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Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F73 I2 R1	76	833.2	37290668	99.4%	37066923	136.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451_phaM1	2,586,628	39195 bp→CCCCTTT		PP_2266 CDS (DNA‑directed RNA polymerase_ bacteriophage and mitochondrial type)–[PP_2297 CDS (putative integrative genetic element Ppu40_ integrase)]	42 genes PP_2266 CDS (DNA‑directed RNA polymerase_ bacteriophage and mitochondrial type), PP_5507 CDS (protein of unknown function), PP_2267 CDS (putative Phage single‑stranded DNA‑binding protein), PP_2268 CDS (Phage endodeoxyribonuclease I), PP_2269 CDS (putative N‑acetylmuramoyl‑L‑alanine amidase), PP_2270 CDS (putative DNA primase_helicase protein), PP_5508 CDS (conserved protein of unknown function), PP_5509 CDS (conserved exported protein of unknown function), PP_5510 CDS (protein of unknown function), PP_2272 CDS (conserved protein of unknown function), PP_5511 CDS (protein of unknown function), PP_2273 CDS (DNA polymerase I from phage origin), PP_2274 CDS (conserved protein of unknown function), PP_2275 CDS (conserved protein of unknown function), PP_2276 CDS (putative phage exonuclease), PP_2277 CDS (conserved protein of unknown function), PP_2278 CDS (conserved protein of unknown function), PP_5512 CDS (protein of unknown function), PP_2279 CDS (Head‑to‑tail joining protein), PP_2280 CDS (conserved protein of unknown function), PP_2281 CDS (putative Capsid assembly protein), PP_5513 CDS (conserved protein of unknown function of phage origin), PP_2282 CDS (Minor capsid protein 10), PP_2283 CDS (Tail tubular protein A), PP_2284 CDS (Tail tubular protein B), PP_5514 CDS (conserved protein of unknown function), PP_2285 CDS (conserved exported protein of unknown function), PP_2286 CDS (conserved protein of unknown function), PP_2287 CDS (Phage internal core protein), PP_2288 CDS (Phage tail fiber protein), PP_2289 CDS (conserved exported protein of unknown function), PP_2290 CDS (conserved protein of unknown function), PP_2291 CDS (conserved protein of unknown function), PP_2292 CDS (conserved protein of unknown function), PP_2293 CDS (DNA maturase B), PP_2294 CDS (conserved exported protein of unknown function), PP_5515 CDS (protein of unknown function), PP_5516 CDS (protein of unknown function), PP_5517 CDS (conserved exported protein of unknown function), PP_2295 CDS (putative antirestriction protein), PP_2296 CDS (conserved protein of unknown function), [PP_2297 CDS (putative integrative genetic element Ppu40_ integrase)]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451_phaM1	2586628	2625822	39195	868 [3]	[0] 1021	PP_2266 CDS (DNA‑directed RNA polymerase_ bacteriophage and mitochondrial type)–[PP_2297 CDS (putative integrative genetic element Ppu40_ integrase)]	PP_2266 CDS (DNA‑directed RNA polymerase_ bacteriophage and mitochondrial type),PP_5507 CDS (protein of unknown function),PP_2267 CDS (putative Phage single‑stranded DNA‑binding protein),PP_2268 CDS (Phage endodeoxyribonuclease I),PP_2269 CDS (putative N‑acetylmuramoyl‑L‑alanine amidase),PP_2270 CDS (putative DNA primase_helicase protein),PP_5508 CDS (conserved protein of unknown function),PP_5509 CDS (conserved exported protein of unknown function),PP_5510 CDS (protein of unknown function),PP_2272 CDS (conserved protein of unknown function),PP_5511 CDS (protein of unknown function),PP_2273 CDS (DNA polymerase I from phage origin),PP_2274 CDS (conserved protein of unknown function),PP_2275 CDS (conserved protein of unknown function),PP_2276 CDS (putative phage exonuclease),PP_2277 CDS (conserved protein of unknown function),PP_2278 CDS (conserved protein of unknown function),PP_5512 CDS (protein of unknown function),PP_2279 CDS (Head‑to‑tail joining protein),PP_2280 CDS (conserved protein of unknown function),PP_2281 CDS (putative Capsid assembly protein),PP_5513 CDS (conserved protein of unknown function of phage origin),PP_2282 CDS (Minor capsid protein 10),PP_2283 CDS (Tail tubular protein A),PP_2284 CDS (Tail tubular protein B),PP_5514 CDS (conserved protein of unknown function),PP_2285 CDS (conserved exported protein of unknown function),PP_2286 CDS (conserved protein of unknown function),PP_2287 CDS (Phage internal core protein),PP_2288 CDS (Phage tail fiber protein),PP_2289 CDS (conserved exported protein of unknown function),PP_2290 CDS (conserved protein of unknown function),PP_2291 CDS (conserved protein of unknown function),PP_2292 CDS (conserved protein of unknown function),PP_2293 CDS (DNA maturase B),PP_2294 CDS (conserved exported protein of unknown function),PP_5515 CDS (protein of unknown function),PP_5516 CDS (protein of unknown function),PP_5517 CDS (conserved exported protein of unknown function),PP_2295 CDS (putative antirestriction protein),PP_2296 CDS (conserved protein of unknown function),[PP_2297 CDS (putative integrative genetic element Ppu40_ integrase)]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451_phaM1	= 2586627	3 (0.000)	863 (1.090) +CCCCTTT	126/256	0.1	99.8%	intergenic (+533/‑9)	PP_t39 tRNA (tRNA‑Leu)/PP_2266 CDS (DNA‑directed RNA polymerase_ bacteriophage and mitochondrial type)	–/–
*	?	AE015451_phaM1	2625823 =	0 (0.000)	863 (1.090) +CCCCTTT	126/256	0.1	99.8%	intergenic (+1/‑299)	PP_2297 CDS (putative integrative genetic element Ppu40_ integrase)/PP_t40 tRNA (tRNA‑His)	–/–

GATK/CNVnator alignment

N/A