                                                       ccccccagatgctactatctgtctcgtgggctcggagatgtgtataagagacagTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGA                                                              <  LH00941:114:22FVMWLT1:2:1239:7398:13394/97‑1 (MQ=60)
                                                                                                                             G‑GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCG                       >  LH00941:114:22FVMWLT1:2:1229:42621:20119/1‑151 (MQ=60)
                                                                                                                               GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAANCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG                      >  LH00941:114:22FVMWLT1:2:2217:31453:26877/1‑151 (MQ=60)
                                                                                                                               GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG                      >  LH00941:114:22FVMWLT1:2:1164:6482:3258/1‑151 (MQ=60)
                                                                                                                               GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG                      >  LH00941:114:22FVMWLT1:2:2149:33468:27645/1‑151 (MQ=60)
                                                                                                                               GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACACATGCGG                      >  LH00941:114:22FVMWLT1:2:1153:3635:24587/1‑151 (MQ=60)
                                                                                                                                   AACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACT                  >  LH00941:114:22FVMWLT1:2:1207:19721:18118/1‑151 (MQ=60)
                                                                                                                                        GCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGC             >  LH00941:114:22FVMWLT1:2:2257:37185:18454/1‑151 (MQ=60)
                                                                                                                                        GCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGC             >  LH00941:114:22FVMWLT1:2:2126:24316:22425/1‑151 (MQ=60)
                                                                                                                                         CTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCA            >  LH00941:114:22FVMWLT1:2:1128:45875:28126/1‑151 (MQ=60)
                                                                                                                                         CTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCA            >  LH00941:114:22FVMWLT1:2:2215:26017:9327/1‑151 (MQ=60)
                                                                                                                                         CTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCA            >  LH00941:114:22FVMWLT1:2:1128:45866:28110/1‑151 (MQ=60)
                                                                                                                                         CTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCA            >  LH00941:114:22FVMWLT1:2:1120:2183:6348/1‑151 (MQ=60)
                                                                                                                                          acagACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAC           <  LH00941:114:22FVMWLT1:2:1110:15321:15203/147‑1 (MQ=60)
                                                                                                                                           CCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACctgtctcttatacacatctccgagcccacgagacagatagtagcatctaggggggagtttttttt          >  LH00941:114:22FVMWLT1:2:2123:30316:29135/1‑86 (MQ=60)
                                                                                                                                           CCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACC          >  LH00941:114:22FVMWLT1:2:1128:22892:15027/1‑151 (MQ=60)
                                                                                                                                            CTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTG              >  LH00941:114:22FVMWLT1:2:2269:49786:27629/1‑151 (MQ=60)
                                                                                                                                            CTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTG              >  LH00941:114:22FVMWLT1:2:2106:21191:20952/1‑151 (MQ=60)
                                                                                                                                            CTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCT         >  LH00941:114:22FVMWLT1:2:2206:12482:29183/1‑151 (MQ=60)
                                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTT        >  LH00941:114:22FVMWLT1:2:2136:44831:2489/1‑151 (MQ=60)
                                                                                                                                             TACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTT        >  LH00941:114:22FVMWLT1:2:2266:10374:5131/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1209:37925:14034/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGCGGCCGGCCGCGC‑‑‑‑‑CCGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1222:19416:9070/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTgt       >  LH00941:114:22FVMWLT1:2:2225:11900:6172/1‑149 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2262:35955:22890/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2112:4014:19895/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2112:10328:12737/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1223:7185:16677/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1115:4162:27133/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2261:13250:17445/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2245:19055:6492/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1261:14683:17493/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1261:14664:17493/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1105:4005:21641/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1250:18861:1993/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1240:33200:22121/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2131:35391:20536/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1144:2747:17317/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2138:32923:10976/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1125:50119:4891/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1211:3468:11649/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2157:10670:26364/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1163:33894:8270/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1152:46652:15412/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2229:10014:8446/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2166:23687:21560/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2225:25046:14371/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2208:15191:9823/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACACCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:1149:14008:25612/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCATGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2258:29696:21048/1‑151 (MQ=60)
                                                                                                                                              ACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCATGGTAGGAGCGGGTTTACCCGCAAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG       >  LH00941:114:22FVMWLT1:2:2241:20627:28430/1‑151 (MQ=60)
                                                                                                                                                   GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGT  >  LH00941:114:22FVMWLT1:2:1136:49194:12673/1‑151 (MQ=60)
                                                                                                                                                       |                                                                                                                                                       
GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGC‑‑‑‑‑CAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGT  >  AE015451_phaM1/4585908‑4586203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑